2024-03-29T01:38:26+03:30 http://ijmcmed.org/browse.php?mag_id=28&slc_lang=en&sid=1
28-836 2024-03-29 10.1002
International Journal of Molecular and Cellular Medicine (IJMCM) Int J Mol Cell Med 2251-9637 2251-9645 10.22088/IJMCM.BUMS 2018 7 2 Derived Mesenchymal Stem Cells in Addiction Related Hippocampal Damages Raheleh Rafaiee Naghmeh Ahmadiankia Ahmadian@shmu.ac.ir The brain is an important organ that controls all sensory and motor actions, memory, and emotions. Each anatomical and physiological modulation in various brain centers, results in psychological, behavioral, and sensory-motor changes. Alcohol and addictive drugs such as opioids and amphetamines have been shown to exert a great impact on brain, specifically on the hippocampus. Emerging evidence has indicated that altered hippocampal neurogenesis is associated with the pathophysiology of neuropsychological disorders including addiction. The addictive drugs impair neurogenesis and undermine the function of neural stem/progenitor cells in hippocampus. This feature was claimed to be one of the underlying mechanisms of behavioral changes in patients with addiction. As the impairment of stem cells’ function has been proved to be the underlying cause of pathologic neuroadaptations in the brain, the administration of stem cell populations has shown promising results for re-modulating of neuronal status in the brain and especially in the hippocampus. Among different types of stem cells, bone marrow derived mesenchymal stem cells are the most proper candidates for stem cell therapies. In this review article, the recent studies on the effects of addictive drugs on brain neurogenesis, and also the promising potential effects of stem cells in curing addiction related hippocampal damages are discussed. addiction hippocampus neurogenesis neural stem/progenitor cells mesenchymal stem cells 2018 5 01 69 79 http://ijmcmed.org/article-1-836-en.pdf 10.22088/IJMCM.BUMS.7.2.69
28-826 2024-03-29 10.1002
International Journal of Molecular and Cellular Medicine (IJMCM) Int J Mol Cell Med 2251-9637 2251-9645 10.22088/IJMCM.BUMS 2018 7 2 Hydroxyapatite from Fish for Bone Tissue Engineering: A Promising Approach Renata Neves Granito re_neves@yahoo.com.br Ana Claudia Renno a.renno@unifesp.br Hirochi Yamamura yhirochi@uol.com.br Matheus Cruz de Almeida ak92@hotmail.com Pedro Luiz Menin Ruiz pedrolmruiz@hotmail.com Daniel Araki Ribeiro daribeiro@unifesp.br Natural or synthetic hydroxyapatite (HA) has been frequently used as implant materials for orthopaedic and dental applications, showing excellent bioactivity, adequate mechanical rigidity and structure, osteoconductivity and angiogenic properties, no toxicity, and absence of inflammatory or antigenic reactions. HA can be easily synthesized or extracted from natural sources, such as bovine bone. However, the manufacturing costs to obtain HA are high, restricting the therapy. Herein, much effort has been paid for obtaning alternative natural sources for HA. The potential of HA extracted from skeleton of animals has been investigated. The aim of this review is to exploit the potential of HA derived from fish to fulfill biological activities for bone tissue engineering. In particular, HA from fish is easy to be manufactured regarding that the majority of protocols are based on the calcination method. Furthermore, the composition and structure of HA from fish were evaluated; the biomaterial showed good biocompatibility as a result of non-cytotoxicity and handling properties, demonstrating advantages in comparison with synthetic ones. Interestingly, another huge benefit brought by HA from bone fish is its positive effect for environment since this technique considerably reduces waste. Certainly, the process of transforming fish into HA is an environmentally friendly process and stands as a good chance for reducing costs of treatment in bone repair or replacement with little impact into the environment. hydroxyapatite review fish waste tissue engeneering 2018 5 01 80 90 http://ijmcmed.org/article-1-826-en.pdf 10.22088/IJMCM.BUMS.7.2.80
