Babol University of Medical Sciences
International Journal of Molecular and Cellular Medicine (IJMCM)
2251-9637
2
1
2013
3
1
Polymorphism of Genes and Implantation Failure
1
8
EN
majid
Mojarad
Department Medical Genetics, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
Mohammad
Hassanzadeh-Nazarabad
Medical Genetics Research Center, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
Niaiesh
Tafazoli
Department Medical Genetics, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
Implantation failure is the most frequent cause of pregnancy loss in couples who try to conceive, either in a natural way or using assisted reproductive techniques (ART). Identify the precise mechanisms of implantation failure can lead to identify couples at risk and also providing appropriate therapeutic options to affected couples. Despite the high prevalence of this disorder, a few causing factors are demonstrated so far. Recent studies indicate that genetic factors play an important role in the occurrence of recurrent implantation failure. Although some of these factors, such as numerical chromosomal aneuploidy are known to be causative factors, there are some other factors that solely increase susceptibility to this event. In the present review we try to list the genetic polymorphisms that are known as susceptibility factors in implantation failure.
Babol University of Medical Sciences
International Journal of Molecular and Cellular Medicine (IJMCM)
2251-9637
2
1
2013
3
1
Determination of ctxAB expression in Vibrio cholerae Classical and El Tor strains using Real-Time PCR
9
13
EN
Seyed Mahmoud
Amin Marashi
Cellular and Molecular Biology Research Center (CMBRC), Babol University of Medical Sciences, Babol, Iran.
Ramazan
Rajabnia
Infectious Diseases & Tropical Medicine Research Center, Department of Microbiology and Immunology, Babol University of Medical Sciences, Babol, Iran.
Abbas Ali
Imani Fooladi
Applied Microbiology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran.
Zohreh
Hojati
Genetics Division, Biology Department, Faculty of Sciences, University of Isfahan, Isfahan, Iran.
Sharareh
Moghim
Department of Microbiology, Isfahan University of Medical Sciences, Isfahan, Iran.
Bahram
Nasr Esfahani
Department of Microbiology, Isfahan University of Medical Sciences, Isfahan, Iran.
Cholera is an infection of the small intestines caused by the bacterium V. cholerae. It is a major cause of health threat and also a major cause of death worldwide and especially in developing countries. The major virulence factor produced by V. cholerae during infection is the cholera toxin. Total mRNA extraction and reverse transcription was performed for making ctxAB cDNA. Relative Real-Time PCR analysis showed unequal enterotoxin production in V. cholerae strains. The results showed that, classical strain produces cholera toxin more than El Tor strain.
Babol University of Medical Sciences
International Journal of Molecular and Cellular Medicine (IJMCM)
2251-9637
2
1
2013
3
1
Comparison of molecular mutations of G6PD deficiency gene between icteric and nonicteric neonates
14
20
EN
Yadollah
Zahedpasha
Non-Communicable Pediatric Diseases Research Center, Babol University of Medical Sciences, Babol, Iran.
Mousa
Ahmadpour Kachouri
Non-Communicable Pediatric Diseases Research Center, Babol University of Medical Sciences, Babol, Iran.
Haleh
Akhavan Niaki
Cellular and Molecular Biology Research Center (CMBRC), Babol University of Medical Sciences, Babol, Iran.
Roya
Farhadi
Non-Communicable Pediatric Diseases Research Center, Babol University of Medical Sciences, Babol, Iran.
Background and Aim: Jaundice is a common disorder in neonates and one of the provable causes of glucose-6-phosphate dehydrogenase (G6PD) deficiency, some mutation types of which may be associated with severe neonatal icter. In this line, the present study has been conducted to compare G6PD mutations in incteric and non icteric neonates.
Materials and Methods: This case-control study was implemented in the NICU and Newborn Ward of Amirkola Children Hospital in 2007-2008. Available sampling approach was used and 50 icteric as well as 50 non-icteric newborns, both with G6PD deficiency, were selected as the case and the control group respectively. Two milliliters of peripheral blood were collected in EDTA-containing tubes after parental consent and G6PD deficiency was diagnosed using FST (Fluorescent Spot Test) method. All samples were first evaluated in terms of Mediterranean mutation and the negative cases of this mutation were then examined for Chatham mutation all remaining samples were finally tested for Cosenza mutation. After the completion of the above steps, G6PD mutations were compared in the two groups and P-value less than 0.05 was considered significant.
