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Determination of ctxAB expression in Vibrio cholerae Classical and El Tor strains using Real-Time PCR
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| Seyed Mahmoud Amin Marashi1, Ramazan Rajabnia2, Abbas Ali Imani Fooladi3, Zohreh Hojati4, Sharareh Moghim5, Bahram Nasr Esfahani 6 |
1- Cellular and Molecular Biology Research Center (CMBRC), Babol University of Medical Sciences, Babol, Iran.
2- Infectious Diseases & Tropical Medicine Research Center, Department of Microbiology and Immunology, Babol University of Medical Sciences, Babol, Iran.
3- Applied Microbiology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran.
4- Genetics Division, Biology Department, Faculty of Sciences, University of Isfahan, Isfahan, Iran.
5- Department of Microbiology, Isfahan University of Medical Sciences, Isfahan, Iran.
6- Department of Microbiology, Isfahan University of Medical Sciences, Isfahan, Iran. , Nasr@hlth.mui.ac.ir |
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Abstract: (10828 Views) |
| Cholera is an infection of the small intestines caused by the bacterium V. cholerae. It is a major cause of health threat and also a major cause of death worldwide and especially in developing countries. The major virulence factor produced by V. cholerae during infection is the cholera toxin. Total mRNA extraction and reverse transcription was performed for making ctxAB cDNA. Relative Real-Time PCR analysis showed unequal enterotoxin production in V. cholerae strains. The results showed that, classical strain produces cholera toxin more than El Tor strain. |
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| Keywords: Vibrio cholerae, RT-qPCR, ctxAB expression |
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Full-Text [PDF 128 kb]
(3378 Downloads)
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Type of Study: Original Article |
Subject:
Infectious disease (Molecular and Cellular aspects)
Received: 2013/03/5 | Accepted: 2013/06/22 | Published: 2013/06/22
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