2024-03-29T16:41:16+03:30 http://ijmcmed.org/browse.php?mag_id=37&slc_lang=en&sid=1
37-1227 2024-03-29 10.1002
International Journal of Molecular and Cellular Medicine (IJMCM) Int J Mol Cell Med 2251-9637 2251-9645 10.22088/IJMCM.BUMS 2020 9 2 Factors Influencing Mitochondrial Function as a Key Mediator of Glucose-Induced Insulin Release: Highlighting Nicotinamide Nucleotide Transhydrogenase Zahra Aghelan padidehbiochemist@yahoo.com Sara Kiani sarah.kiani.mbrc@gmail.com Abolfazl Nasiri Bionasiri69@gmail.com Masoud Sadeghi sadeghi_mbrc@yahoo.com Alireza Farrokhi alireza.farokhi@kums.ac.ir Reza Khodarahmi rkhodarahmi@mbrc.ac.ir Pancreatic β-cells recognize blood glucose changes and release insulin that is a peptide hormone responsible for stable glycemia. Diabetes, a chronic disorder of insulin insufficiency, leads to disturbed glucose homeostasis and multi-organ problems. Glucose and insulin are key markers in the follow-up and control of this disease. Mitochondrial metabolism of pancreatic beta cells is a crucial part of glucose-stimulated cascade of insulin secretion. Effective factors on β-cells mitochondrial function in production of compounds such as tricarboxylic acid intermediates, glutamate, nicotinamide adenine dinucleotide phosphate, and reactive oxygen species can have great effects on the secretion of insulin under diabetes. This review enhances our knowledge of factors influencing mitochondrial function as a key mediator of glucose-induced insulin release that accordingly will be helpful to further our understanding of the mechanisms implicated in the progressive beta cell failure that results in diabetes. Mitochondria glucose-sensing insulin release NADPH ROS diabetes 2020 6 01 107 121 http://ijmcmed.org/article-1-1227-en.pdf 10.22088/IJMCM.BUMS.9.2.107
37-1266 2024-03-29 10.1002
International Journal of Molecular and Cellular Medicine (IJMCM) Int J Mol Cell Med 2251-9637 2251-9645 10.22088/IJMCM.BUMS 2020 9 2 Increased Expression Level of Long Noncoding RNA H19 in Plasma of Patients with Myocardial Infarction Shokouh Safaei safaietolou@gmail.com Maryam Tahmasebi-Birgani maryam_tahmaseby@yahoo.com Mahdi Bijanzadeh mbijanz@yahoo.com Masoud Seyedian mseyedian@ajums.ac.ir Long noncoding RNAs (lncRNAs) are lengthy noncoding transcripts which are actively involved in crucial cellular pathways. Tissue-specific expression of lncRNAs besides its secretion into the body fluids, has made lncRNAs in attention as biomarkers of the diseases. According to the role of lncRNAs, especially H19 in cardiac regeneration, it is not surprising if their altered expression levels lead to cardiac diseases. In the present study, the relative expression of H19 was compared in the plasma of atherosclerotic myocardial infarction and control individuals by real time- PCR, and data were normalized using GAPDH. The association of plasma level of lipid and homocystine with H19 expression was also considered. The potential of H19 to discriminate the case from control was studied using the ROC analysis. We found that the plasma level of H19 transcript significantly increased in the plasma of patients in comparison with the control group. Additionally, the relative expression level of H19 was directly associated with the plasma homocystine level. The relative expression of H19 at threshold of 0.3 showed 70% sensitivity and 94% specificity to discriminate cases from controls. This study revealed that the expression level of H19 may be considered as a biomarker of myocardial infarction, although further studies are needed to generalize this finding. Atherosclerosis blood-based biomarker homocysteine long noncoding RNA myocardial infarction plasma 2020 6 01 122 129 http://ijmcmed.org/article-1-1266-en.pdf 10.22088/IJMCM.BUMS.9.2.122