Results: In icteric group, 76% were male and 24% were female and in non-icteric group, 70% were male and 30% were female. The mean weight of neonates was 3.2 ± 0.4 kg and 2.8 ± 0.8 kg in icteric and non-icteric groups respectively (p0.05), however, the distribution of rare mutations (Cosenza negative) was significantly different between icteric and non-icteric groups with enzyme deficiency (p 0.05). Newborns with Chatham mutation have been less in need of exchange transfusions (p <0.05).
Conclusions: Findings of the present study showed that there was no significant relationship between prevalent mutations of G6PD gene and the incidence of icter in Mediterranean and Chatham mutations Mediterranean mutation was the most common mutation in the mentioned gene in icteric and non-icteric neonates in our region, while Cosenza mutation was more frequent in non-icteric group, indicating that rare mutations of G6PD gene may less likely lead to neonatal icter. Furthermore, the need for exchange transfusion is less in icteric neonates with Chatham mutation which is indicative of milder clinical phenotype in this type of mutation.
Babol University of Medical Sciences
International Journal of Molecular and Cellular Medicine (IJMCM)
2251-9637
2
1
2013
3
1
Effect of Urtica dioica Leaf Alcoholic and Aqueous extracts on the Number and the Diameter of the Islets in Diabetic Rats
21
26
EN
Durdi
Qujeq
Cellular and Molecular Biology Research Center (CMBRC), Babol University of Medical Sciences, Babol, Iran.
Mohsen
Tatar
Department of Biochemistry and Biophysics, Faculty of Medicine, Babol University of Medical Sciences, Babol, Iran.
Farideh
Feizi
Department of Anatomical Sciences, Faculty of Medicine, Babol University of Medical Sciences, Babol, Iran.
Hadi
Parsian
Cellular and Molecular Biology Research Center (CMBRC), Babol University of Medical Sciences, Babol, Iran.
Alieh Sohan
Faraji
Department of Anatomical Sciences, Faculty of Medicine, Babol University of Medical Sciences, Babol, Iran.
Sohrab
Halalkhor
Cellular and Molecular Biology Research Center (CMBRC), Babol University of Medical Sciences, Babol, Iran.
Preliminary studies revealed that Urtica dioica has been known as a plant that decrease blood glucose. Despite the importance of this plant in herbal medicine, relatively little research has been down on effects of this plant on islets yet. The objective of the current study was to evaluate the effect of dried urtica dioica leaf alcoholic and aqueous extracts on the number and the diameter of the islets and histological parameters in streptozocin-induced diabetic rats. Six rats were used in each group. Group I : Normal rats were administered saline daily for 8 weeks . Group II : Diabetic rats were administered streptozotocin, 50 mg/kg of body weight Group III : Diabetic rats were administered dried Urtica Dioica leaf aqueous extracts for 8 weeks Group IV: Diabetic rats were administered dried Urtica Dioica leaf alcoholic extracts for 8 weeks . The animals, groups of diabetic and normal, were sacrificed by ether anaesthesia. Whole pancreas was dissected. The tissue samples were formalin fixed and paraffin embedded for microscopic examination. Histologic examination and grading were carried out on hematoxylin-eosin stained sections. The effects of administration of dried Urtica Dioica leaf alcoholic and aqueous extracts to diabetic rats were determined by histopathologic examination. The pancreas from control rats showed normal pancreatic islets histoarchitecture. Our results also, indicate that the pancreas from diabetic rats show injury of pancreas tissue while the pancreas from diabetic rats treated with dried Urtica Dioica leaf alcoholic and aqueous extracts show slight to moderate rearrangement of islets. According to our findings, dried urtica dioica leaf alcoholic and aqueous extracts can cause a suitable repair of pancreatic tissue in streptozocin-induced diabetic experimental model.
Babol University of Medical Sciences
International Journal of Molecular and Cellular Medicine (IJMCM)
2251-9637
2
1
2013
3
1
A comparative study of PCNA and Ki-67 expression in dental follicle, dentigerous cyst, unicystic ameloblastoma and ameloblastoma
27
33
EN
Shima
Nafarzadeh
Dental material research center, Babol University of Medical Sciences, Babol, Iran.
