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:: Volume 8, Issue 4 (Int J Mol Cell Med 2019) ::
Int J Mol Cell Med 2019, 8(4): 240-246 Back to browse issues page
Diagnostic Value of Plasma Long Non-coding RNA HOTTIP as a Non-invasive Biomarker for Colorectal Cancer ( A Case- Control Study)
Soheila Ali akbar- Esfahani1, Morteza Karimipoor2, Fatemeh Bahreini1, Ali Reza Soltanian3, Najme Aletaha4, Ali Mahdavinezhad 5
1- Department of Molecular Medicine & Genetics, Hamadan University of Medical Sciences, Hamadan, Iran.
2- Molecular Medicine Department, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran.
3- Modelling of Non communicable Diseases Research Center, School of Public Health, Hamadan University of Medical Sciences, Hamadan, Iran.
4- Department of Gastroenterology, Imam Khomeini Hospital, Tehran University of Medical Science, Tehran, Iran.
5- Research center for Molecular Medicine, Department of Molecular Medicine and Genetics, Hamadan University of Medical Sciences, Hamadan, Iran. , alimahdavin@gmail.com
Abstract:   (1430 Views)
Long non-coding RNAs (lncRNAs) associated with various cancers, including colorectal cancer (CRC), could be collected from body fluids easily. Our aims were to determine the expression level of HOTTIP lncRNA in plasma samples of healthy individuals and CRC patients as well as their relationship with clinico-pathological characteristics of patients. First, total RNA was extracted from the plasma samples of 100 subjects including 50 patients and 50 age and sex matched healthy persons. Then, gene expression was measured using real-time PCR technique. The sensitivity and specificity of HOTTIP dysregulation in CRC and healthy individual’s plasma was measured by receiver operating characteristic (ROC) analysis. As compared with healthy controls, HOTTIP lncRNA was over expressed in a statistically significant manner in plasma samples of patients (P = 0.001). Significant relationship between HOTTIP expression and positive family history of CRC was observed, too (P = 0.04). The ROC curve analysis showed an AUC value of 0.775, a specificity of 82%, a sensitivity of 76%, with a cut off value equal to 2.40 (P = 0.001). HOTTIP transcript can be proposed as a new biomarker for early diagnosis due to the increased expression in plasma samples of patients with CRC and the relatively high sensitivity and specificity.
Keywords: Biomarker, colorectal neoplasms, long non-coding RNAs, lncRNA HOTTIP
Full-Text [PDF 96 kb]   (189 Downloads)    
Type of Study: Original Article | Subject: Biomarkers (diagnosis & treatment)
Received: 2019/08/7 | Accepted: 2020/01/12 | Published: 2020/05/29
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Ali akbar- Esfahani S, Karimipoor M, Bahreini F, Soltanian A R, Aletaha N, Mahdavinezhad A. Diagnostic Value of Plasma Long Non-coding RNA HOTTIP as a Non-invasive Biomarker for Colorectal Cancer ( A Case- Control Study). Int J Mol Cell Med. 2019; 8 (4) :240-246
URL: http://ijmcmed.org/article-1-1141-en.html


Volume 8, Issue 4 (Int J Mol Cell Med 2019) Back to browse issues page
International Journal of Molecular and Cellular Medicine (IJMCM) International Journal of Molecular and Cellular Medicine (IJMCM)
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