37-1209 2024-03-29 10.1002
International Journal of Molecular and Cellular Medicine (IJMCM) Int J Mol Cell Med 2251-9637 2251-9645 10.22088/IJMCM.BUMS 2020 9 2 Evaluation of miRNAs Related with Nuclear Factor Kappa B Pathway in Lipopolysaccharide Induced Acute Respiratory Distress Syndrome Mustafa Azizoglu dryalamaoglu@hotmail.com Lokman Ayaz lokmanayaz@yahoo.com Gülsen Bayrak gulsenbayrak@mersin.edu.tr Banu Çoskun Yılmaz bcoskun@mersin.edu.tr Handan Birbiçer handanbirbicer@mersin.edu.tr Nurcan Doruk nurcandoruk@mersin.edu.tr This study aimed to determine the expression of nuclear factor kappa B (NF-κB) pathway related miRNAs in experimental acute respiratory distress syndrome (ARDS) induced by lipopolysaccharide (LPS) in rats, and to elucidate the underlying molecular mechanism. Twenty four sprague dawley rats were randomly divided into two groups; LPS (n = 12) and control (n = 12). Experimental ARDS was induced by intraperitoneal injection of E. coli LPS in LPS group. Intraperitoneal saline was administered in control group. Serum and lung samples were collected from both groups. Immunohistochemistry staining was performed for interleukin 1β (IL-1β), tumor necrosis factor α (TNF-α), CD 68, and caspase-3 in lung samples. Intensity of staining was scored as strong, moderate, weak, and no for evaluation of IL-1β and TNF-α. In addition, caspase-3 and CD 68 positive stained cells were counted in sections. Expressions of 9 miRNAs were determined by quantitative real-time PCR in serum samples.  IL-1β and TNF-α staining scores were significantly higher in the LPS group in comparison with the control group (P = 0.04 and P = 0.02, respectively). In addition, caspase-3 and CD 68 positive stained cells were significantly higher in the LPS group (P = 0.02). Expressions of seven miRNAs were significantly changed in the LPS group in comparison with the control group. While six miRNAs (miR-7a-5p, miR-7b, miR-9a-5p, miR-21-5p, miR-29a-3p and miR-138-5p) were up regulated, only miR-124-3p was down-regulated. This study suggests that these miRNAs may have a role in the pathogenesis of ARDS related to NF-κB. However, this relationship needs to be examined in new studies by evaluation of pathways and target genes. Acute respiratory distress syndrome ARDS microRNA nuclear factor kappa B 2020 6 01 130 139 http://ijmcmed.org/article-1-1209-en.pdf 10.22088/IJMCM.BUMS.9.2.130
37-1268 2024-03-29 10.1002
International Journal of Molecular and Cellular Medicine (IJMCM) Int J Mol Cell Med 2251-9637 2251-9645 10.22088/IJMCM.BUMS 2020 9 2 Dynamic of miRNA-101a-3p and miRNA-200a during Induction of Osteoblast Differentiation in Adipose-derived Mesenchymal Stem Cells Somayeh Aslani smh.aslani@gmail Reza Rahbarghazi rezarahbardvm@gmail.com Sevda Rahimzadeh rahimzadeh.sevda@gmsil.com Hadi Rajabi hadirajabi.bio@gmail.com Alireza Abhari abharial@tbzmed.ac.ir Ebrahim Sakhinia esakhinia@yahoo.co.uk MiRNAs are known as the cellular phenomena regulators that exert their effects in post-transcriptional level. Recent studies highlight the role of miRNAs in mesenchymal stem cells differentiation into osteoblasts. The purpose of this study was to recognize the pattern of miRNA-101a-3p and miRNA-200a expression during osteoblastic differentiation of human adipose tissue-derived mesenchymal stem cells. The cells were incubated in osteoblastic differentiation medium for a period of 21 days. Alizarin red S staining was performed to confirm the successful differentiation of adipose-derived mesenchymal stem cells into osteoblast cells. The expression levels of miRNA-101a-3p and miRNA-200a were analyzed by real-time PCR during 0, 7, 14 and 21 days after differentiation induction. Data exhibited the increase of extracellular red color deposition which was evident at the end of the incubation period. The expression of miRNA-101a-3p and miRNA-200a was upregulated during adipose-derived mesenchymal stem cells trans-differentiation into osteoblast-like cells. These miRNAs could be potential novel biomarkers for monitoring successful differentiation of mesenchymal stem cells toward osteoblasts.   