Maryam
Seyedmajidi
Department of Oral and Maxillofacial Pathology, Dentistry school, Babol University of Medical Sciences, Babol, Iran.
Sina
Jafari
Student Research Committee, Babol University of Medical Sciences, Babol, Iran.
Ali
Bijani
Non-communicable Pediatric Diseases Research Center, Babol University of Medical Sciences, Babol, Iran.
Ali
Rostami Sarokolaei
Dentistry school, Babol University of Medical Sciences, Babol, Iran.
Various cell proliferation markers are used as diagnostic and prognostic tools in oral lesions. Simultaneous evaluation of these markers can increase the precision of estimation of the proliferative status of different tissues.
In this study we investigated the expression of PCNA and Ki-67 as markers of cell proliferation in 15 paraffin embedded samples of each dental follicle, dentigerous cyst, unicystic ameloblastoma and ameloblastoma belonging to a total of 30 male and 30 female paients using immunohistochemistry method. Expression levels based on the intensity and the percentage of stained cells was separately analyzed for each marker with chi-square test, the results of which were significant for the two markers (P<0.05). The correlation coefficient between the two markers was found to be 0.88. A significant difference in the expression of Ki-67 and PCNA was observed in the four types of studied lesions.
Babol University of Medical Sciences
International Journal of Molecular and Cellular Medicine (IJMCM)
2251-9637
2
1
2013
3
1
VCAM1 and ICAM1 expression in oral lichen planus
34
40
EN
Maryam
Seyedmajidi
Dental Materials Research Center, Dental Faculty, Babol University of Medical Sciences, Babol, Iran.
Shahryar
Shafaee
Cellular & Molecular Biology Research Center, Babol University of Medical Sciences, Babol, Iran.
Ali
Bijani
Non-communicable Pediatrics Diseases Research Center, Babol University of Medical Sciences, Babol, Iran.
Soodabeh
Bagheri Moghaddam
Students Research Committee, Babol University of Medical Sciences, Babol, Iran.
Oral lichen planus is a chronic inflammatory immune-mediated disease. ICAM-1 and VCAM-1 are vascular adhesion molecules that their receptors are located on endothelial cells and leukocytes. The aim of this study is the immunohistochemical evaluation of VCAM1 and ICAM1 in oral lichen planus and to compare these two markers with normal mucosa for evaluation of angiogenesis. This descriptive-analytical study was performed on 70 paraffined blocks of oral lichen planus and 30 normal mucosa samples taken from around the lesions. Samples were stained with H & E and then with Immunohistochemistry using monoclonal mouse anti human VCAM1 (CD106), & monoclonal mouse anti human ICAM1(CD54) for confirmation of diagnosis. Slides were evaluated under light microscope and VCAM1 and ICAM1 positive cells (endothelial cells and leukocytes) were counted. Data were analyzed with Mann-Whitney test, Wilcoxon and Chi-Square and p<0.001 was declared significant.
VCAM1 and ICAM1 expression significantly increased compared to normal mucosa in oral lichen planus according to the percentage of stained cells (p=0.000; p=0.000, Mann-Whitney test). Thirty cases of oral normal mucosa associated with lichen planus showed that the VCAM1 has increased significantly in comparison to normal mucosa (p<0.001). Also, ICAM1 expression between lichen planus and normal mucosa, showed a significantly difference (p0.05). Regarding the results, it seems that high expression of VCAM1 and ICAM1 is related to oral lichen planus.
Babol University of Medical Sciences
International Journal of Molecular and Cellular Medicine (IJMCM)
2251-9637
2
1
2013
3
1
Investigation of LRTOMT gene (locus DFNB63) mutations in Iranian patients with autosomal recessive non-syndromic hearing loss
41
45
EN
Seyyed Hossein
Taghizadeh
Department of Genetic, Faculty of Science, ShahidChamran University of Ahwaz, Ahwaz, Iran.
Seyyed Reza
Kazeminezhad
Department of Genetic, Faculty of Science, ShahidChamran University of Ahwaz, Ahwaz, Iran.
