دوره 3، شماره 3 - ( 3-1393 )                   | برگشت به فهرست نسخه ها

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Behrouzi A, Bouzari S, Siadat S D, Irani S. In Silico Studies of Outer Membrane of Neisseria Meningitidis PorA: Its Expression and Immunogenic Properties. Int J Mol Cell Med 2014; 3 (3) :166-175
URL: http://ijmcmed.org/article-1-163-fa.html
In Silico Studies of Outer Membrane of Neisseria Meningitidis PorA: Its Expression and Immunogenic Properties. مجله بین المللی سلولی و مولکولی. 1393; 3 (3) :166-175

URL: http://ijmcmed.org/article-1-163-fa.html

In Silico Studies of Outer Membrane of Neisseria Meningitidis PorA: Its Expression and Immunogenic Properties
:   (10257 مشاهده)
Neisseria meningitidis is a major causative agent of bacterial septicemia and meningitis in humans. Currently, there are no vaccines to prevent disease caused by strains of N.meningitidis serogroup B. The Class 1 Outer Membrane Protein (OMP) has been named porA which is a cation selective transmembrane protein of 45 KDa that forms trimeric pore in the meningococcal outer membrane. PorA from serogroup B N. meningitidis was cloned into prokaryotic expression vector pBAD-gIIIA. Recombinant protein was expressed with arabinose and affinity purified by Ni-NTA agarose, SDS-PAGE and western blotting were performed for protein determination and verification. BALB/c mice were immunized subcutaneously with purified rPorA together with alum adjuvant. Serum antibody responses to serogroups B N.meningitidis were determined by ELISA. Serum IgG response significantly increased in the group immunized with rPorA together with alum adjuvant in comparison with control groups. These results suggest that rPorA can be a potential vaccine candidate for serogroup B N.meningitidis.
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نوع مطالعه: Original Article | موضوع مقاله: Molecular & Cellular Immunology
دریافت: 1393/2/6 | پذیرش: 1393/4/8 | انتشار: 1393/5/1
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