28-843 2024-03-29 10.1002
International Journal of Molecular and Cellular Medicine (IJMCM) Int J Mol Cell Med 2251-9637 2251-9645 10.22088/IJMCM.BUMS 2018 7 2 Potential Use of Amniotic Membrane Derived Scaffold for Cerebrospinal Fluid Applications Fereshteh Dorazehi banady69@gmail.com Mohammad Nabiuni devbiokharazmi@gmail.com Hanieh Jalali jalalidev@gmail.com Scaffolds derived from decellularized tissues provide a natural microenvironment for cell culture. Embryonic cerebrospinal fluid (e-CSF) contains factors which play vital roles in development of the nervous system. This research was aimed to survey the effect of Wistar rat e-CSF on neural differentiation of bone marrow derived mesenchymal stem cells (BM-MSCs) cultured on the human amniotic membrane (AM). BM-MSCs were collected from femurs and tibias, and were cultured in Dulbecco's Modified Eagle's Medium. The placenta was harvested from healthy women during cesarean section and AM was acellularized using EDTA and physical scrubbing. e-CSF was harvested from rat fetuses at E17. Adequate numbers of BM-MSCs were cultured on acellularized membrane, and were treated with E17 CSF for 7 days. MTT (3-(4, 5-dimethylthiazol-2-yl)-2.5-diphenyltetrazolium bromide) assay confirmed the survival and proliferation of BM-MSCs cultured on AM derived scaffold. Hematoxylin/eosin staining and scanning electron microscopy showed the morphological and the structural changes of BM-MSCs throughout the culture and treatment with e-CSF. The results of immunocytochemistry showed that microtubule associated protein 2 and beta-III tubulin were expressed in BM-MSCs cultured on acellular amnion scaffold and treated with e-CSF. Our results showed for the first time that the combination of acellular AM as a natural scaffold and e-CSF as a source of neurological factors could effectively improve the BM-MSCs cultivation and differentiation. Cerebrospinal Fluid Bone Marrow Mesenchymal Stem Cells Neural Differentiation Amnion Membrane Derived Scaffold 2018 5 01 91 101 http://ijmcmed.org/article-1-843-en.pdf 10.22088/IJMCM.BUMS.7.2.91
28-821 2024-03-29 10.1002
International Journal of Molecular and Cellular Medicine (IJMCM) Int J Mol Cell Med 2251-9637 2251-9645 10.22088/IJMCM.BUMS 2018 7 2 The Growth Arrest-Specific Transcript 5 (GAS5) and Nuclear Receptor Subfamily 3 Group C Member 1 (NR3C1): Novel Markers Involved in Multiple Sclerosis Jalal Gharesouran Gharesouranj@tbzmed.ac.ir Mohammad Taheri mohammad_823@yahoo.com Arezou Sayad ar.sayad@yahoo.com Soudeh Ghafouri-Fard ghafourifard@razi.tums.ac.ir Mehrdokht Mazdeh Mazdeh.m@yahoo.com Mir Davood Omrani davood_omrani@yahoo.co.uk Recent studies have revealed that long non-coding RNAs (lncRNAs) are connected with pathogenesis of neurodegenerative diseases. Additionally, glucocorticoids have fundamental regulatory roles on the immune system, and act as potent therapeutic compounds for autoimmune and inflammatory diseases. The long noncoding RNA growth arrest-specific 5 (GAS5) which accumulates inside the cells in response to cellular starvation/growth arrest, acts as a potent repressor of the glucocorticoid receptor (GR) through its glucocorticoid response element (GRE). The aim of the present study was to investigate the role of lncRNA GAS5 and its downstream target Nuclear Receptor Subfamily 3 Group C Member 1(NR3C1) in the pathogenesis of multiple sclerosis (MS), and to define the role of GAS5 in the regulation of NR3C1 expression. Quantitative polymerase chain reaction was performed for investigating the expression of GAS5 and NR3C1 in MS patients and healthy subjects. We found that GAS5 levels were up-regulated in blood of MS patients compared with healthy subjects in correlation with NR3C1 expression. Our findings suggest that GAS5 may play important role in the molecular etiology and treatment of MS. Multiple sclerosis GAS5 NR3C1 2018 5 01 102 110 http://ijmcmed.org/article-1-821-en.pdf 10.22088/IJMCM.BUMS.7.2.102
