@article{ author = {Jorgensen, Christian and Noël, Danièle}, title = {Mesenchymal stem cells in osteoarticular diseases: an update}, abstract ={Multipotent mesenchymal stromal cells or mesenchymal stem cells (MSCs) are mainly isolated from bone marrow or fat tissue. Because of their potential of multilineage differentiation towards bone, cartilage and fat tissue, they were initially evaluated to develop innovative strategies for tissue engineering applications. More recently, they have gained interest based on their immunomodulatory properties and have been tested in various clinical trials aiming at modulating the host immune response in graft-versus-host disease or autoimmune diseases. MSC-mediated immunomodulation occurs through the secretion of soluble mediators. The clinical applications of MSCs for rheumatic diseases are focusing on their potential to help tissue repair/regeneration and to prevent inflammation. The aim of the present review is to focus on the mechanisms by which MSCs might exhibit a therapeutic potential in rheumatology and present an update on the mechanisms involved in the therapeutic effect of MSCs. Special attention is given to their possible modulation for future innovative strategies.}, Keywords = {Mesenchymal stem cell, immunosuppression, arthritis, cartilage regeneration, cell therapy}, volume = {1}, Number = {1}, pages = {1-10}, publisher = {Babol University of Medical Sciences}, url = {http://ijmcmed.org/article-1-27-en.html}, eprint = {http://ijmcmed.org/article-1-27-en.pdf}, journal = {International Journal of Molecular and Cellular Medicine (IJMCM)}, issn = {2251-9637}, eissn = {2251-9645}, year = {2012} } @article{ author = {Kadu, Vishal and Ghaskadbi, Saroj S and Ghaskadbi, Surendr}, title = {Induction of secondary axis in hydra revisited: New insights into pattern formation}, abstract ={In 1909, several years before the famous `Organizer’ experiments of Spemann and Mangold, Ethel Browne demonstrated induction of a secondary axis in hydra by grafting a hypostome. Based on this and subsequent work, in the late sixties, Lewis Wolpert proposed the theory of morphogen gradients and positional information. We have studied secondary axis induction by hypostome and foot tissue using three species of hydra as well as transgenic, GFP-expressing lines of hydra. We have found that pieces of hypostome and complete foot of a donor hydra can induce a secondary axis all along (in upper, middle or lower parts of) the body column of a host hydra, both within and across species with comparable rates. Thus, contrary to the available literature, our results show that the host hypostome does not completely inhibit the induction of a secondary axis. The length of the induced axis though is determined by the position of the graft. By using GFP-expressing lines of hydra we have demonstrated that host ectodermal and endodermal cells actively contribute to the secondary axis. On comparison, the hypostome was found to be a stronger and dominant Organizer than the foot. Foot grafting experiments show a transient increase in the host length as well as the distance between the two Organizers. The length becomes normal once the grafted foot reaches the budding zone. Our work brings out several new aspects of the role of positional cues in pattern formation in hydra that can be now be explored at cellular and molecular levels.}, Keywords = {Hydra, interspecific grafting, morphogen gradients, organizer, induction of secondary axis}, volume = {1}, Number = {1}, pages = {11-20}, publisher = {Babol University of Medical Sciences}, url = {http://ijmcmed.org/article-1-25-en.html}, eprint = {http://ijmcmed.org/article-1-25-en.pdf}, journal = {International Journal of Molecular and Cellular Medicine (IJMCM)}, issn = {2251-9637}, eissn = {2251-9645}, year = {2012} } @article{ author = {Hosseini, Seyed Masoud and Amini, Elham and TavassotiKheiri, Masoumeh and Mehrbod, Parvaneh and Shahidi, Mahsa and Zabihi, Ebrahim}, title = {Anti-influenza Activity of a Novel Polyoxometalate Derivative (POM-4960)}, abstract ={There are many effective chemothereutic agents used in influenza disease which some of them inhibit virus replication by interfering with FluV (influenza virus) viral binding or its penetration into cell membrane. A series of polyoxometalates compounds such as POM-523 and PM-504 have been synthesized and have showed inhibitory effects on viruses. In this study we examined anti influenza activity of a novel polyoxometalate derivative (POM-4960) synthesized in the Faculty of Chemistry of Damghan University of Basic Sciences.To evaluate the anti-influenza activity of POM, following the treatment of FluV with POM at different temperatures and incubation periods, viral titer reduction was assessed by haemaglutination assay (HA). The 3-(4,5- dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was used to determine TCID50 (tissue culture infective dose) of virus, CC50 (median cytotoxic concentration) of POM, protection percentage and antiviral activity of POM in cell culture. RT-PCR and direct Immunofluorescent assays were performed to evaluate the effect of POM on viral infection and viral RNA load, respectively. POM reduced HA titer near to zero in all cell culture specimens and showed high protection against viral infection of the cells. Reduction in viral infection was confirmed by RT-PCR and Immunofluorescent staining methods. Moreover, this POM derivative has a dual (cumulative) effect on attachment and penetration inhibition compared to other POM’s with just one inhibitory effect. POM-4960 could be considered as a powerful anti-influenza agent with low toxicity and high antiviral potency.