Seyyed Ali Asghar
Sefidgar
Cellular and Molecular Biology Research Center (CMBRC), Babol University of Medical Sciences, Babol, Iran.
Nasrin
Yazdanpanahi
Department of Biochemistry and Genetics, Falavarjan Branch, Islamic Azad University, Isfahan, Iran.
Mohammad Amin
Tabatabaeifar
Department of Genetics, Faculty of Medicine, JundiShapur University of Medical Sciences, Ahwaz, Iran.
Ahmad
Yousefi
Department of Basic Sciences, Faculty of Veterinary Medicine, Shahrekord University, Shahrekord, Iran.
Seyyed Mohammad
Lesani
Department of Genetics, Faculty of Science, Shahrekord University, Shahrekord, Iran.
Marziyeh
Abolhasani
Cellular and Molecular Research Center, Shahrekord University of Medical Sciences, Shahrekord, Iran.
Morteza
Hashemzadeh Chaleshtori
Cellular and Molecular Research Center, Shahrekord University of Medical Sciences, Shahrekord, Iran.
Hearing loss (HL) is the most frequent sensory defect affecting 1 in 1000 neonates. This can occur due to genetic or environmental causes or both. The genetic causes are very heterogenous and over 100 loci have been identified to cause autosomal recessive non - syndromic hearing loss (ARNSHL). The aim of this study was to determine the contribution of the LRTOMT gene mutations in causing ARNSHL. One hundred fifty seven pupils affected with ARNSHL from Azarbaijan Sharghi, Kordestan, Gilan and Golestan provinces, north and west of Iran, were ascertained. In this descriptive - laboratory study, the presence of LRTOMT mutations were initially checked using PCR – Single - strand conformation polymorphism (SSCP) and heteroduplex analysis (HA) strategy. Samples with shifted bands on the gel were confirmed by DNA sequencing method. The PCR-SSCP/HA and the subsequent direct DNA sequencing showed no mutation in the population studied. We conclude that LRTOMT mutations have no role in causing sporadic deafness in the studied population. Further studies on other populations and samples could clarify the exact role of LRTOMT mutations.
Babol University of Medical Sciences
International Journal of Molecular and Cellular Medicine (IJMCM)
2251-9637
2
1
2013
3
1
Electrophoretic Pattern and Antibacterial Activity of Proteins from Vicia Faba Seed Extract
46
49
EN
Hassan
Hoda
Biological Control Department, National Institite of Plant Protection, Amol, Iran.
Fatemeh
Elmi
Department of Chemistry, University of Mazandaran, Babolsar, Iran.
Maryam Mytra
Elmi
Cellular and Molecular Biology Research Center, and Paramedical Department, Babol University of Medical Sciences, Babol, Iran.
ABSTRACT
Background
Antibiotic resistance makes antimicrobial peptides (AMPs) agents an alternative for treatment of pathogenic diseases. They are isolated from various animals invertebrates, vertebrates and plants. The present study shows the electrophoretic pattern of protein and peptides from Vicia Faba seed and reports our first attempt to study the antibacterial activity of Vicia faba seed extract.
Methods
100g grounded seed was extracted in extraction buffer(10 mM Na2HPO4, 15 mM NaH2PO4, 100mM KCl, 2 mM EDTA, 2mM Thiurea 1.5% PVPP). Following a 70% ammonium sulfate precipitation was carried out. The pellet was dialyses and resuspended in 50mM ammonium acetate, then used for gel filtration chromatography (Sephadex G75). The crude extract electrophoresis was carried out on 12% SDS- PAGE gel. Antibacterial activity on E.Coli and B. Subtilis from hospital infection was tested and evaluated by measuring the inhibition zone diameter observation.
Results
The SDS- PAGE gel electrophoresis shows the crude extract contains many proteins with different spectrum molecular weight of proteins and peptides. The inhibition zone doesn’t observe in antibacterial properties tests. So, our experiments don’t show any antibacterial activity on E.Coli and B. Subtilis from hospital infection.
Conclusion
In our study, bacteria from hospital infection resists to Vicia faba seed extract. Some other AMPs haven’t also shown any antimicrobial properties on clinical trail. To show the antibacterial activity of the crude Vicia faba seed extract, it should be tested on some standard bacteria.