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:: Volume 8, Issue 4 (Int J Mol Cell Med 2019) ::
Int J Mol Cell Med 2019, 8(4): 271-282 Back to browse issues page
A Cardiac Troponin T Biosensor Based on Aptamer Self-assembling on Gold
Masoud Negahdary1, Mostafa Behjati-Ardakani2, Hossein Heli3, Naghmeh Sattarahmady 4
1- Nanomedicine and Nanobiology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran; Yazd Cardiovascular Research Center, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
2- Yazd Cardiovascular Research Center, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
3- Nanomedicine and Nanobiology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
4- Nanomedicine and Nanobiology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran; Department of Medical Physics, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran. , nsattar@sums.ac.ir
Abstract:   (618 Views)
In this study, a sensitive and accurate aptasensor was designed for early detection of myocardial infarction through the determination of troponin T (TnT). The successful immobilization of a specific aptamer sequence on the surface of gold that had a high affinity toward TnT was accomplished. TnT was electrochemically quantified. The results indicated that the aptasensor detected TnT in a range of 0.05-5 ng mL and with a detection limit of 0.01 ng/mL. The performance of the aptasensor was investigated by analyzing 99 human serum samples. Both diagnostic specificity and sensitivity of the aptasensor were found to be 95%. The use of the designed aptamer-based biosensor could be an essential achievement in health policy, preventing deaths caused by myocardial infarction, and reducing patients with heart failure. The extensive use of this aptamer-based biosensor can also reduce costs, enhance speed, and improve accuracy in the diagnosis of TnT as an important myocardial infarction biomarker.
Keywords: Myocardial infarction, aptasensing, biomarker, bioelectrochemical detection
Full-Text [PDF 300 kb]   (199 Downloads)    
Type of Study: Original Article | Subject: Biomarkers (diagnosis & treatment)
Received: 2019/12/28 | Accepted: 2020/04/13 | Published: 2020/05/29
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Negahdary M, Behjati-Ardakani M, Heli H, Sattarahmady N. A Cardiac Troponin T Biosensor Based on Aptamer Self-assembling on Gold. Int J Mol Cell Med. 2019; 8 (4) :271-282
URL: http://ijmcmed.org/article-1-1222-en.html


Volume 8, Issue 4 (Int J Mol Cell Med 2019) Back to browse issues page
International Journal of Molecular and Cellular Medicine (IJMCM) International Journal of Molecular and Cellular Medicine (IJMCM)
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