28-839 2024-03-29 10.1002
International Journal of Molecular and Cellular Medicine (IJMCM) Int J Mol Cell Med 2251-9637 2251-9645 10.22088/IJMCM.BUMS 2018 7 2 Circulatory YKL-40 & NLR: Underestimated Prognostic Indicators in Diffuse Glioma Puneet Gandhi puneetgandhi67@yahoo.com Richa Khare richakhare24@gmail.com Hanni Gulwani hanni.gulwani@yahoo.com Sukhpreet Kaur drsukh.kaur@yahoo.co.in In addition to histopathological parameters, evaluation of associated hematological factors is essential for devising a sensitive prognostic scale in glioma. Increased neutrophil-lymphocyte ratio (NLR), a marker of systemic inflammatory response, has recently been associated with worse outcome in various cancers. Given that glioma progression is characterized by inflammation, aggressive angiogenesis, and invasion, increased levels of systemic human-chitinase-3-like-one protein (YKL-40) have also been linked to poor prognosis. The aim of the present study was to assess the plausible association of YKL-40, NLR, and platelet count with increasing tumor grade, and evaluate their status as independent prognostic factors in terms of overall survival (OS) in therapy naive patients with diffuse glioma. Plasma levels of both biochemical markers in 72 diffuse gliomas, median age 42 years, were compared with 36 controls. Comparison of YKL-40, NLR, and PC with respect to tumor grade was found to be significant for each of the markers (P < 0.0001) while an inverse significant correlation was seen for YKL-40 and NLR with OS (r = -0.4619, P < 0.0001, and r = -0.5561, P < 0.0001, respectively). NLR was the best performing marker with AUC 0.9417 at 97% specificity. In addition, YKL-40 had a positive correlation with NLR (r = 0.4902, P < 0.0001), indicating that expression of both markers was linked to inflammation and tumor progression as they were significantly correlated with tumor grade. Expression of YKL-40 and NLR was independently associated with worse survival (HR 1.0062, P = 0.039, and HR 1.1787, P = 0.0003, respectively), thus establishing their clinical utility as prognosticators for diffuse gliomas. Diffuse glioma YKL-40 neutrophil-lymphocyte ratio overall survival prognostic marker tumor progression 2018 5 01 111 118 http://ijmcmed.org/article-1-839-en.pdf 10.22088/IJMCM.BUMS.7.2.111
28-885 2024-03-29 10.1002
International Journal of Molecular and Cellular Medicine (IJMCM) Int J Mol Cell Med 2251-9637 2251-9645 10.22088/IJMCM.BUMS 2018 7 2 Dysregulated Expression and Sub cellular Localization of Base Excision Repair (BER) Pathway Enzymes in Gallbladder Cancer Manoj Kumar manoj@shooliniuniversity.com Vijay Kumar Shukla vkshuklabhu@gmail.com Pravas Kumar Misra rashmipravas080@gmail.com Mercy Jacob Raman mercyjraman@gmail.com Base excision repair (BER) pathway is one of the repair systems that have an impact on the radiotherapy and chemotherapy for the cancer patients. The molecular pathogenesis of gallbladder cancer is not known extensively. In the present study we investigated whether the expression of AP endonuclease 1 (APE1) and DNA polymerase β (DNA pol β), key enzymes of BER pathway has any clinical significance with gallbladder carcinogenesis. 41 gallbladder cancer, 27 chronic cholecystitis, and 3 normal gallbladder specimens were analyzed for the expression of APE1 and DNA polymerase β by western blotting, and sub cellular localization were studied by immunohistochemistry. The enzymatic activity of APE1 was also studied. The correlations with expression of the above proteins with clinical-pathological characteristics of gallbladder cancer patients were analyzed. The integrated density value ratio (relative expression) of total APE1 (37 kDa + 35 kDa variant) analyzed in the three groups of tissues, were 0.76±0.03 in normal gallbladder, 0.91±0.08 in chronic cholecystitis, and 1.12±0.05 in gallbladder cancer. APE1 