[صفحه اصلی ]   [Archive]  
:: صفحه اصلي :: درباره نشريه :: آخرين شماره :: تمام شماره‌ها :: جستجو :: ثبت نام :: ارسال مقاله :: تماس با ما ::
:: دوره 1، شماره 1 - ( 3-1391 ) ::
برگشت به فهرست نسخه ها
Induction of secondary axis in hydra revisited: New insights into pattern formation
:   (15144 مشاهده)

In 1909, several years before the famous `Organizer’ experiments of Spemann and Mangold, Ethel Browne demonstrated induction of a secondary axis in hydra by grafting a hypostome. Based on this and subsequent work, in the late sixties, Lewis Wolpert proposed the theory of morphogen gradients and positional information. We have studied secondary axis induction by hypostome and foot tissue using three species of hydra as well as transgenic, GFP-expressing lines of hydra. We have found that pieces of hypostome and complete foot of a donor hydra can induce a secondary axis all along (in upper, middle or lower parts of) the body column of a host hydra, both within and across species with comparable rates. Thus, contrary to the available literature, our results show that the host hypostome does not completely inhibit the induction of a secondary axis. The length of the induced axis though is determined by the position of the graft. By using GFP-expressing lines of hydra we have demonstrated that host ectodermal and endodermal cells actively contribute to the secondary axis. On comparison, the hypostome was found to be a stronger and dominant Organizer than the foot. Foot grafting experiments show a transient increase in the host length as well as the distance between the two Organizers. The length becomes normal once the grafted foot reaches the budding zone. Our work brings out several new aspects of the role of positional cues in pattern formation in hydra that can be now be explored at cellular and molecular levels.

متن کامل [PDF 300 kb]   (2539 دریافت)    
نوع مطالعه: Original Article | موضوع مقاله: Cell Biology
دریافت: ۱۳۹۰/۱۰/۶ | پذیرش: ۱۳۹۲/۶/۲۳ | انتشار: ۱۳۹۲/۶/۲۳
ارسال نظر درباره این مقاله
نام کاربری یا پست الکترونیک شما:

CAPTCHA


XML   English Abstract   Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Kadu V, Ghaskadbi S S, Ghaskadbi S. Induction of secondary axis in hydra revisited: New insights into pattern formation. Int J Mol Cell Med. 2012; 1 (1) :11-20
URL: http://ijmcmed.org/article-1-25-fa.html

Induction of secondary axis in hydra revisited: New insights into pattern formation. مجله بین المللی سلولی و مولکولی. 1391; 1 (1) :11-20

URL: http://ijmcmed.org/article-1-25-fa.html



دوره 1، شماره 1 - ( 3-1391 ) برگشت به فهرست نسخه ها
International Journal of Molecular and Cellular Medicine (IJMCM) International Journal of Molecular and Cellular Medicine (IJMCM)
Persian site map - English site map - Created in 0.05 seconds with 32 queries by YEKTAWEB 4030