Adipose-derived-mesenchymal stem cells osteoblast-like differentiation miRNA-101a-3p miRNA-200a transcription 2020 6 01 140 145 http://ijmcmed.org/article-1-1268-en.pdf 10.22088/IJMCM.BUMS.9.2.140
37-1154 2024-03-29 10.1002
International Journal of Molecular and Cellular Medicine (IJMCM) Int J Mol Cell Med 2251-9637 2251-9645 10.22088/IJMCM.BUMS 2020 9 2 HSV-TK Expressing Mesenchymal Stem Cells Exert Inhibitory Effect on Cervical Cancer Model Azra Kenarkoohi a.Kenarkoohi@gmail.com Taravat Bamdad bamdad_t@modares.ac.ir Masoud Soleimani soleim_m@modares.ac.ir Hoorieh Soleimanjahi soleim_h@modares.ac.ir Ali Fallah ali@rnax.co.uk Shahab Falahi shahabivan@gmail.com A growing area of research is focused on cancer therapy, and new therapeutic approaches are welcomed. Mesenchymal stem cell (MSC)-based gene therapy is a promising strategy in oncology. Intrinsic tropism and migration to tumor microenvironment with off lights are attractive features of this type of cell carrier. In this way, suicide genes have also found a good platform for better performance and have shown a stronger anti-tumor mechanism by riding on mesenchymal cells. In this study, we investigated the anti-tumor activity of intratumoral injected MSCs transduced with a lentivector expressing the HSV/TK in a mouse cervical cancer model. Following the injection of MSCs transduced with lentivector carrying TK, MSCs alone or PBS into the mice tumor, ganciclovir was administered intraperitoneally during 14 days, and tumor size, survival time, natural killer (NK) cells and cytotoxic T lymphocyte (CTL) activities were assessed. We demonstrated that combination of suicide therapy and cell therapy leading m,to successful tumor inhibition. Significant reduction in tumor size was detected in test group in comparison with controls. Also, potent antitumor NK and CTL activity was seen in treatment group in comparison with controls. Our data demonstrated that the mesenchymal cells expressing TK had inhibitory effect on cervical cancer model. Cervical cancer mesenchymal stem cell suicide therapy lentivector thymidine kinase cell therapy 2020 6 01 146 153 http://ijmcmed.org/article-1-1154-en.pdf 10.22088/IJMCM.BUMS.9.2.146
37-1317 2024-03-29 10.1002
International Journal of Molecular and Cellular Medicine (IJMCM) Int J Mol Cell Med 2251-9637 2251-9645 10.22088/IJMCM.BUMS 2020 9 2 Relationship between Single Nucleotide Polymorphisms of GRHL3 and Schizophrenia Susceptibility: A Preliminary Case-Control Study and Bioinformatics Analysis Saman Sargazi sgz.biomed@gmail.com Milad Heidari Nia milad_heidari2003@yahoo.com Roghayeh Sheervalilou r_valiloo@yahoo.com Shekoufeh Mirinejad shokufemn@gmail.com Mahdiyeh Harati-Sadegh m.haratisadegh@yahoo.com Mahdiyeh Moudi mahdiyehmodi@yahoo.com Ramin Saravani saravaniramin@yahoo.com Mansoor Shakiba mans30817@gmail.com Grainyhead-like (GRHL) transcription factors were recently linked to the etiology of neural tube defects (NTDs). Overlapping patterns in the variation of schizophrenia (SCZ) incidence with that of NTDs suggests the presence of common etiological risk factors. This preliminary study was designed to examine the relationship between two missense variants of GRHL3 gene (rs2486668C/G and rs545809A/T) and SCZ susceptibility among Iranians. Three hundred ninety subjects (192 patients confirmed with SCZ, and 