دوره 5، شماره 2 - ( 1-1395 )                   | برگشت به فهرست نسخه ها


XML English Abstract Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Zaker F, Amirizadeh N, Nasiri N, Razavi S M, Teimoori-Toolabi L, Yaghmaie M et al . Gene Expression and Methylation Pattern in HRK Apoptotic Gene in Myelodysplastic Syndrome. Int J Mol Cell Med 2016; 5 (2) :90-99
URL: http://ijmcmed.org/article-1-497-fa.html
Gene Expression and Methylation Pattern in HRK Apoptotic Gene in Myelodysplastic Syndrome. مجله بین المللی سلولی و مولکولی. 1395; 5 (2) :90-99

URL: http://ijmcmed.org/article-1-497-fa.html


:   (9455 مشاهده)

Myelodysplastic syndromes (MDSs) are a clonal bone marrow (BM) disease characterized by ineffective hematopoiesis, dysplastic maturation and progression to acute myeloid leukemia (AML). Methylation silencing of HRK has been found in several human malignancies. In this study, we explored the association of HRK methylation status with its expression, clinical parameters and MDS subtypes in MDS patients. To study the methylation status of HRK gene, we applied Methylation Sensitive-High Resolution Melting Curve Analysis (MS-HRM) in MDS patients, as well as healthy controls and EpiTect®PCR Control DNA. Real time RT-PCR was used for gene expression analysis. Methylation frequency in promoter region of HRK in patient samples was 20.37%. Methylation of HRK was significantly related to transcriptional downregulation (P=0.023). The difference in frequency of hypermethylated HRK gene was significant between good (10%) and poor (71.42%) cytogenetic risk groups (P= 0.001), advanced stage MDS patients (66.66%) in comparison with early stage MDS patients (2.56%) (P= 0.00), higher- risk MDS group (61.53%) and lower- risk MDS group (7.31%) (P= 0.00). HRK hypermethylation was associated with advanced- stage MDS and downregulation of HRK gene may play a role in the progression of MDS.

متن کامل [PDF 207 kb]   (3565 دریافت)    
نوع مطالعه: Original Article | موضوع مقاله: Cancer
دریافت: 1395/1/21 | پذیرش: 1395/3/22 | انتشار: 1395/3/30

ارسال نظر درباره این مقاله : نام کاربری یا پست الکترونیک شما:
CAPTCHA

بازنشر اطلاعات
Creative Commons License این مقاله تحت شرایط Creative Commons Attribution-NonCommercial 4.0 International License قابل بازنشر است.

کلیه حقوق این وب سایت متعلق به International Journal of Molecular and Cellular Medicine IJMCM می باشد.

طراحی و برنامه نویسی : یکتاوب افزار شرق

© 2025 CC BY-NC 4.0 | International Journal of Molecular and Cellular Medicine IJMCM

Designed & Developed by : Yektaweb