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Has_circ_0008285/miR-211-5p/SIRT-1 Axis Suppress Ovarian Cancer Cells Progression
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Khadijeh Elmizadeh1   , Ali Homaei2 , Ensiyeh Bahadoran3 , Farzaneh Abbasi3 , Sahar Moghbelinejad 4 |
1- Department of Obstetrics and Gynecology, School of Medicine, Qazvin University of Medical Science, Qazvin, Iran.
2- Surgery Department, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, USA.
3- School of Medicine, Qazvin University of Medical Sciences, Qazvin, Iran.
4- Cellular and Molecular Research Centre, Research Institute for Prevention of Non-Commun-icable Diseases, Qazvin University of Medical Sciences, Qazvin, Iran. , smoghbelinejad@qums.ac.ir |
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Abstract: (521 Views) |
| The significant functional role of circular RNAs (circRNAs) in the progression of malignant tumors, including ovarian cancer, has been shown in various studies. In this study, we aimed to investigate the abnormal expression of hsa_circ_0008285 and its role in ovarian cancer pathogenesis. Quantitative real time polymerase chain reaction (qRT-PCR) and Western blot methods were used to detect the expression of hsa_circ_0008285 and some target genes in ovarian cancer tissues and related cell lines. To determine the functional roles of hsa_circ_0008285 in ovarian cancer, cell proliferation, apoptosis, and cell invasion assays were performed. Bioinformatics (Target scan, circ intractome) and luciferase reporter analyses were used to predict target genes. Results: In the present study, we first found that hsa_circ_0008285 was up regulated in ovarian cancer tissues and related cell lines. Bioinformatics, experimental data, and luciferase reporter analysis data showed miR-211-5p is a direct target of hsa_circ_0008285, while SIRT-1 is a direct target of miR-211-5p. Overexpression of hsa_circ_0008285 in cancer cells increased the expression of SIRT-1 and progression of cancer cells. Based on these results, inhibition of hsa_circ_0008285 expression could cause upregulation of miR-211-5p and down regulation of SIRT-1 and inhibited the proliferation and invasion of ovarian cancer cells. Conclusion: The results of the present study revealed that hsa_circ_0008285 suppressed ovarian cancer progression by regulating miR-211-5p expression to inhibit SIRT-1 expression. |
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| Keywords: Hsa_circ_0008285, ovarian cancer, miR-211-5p, SIRT-1 |
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Full-Text [PDF 1151 kb]
(153 Downloads)
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Type of Study: Original Article |
Subject:
Cancer
Received: 2024/01/10 | Accepted: 2024/04/6 | Published: 2024/05/20
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