:: Volume 4, Issue 4 (Int J Mol Cell Med 2015) ::
Int J Mol Cell Med 2015, 4(4): 209-217 Back to browse issues page
Expression Pattern of Neuronal Markers in PB-MSCs Treated by Growth Factors Noggin, bFGF and EGF
Zahra Fazeli1, Masoumeh Rajabibazl1, Siamak Salami2, Nader Vazifeh Shiran2, Sayyed Mohammad Hossein Ghaderian3, Mir Davood Omrani 4
1- Department of Medical Genetics, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
2- Department of Clinical Biochemistry, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
3- Department of Hematology, School of Allied Medical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
4- Department of Medical Genetics, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran. , davood_omrani@yahoo.co.uk
Abstract:   (6871 Views)

Mesenchymal stem cells (MSCs) have the ability to differentiate into neuronal like cells under appropriate culture condition. In this study, we investigated whether MSCs derived from human peripheral blood (PB-MSCs) can differentiate into neuronal like cells by synergic effect of the growth factors EGF, bFGF and Noggin. For this purpose, the expression of five neuronal markers (Nestin, &beta III tubulin, NFM, MAP2 and NSE) were evaluated in treated PB-MSCs by SYBR Green Real time PCR. The expression analysis showed a higher expression of &beta- tubulin and NFM in treated BP-MSCs compared with untreated PB-MSCs as a control group. The expression of Nestin was also diminished in PB-MSCs treated with Noggin. This study suggested that the treatment of PB- MSCs with Noggin alongside with bFGF and EGF might differentiate these cells into neuronal lineage cells. The obtained results could be further developed for useful applications in regenerative medicine.

Keywords: Mesenchymal Stem Cells, Differentiation, Neuronal markers, Noggin
Full-Text [PDF 158 kb]   (1931 Downloads)    
Type of Study: Original Article | Subject: Stem Cell
Received: 2015/09/15 | Accepted: 2015/10/13 | Published: 2015/12/26


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Volume 4, Issue 4 (Int J Mol Cell Med 2015) Back to browse issues page