198 healthy controls) were enrolled and genotyped. Statistical and bioinformatics analyzes were performed to determine the effects of the variants. In silico analyzes were performed to determine the effects of the variants on the secondary structure of GRHL3 protein and prediction of silencer motifs for each variation. Statistically significant differences were observed between the studied groups under codominant AA, dominant AT+AA, and recessive AA genetic contrast models for rs545809A/T. The presence of the A allele of rs545809A/T enhanced SCZ risk by 2.33 fold. In contrast, rs2486668C/G was not linked to SCZ susceptibility (P > 0.05). Bioinformatics analysis revealed that both missense SNPs caused substantial changes in the secondary structure of GRHL3-mRNA. Screening of the flanking sequences of rs545809A/T predicted silencer motifs for this SNP. Our results demonstrated that the rs545809A/T of GRHL3 gene could affect the risk of SCZ in Iranian populations. Replication studies are warranted to confirm these results. Bioinformatics grainyhead-like 3 haplotype polymorphism schizophrenia 2020 6 01 154 164 http://ijmcmed.org/article-1-1317-en.pdf 10.22088/IJMCM.BUMS.9.2.154
37-1277 2024-03-29 10.1002
International Journal of Molecular and Cellular Medicine (IJMCM) Int J Mol Cell Med 2251-9637 2251-9645 10.22088/IJMCM.BUMS 2020 9 2 Comprehensive Analysis of Zinc Derivatives Pro-proliferative, Anti-Apoptotic and Antimicrobial Effect on Human Fibroblasts and Keratinocytes in a Simulated, Nutrient-deficient Environment In Vitro Julian-Dario Rembe julian-dario.rembe@uni-wh.de Julia Katharina Boehm julia.boehm@uni-wh.de Carolin Fromm-Dornieden carolinfromm@gmx.de Nina Hauer nina.hauer@uni-wh.de Ewa Klara Stuermer e.stuermer@uke.de Zinc as therapeutic agent in skin and wound care has been known for centuries, but its role is controversial and comprehensive investigations in nutrient-deficient environments are lacking. We aimed to provide a broad analysis of different zinc derivatives on proliferation, apoptosis and antimicrobial properties in a simulated nutrient-deficient environment in vitro. Human fibroblasts (CRL2522) and keratinocytes (HaCaT) were treated with a broad concentration range (10 – 0.0001 µg/mL) of zinc-sulfate (ZnSO4), -gluconate (ZnGluc) and -histidine (ZnHis) for 1-6 days under nutrient-deficient media conditions. Cell proliferation was investigated by XTT assay. Targeted analyzes in proliferation (E2F1, PCNA) and apoptosis (TP53) associated genes were performed via qRT-PCR and apoptosis was determined via FACS (annexin V/7-AAD staining). Antimicrobial efficacy was investigated using a quantitative suspension method against S. aureus, P. aeruginosa, E. coli, and C. albicans. The results indicated that 0.1 to 0.001 µg/mL Zn increased cell proliferation in both cell lines. Fibroblasts were more susceptible with significant proliferation peaks on days 2 & 6, and days 1 & 4 for keratinocytes. No relevant changes in gene expression were detected for E2F1 and PCNA nor for TP53. Annexin-V/7-AAD-staining of fibroblasts revealed a small, yet insignificant reduction of apoptosis induction for ZnGluc and ZnSO4. ZnGluc and ZnSO4 (0.1%) achieved high microbial reductions (4-5 log10 reductions) against tested pathogens. ZnGluc and ZnSO4 showed relevant pro-proliferative and antimicrobial, as well as tendential anti-apoptotic features in a simulated nutrient-deficient microenvironment in vitro. This further validates a potential benefit of local zinc treatment in deficient wound microenvironments. Wound healing zinc fibroblasts keratinocytes antimicrobial efficacy cell proliferation apoptosis 2020 6 01 165 179 http://ijmcmed.org/article-1-1277-en.pdf 10.22088/IJMCM.BUMS.9.2.165