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:: Volume 8, Issue 4 (Int J Mol Cell Med 2019) ::
Int J Mol Cell Med 2019, 8(4): 283-293 Back to browse issues page
Proliferation, Characterization and Differentiation Potency of Adipose Tissue-Derived Mesenchymal Stem Cells (AT-MSCs) Cultured in Fresh Frozen and non-Fresh Frozen Plasma
Wahyu Widowati 1, Rachmawati Noverina2, Wireni Ayuningtyas3, Dedy Kurniawan4, Hanna Sari Widya Kusuma5, Seila Arumwardana5, Dwi Surya Artie5, Ika Adhani Sholihah5, Rr. Anisa Siwianti Handayani5, Dian Ratih Laksmitawati6, Ratih Rinendyaputri7, Rilianawati Rilianawati8, Ahmad Faried9
1- Medical Research Center, Faculty of Medicine, Maranatha Christian University, Bandung, West Java, Indonesia , wahyu_w60@yahoo.com
2- 1. Animal and Stem Cells Laboratory, PT Bio Farma (Persero), Bandung 40161, West Java, Indonesia; 2. Doctoral Program, Faculty of Medicine, Universitas Padjadjaran, Bandung, West Java, Indonesia
3- 1. Animal and Stem Cells Laboratory, PT Bio Farma (Persero), Bandung 40161, West Java, Indonesia; 2. Magister Program, Faculty of Medicine, Universitas Padjadjaran, Bandung, West Java, Indonesia
4- Animal and Stem Cells Laboratory, PT Bio Farma (Persero), Bandung 40161, West Java, Indonesia
5- Biomolecular and Biomedical Research Center, Aretha Medika Utama, Bandung, West Java, Indonesia
6- Faculty of Pharmacy, Pancasila University, Jakarta, Indonesia
7- National Institute of Health Research and Development, Ministry of Health, Jakarta, Indonesia
8- Agency for the Assesment and Application of Technology, Ministry of Research and Technology, Serpong, Indonesia
9- Department of Neurosurgery and Stem Cell Working Group, Faculty of Medicine, Universitas Padjadjaran-Dr. Hasan Sadikin Hospital, Bandung, West Java, Indonesia
Abstract:   (885 Views)
Mesenchymal stem cells (MSCs) have unique properties, including high proliferation rates, self-renewal, and multilineage differentiation ability. Their characteristics are affected by increasing age and microenvironment. This research aimed to determine the proliferation, characteristics and differentiation capacity of adipose tissue-derived (AT)-MSCs at many passages with different media. The cell proliferation capacity was assayed using trypan blue. MSCs characterization (CD90, CD44, CD105, CD73, CD11b, CD19, CD34, CD45, and HLA-DR) was performed by flow cytometry, and cell differentiation was determined by specific stainings. Population doubling time (PDT) of AT-MSCs treated with fresh frozen plasma (FFP) and non-FFP increased in the late passage (P) (P15 FFP was 22.67 ± 7.01 days and non-FFP was 19.65 ± 2.27 days). Cumulative cell number was significantly different between FFP and non-FFP at P5, 10, 15. AT-MSCs at P4-15 were positive for CD90, CD44, CD105, and CD73, and negative for CD11b, CD19, CD34, CD45, and HLA-DR surface markers. AT-MSCs at P5, 10, 15 had potential toward adipogenic, chondrogenic, and osteogenic differentiation. Therefore, PDT was affected by increased age but no difference was observed in morphology, surface markers and differentiation capacity among passages. Cumulative cell number in FFP was higher in comparison with non-FFP in P5, 10, 15. Our data suggest that FFP may replace FBS for culturing MSCs.
Keywords: AT-MSCs, multilineage differentiation, PDT, proliferation, surface marker
Full-Text [PDF 525 kb]   (206 Downloads)    
Type of Study: Original Article | Subject: Molecular & Cellular Immunology
Received: 2019/12/13 | Accepted: 2020/04/2 | Published: 2020/05/29
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Widowati W, Noverina R, Ayuningtyas W, Kurniawan D, Sari Widya Kusuma H, Arumwardana S, et al . Proliferation, Characterization and Differentiation Potency of Adipose Tissue-Derived Mesenchymal Stem Cells (AT-MSCs) Cultured in Fresh Frozen and non-Fresh Frozen Plasma. Int J Mol Cell Med. 2019; 8 (4) :283-293
URL: http://ijmcmed.org/article-1-1215-en.html


Volume 8, Issue 4 (Int J Mol Cell Med 2019) Back to browse issues page
International Journal of Molecular and Cellular Medicine (IJMCM) International Journal of Molecular and Cellular Medicine (IJMCM)
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