:: Volume 8, Issue 4 (Int J Mol Cell Med [In press] 2019) ::
Int J Mol Cell Med 2019, 8(4): 0-0 Back to browse issues page
AMELX Gene Association with Dental Caries in Iranian Adults
Fatemeh Koohpeima1 , Maryam Derakhshan1 , Mohammad Javad Mokhtari 2
1- Department of Operative Dentistry, Biomaterial Research Center, School of Dentistry, Shiraz University of Medical Sciences, Shiraz, Iran.
2- Young Researchers and Elite Club, Department of Biology, Zarghan Branch, Islamic Azad University, Zarghan, Iran. , mj.mokhtari@gmail.com
Abstract:   (574 Views)
Dental decay is a disease that is greatly affected by environmental components, but recently there have been an increasing number of documents supporting a genetic factor in the development of caries. The purpose of this study was to examine the association between dental caries and single-nucleotide polymorphisms in the AMELX gene. This research was carried out on 360 individuals of both sexes, who were referred to the dental school at the Shiraz University of Medical Sciences. In this research, individuals aged from 20–65 years were divided into two groups: controls (decayed, missed, or filled teeth (DMFT) ≤ 5; n = 180) and cases (DMFT ≥ 14; n = 180). The tetra-primer ARMS-PCR technique was performed for genotyping the DNA extracted from blood cells. Analysis of the AMELX rs946252 polymorphism showed that the T allele of rs946252 was a significant protective factor against dental caries in Iranian adults (T vs. C: OR = 0.70, 95% CI: 0.49–0.98, P = 0.04). We demonstrated the significant differences in the genotype frequencies under two genetic models: overdominant (TC vs. TT + CC: OR 0.35, 95% CI 0.19–0.64, P = 0.0006) and recessive (CC vs. TC + TT: OR 2.57, 95% CI 1.39–4.76, P = 0.002). Our results show that the SNPs of the AMELX gene may be related with susceptibility to dental caries in Iranian adults.
Keywords: Dental caries, DMFT, AMELX, rs946252, Tetra-primer ARMS-PCR
     
Type of Study: Original Article | Subject: Genetics & Disease
Received: 2019/06/29 | Accepted: 2020/01/22


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Volume 8, Issue 4 (Int J Mol Cell Med [In press] 2019) Back to browse issues page