[Home ] [Archive]    
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
:: Volume 10, Issue 3 (Int J Mol Cell Med (In Press) 2021) ::
Int J Mol Cell Med 2021, 10(3): 0-0 Back to browse issues page
Evaluation of FOXCUT, CCAT2, and HULC LncRNA Expression Levels and Apoptosis Induction by Sodium Butyrate in PC-3 and LNCAP Prostate Cancer Cell Lines
Sanaz Kavousi1 , Seyed Ataollah Sadat Shandiz 2, Nastaran Asghari Moghaddam1
1- Department of Biology, Central Tehran Branch, Islamic Azad University, Tehran, Iran.
2- Department of Biology, Central Tehran Branch, Islamic Azad University, Tehran, Iran. , Ata.sadatshandiz@iauctb.ac.ir
Abstract:   (596 Views)
Sodium butyrate (NaBu) is a short-chain fatty acid acting as a histone deacetylase inhibitor, and has been shown to be a potential regulator of cancer cell death. This study aimed to evaluate the effect of NaBu on cell cycle control, apoptosis, and expression of some lncRNAs in two human prostate cancer cells (PC-3 and LNCAP). Cell viability was assessed and the appropriate dose was determined using the MTT assay. Real-time PCR technique was also used to evaluate the expression levels of HULC, FOXCUT, and CCAT2 lncRNAs. Apoptosis was diagnosed using annexin V staining, and cell cycle distribution was then assessed using flow cytometry with propidium iodide DNA staining. NaBu induced apoptosis in both prostate cancer cell lines in a dose-dependent manner. The expressions of CCAT2 and HULC lncRNAs genes have significantly decreased in the presence of NaBu (P <0.05) in both PC3 and LNCAP cell lines, in comparison with the control. However, no significant difference was observed in the expression of FOXCUT lncRNAs. Moreover, the results of flow cytometry showed an increase in cell cycle arrest of LNCAP cell line at the sub-G1 stage as compared to the control cells, but no significant difference was observed between the control cells and NaBu-exposed PC-3 cells. In addition, the percentages of early and late apoptotic cells following treatment with NaBu were 80% and 49.63% in LNCAP and PC-3 cells, respectively. Our results suggest that NaBu has a positive effect on the induction of apoptosis and inhibition of cell cycle in PC-3 and LNCAP prostate cancer cells.
Keywords: Prostatic neoplasms, RNA, long noncoding RNA, apoptosis
Full-Text [PDF 725 kb]   (137 Downloads)    
Type of Study: Original Article | Subject: Cell Biology
Received: 2021/04/20 | Accepted: 2021/09/25 | Published: 2021/08/19
Send email to the article author

Add your comments about this article
Your username or Email:

CAPTCHA


XML     Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

kavousi S, Sadat Shandiz S A, Asghari Moghaddam N. Evaluation of FOXCUT, CCAT2, and HULC LncRNA Expression Levels and Apoptosis Induction by Sodium Butyrate in PC-3 and LNCAP Prostate Cancer Cell Lines. Int J Mol Cell Med. 2021; 10 (3)
URL: http://ijmcmed.org/article-1-1623-en.html


Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Volume 10, Issue 3 (Int J Mol Cell Med (In Press) 2021) Back to browse issues page
International Journal of Molecular and Cellular Medicine (IJMCM) International Journal of Molecular and Cellular Medicine (IJMCM)
Persian site map - English site map - Created in 0.04 seconds with 29 queries by YEKTAWEB 4355