was found to be up-regulated in 80% of gallbladder carcinoma samples (P = 0.01). A positive trend of APE1 expression with tumor stage and lymph node positivity was observed. The enzymatic activity of APE1 was found higher in gallbladder cancer samples in comparison with chronic cholecystitis. The integrated density value  ratio of DNA polymerase β for normal gallbladder, chronic cholecystitis and gallbladder cancer tissue samples were 0.46±0.03, 0.7±0.06 and 1.33±0.1, respectively. DNA polymerase β was found to be up regulated in almost all gallbladder carcinoma samples (P = 0.0001), and its expression was negatively correlated with age (P = 0.02). DNA polymerase β expression showed a positive trend with tumor stage and nuclear differentiation of gallbladder cancer. . It may be concluded that alteration of these BER pathway proteins may be the causal factors for carcinogenesis of gallbladder, and has targeted therapeutic potential. AP endonuclease1 DNA polymerase β chronic cholecystitis gallbladder cancer. 2018 5 01 119 132 http://ijmcmed.org/article-1-885-en.pdf 10.22088/IJMCM.BUMS.7.2.119
28-820 2024-03-29 10.1002
International Journal of Molecular and Cellular Medicine (IJMCM) Int J Mol Cell Med 2251-9637 2251-9645 10.22088/IJMCM.BUMS 2018 7 2 Screening for Non-polyenic Antifungal Produced by Actinobacteria from Moroccan Habitats: Assessment of Antimycin A19 Production by Streptomyces albidoflavus AS25 Ahmed Nafis ahmed.nafis@edu.uca.ac.ma Najoua Elhidar najoua.elhidar@gmail.com Brahim Oubaha oubaha123@gmail.com Salah eddine Samri salahsamri@gmail.com Timo Niedermeyer timo.niedermeyer@pharmazie.unihalle.de Yedir Ouhdouch youhdouch@gmail.com Lahcen Hassani lhassani@uca.ac.ma Mustapha Barakate Barakate barakate@uca.ma Fungal diseases are currently a serious public health problem, due to the limited number of really effective principles, and the emergence of resistant strains to the polyenic antifungals. The aim of this study was to screen, for non-polyenic antifungals production by Actinobacteria, and to validate the screening program by characterizing the produced compounds. Actinobacteria isolates were tested against four clinic human pathogenic fungi isolated from Hospital Mohammed V Rabat, Morocco. The production of non-polyenic antifungal metabolites by active isolates was investigated based on the yeast cell specificity as challenging targets, antibacterial activity, activity against resistant Candida tropicalis R2 and Pythium irregular (resistant to polyenes), inhibition of antifungal activity by the addition of exogenous ergosterol, and the UV-visible light spectrophotometric analysis of the active crude extracts. The antifungal compound produced was purified using various chromatographic techniques and the selected producing strain was identified using the polyphasic approach. Among 480 Actinobacteria isolates, 55 showed antifungal activity against all tested clinically derived fungi. After performing the screening program, 4 Actinobacteria that had all the desired criteria were selected. Using the polyphasic approach, the taxonomic position of the selected  Streptomyces AS25, isolated from rhizospheric soil of Alyssum spinosum, showed that it belongs to Streptomyces genus with 100% partial 16S similarity with Streptomyces albidoflavus NBRC13010. On the basis of HPLC and mass spectrometry, the purified compound produced by Streptomyces AS25 was identified as a non-polyenic lactone, antimycin A19, which has been found for the first time to be produced by Streptomyces albidoflavus strain. Following the obtained results, it is important to note that our screening criteria for non-polyenic antifungals have been validated and the rhizospheric soil represent an interesting source to isolate Actinobacteria. Validation program screening non-polyenic antifungal Actinomycetes characterization Antimycin A19 2018 5 01 133 145 http://ijmcmed.org/article-1-820-en.pdf 10.22088/IJMCM.BUMS.7.2.133