}, Keywords = {POM, influenza virus, RT-PCR, immunofluorescent, MTT}, volume = {1}, Number = {1}, pages = {21-29}, publisher = {Babol University of Medical Sciences}, url = {http://ijmcmed.org/article-1-33-en.html}, eprint = {http://ijmcmed.org/article-1-33-en.pdf}, journal = {International Journal of Molecular and Cellular Medicine (IJMCM)}, issn = {2251-9637}, eissn = {2251-9645}, year = {2012} } @article{ author = {Carata, Elisabetta and AnnaTenuzzo, Bernardetta and Federica, Arnò and Buccolieri, Alessandro and Serra, Antonio and Manno, Daniela and Dini, Luci}, title = {Stress response induced by carbon nanoparticles in Paracentrotus lividus}, abstract ={Members of the 14-3-3 protein family are involved in many important cellular events, including stress response, survival and apoptosis. Genes of the 14-3-3 family are conserved from plants to humans, and some members are responsive to UV radiation. Despite the high rate of pollution generated by nano-pollutants, up to now is totally obscure their toxic effect on development. Embryos treated with carbon nanoparticles, RNA preparation, retro-transcription and quantitative real-time PCR. In response to carbon nano-particles exposure, the embryos collected 24 h later showed a 3,07-fold at 5x1012 p and a 1,58-fold at 2.5x1013 p and a 1,92-fold at 2.5x1014 p increase in Pl14-3-3ε transcript levels compared with controls. The Pl14-3-3ε mRNA delocalization parallels the failure in archenteron elongation observed morphologically, as well as the lack of specific endoderm markers. Here, we report the isolation of the complete cDNA encoding the 14-3-3 epsilon isoform from Paracentrotus lividus sea urchin embryos, referred to as Pl14-3-3ε. Pl14-3-3ε mRNA levels were measured by RT-PCR during development and found to increase from the mesenchyme blastula to the prism stage. Our results confirm the involvement of 14-3-3ε in the stress response elicited by carbon nano-particles.}, Keywords = {Paracentrotus lividus, carbon nanoparticles, toxicity, 14-3-3 epsilon, gene expression}, volume = {1}, Number = {1}, pages = {30-38}, publisher = {Babol University of Medical Sciences}, url = {http://ijmcmed.org/article-1-26-en.html}, eprint = {http://ijmcmed.org/article-1-26-en.pdf}, journal = {International Journal of Molecular and Cellular Medicine (IJMCM)}, issn = {2251-9637}, eissn = {2251-9645}, year = {2012} } @article{ author = {Hassanzadeh-Nazarabadi, Mohammad and Sanjarmoosavi, Nasrin and Sanjarmoosavi, Naser}, title = {Cleft Palate induced by Sulfur Mustard in mice fetus}, abstract ={Sulfur Mustard (SM) is a chemical warfare agent which was widely used in the World War I and more recently during Gulf war in the early 1980s'. SM is a strong alkylating agent with known mutagenic and carcinogenic effects but only few studies have been published on its teratogenicity. Since SM has been widely used as a chemical weapon by the Iraqi regime against the Iranian soldiers as well as the civilian population particularly pregnant women in the border area therefore, the investigation of SM adverse effects on cleft malformations which is one of the most frequent congenital anomalies is considered in this study. An experimental work has been carried out in embryopathy in mouse with intraperitoneal injection of 0.75 and 1.5 mg/kg SM at different periods of gestation. Cleft lip and palate were examined by stereomicroscopy. Current data demonstrate that exposure with SM on the 11th day of gestation can increase the incidence of cleft defects in comparison with control group (P<0.001). These results also show that SM treatment in GD 11 and 13 can lead to more anomalies compared with GD 14 (P<0.001). They also show that the teratogenic effects of SM are restrictively under the influence of the threshold dose and time of gestation. The present results suggest that exposure to sufficient doses of SM on critical days of gestation may increase the risk of congenital cleft malformations.}, Keywords = {Sulfur Mustard, teratogenicity, cleft lip/palate}, volume = {1}, Number = {1}, pages = {39-43}, publisher = {Babol University of Medical Sciences}, url = {http://ijmcmed.org/article-1-36-en.html}, eprint = {http://ijmcmed.org/article-1-36-en.pdf}, journal = {International Journal of Molecular and Cellular Medicine (IJMCM)}, issn = {2251-9637}, eissn = {2251-9645}, year = {2012} } @article{ author = {Tatar, Mohsen and Qujeq, Durdi and Feizi, Farideh and Parsian, Hadi and SohanFaraji, Alieh and Halalkhor, Sohrab and Abassi, Roya and Abedian, Zeinab and Pourbagher, Roughayeh and AghajanpourMir, Seyed Mohsen and Mir, Hamed and Seyfizadeh, Nayer}, title = {Effects of Teucrium Polium Aerial Parts extract on oral glucose tolerance tests and pancreas histopathology in Streptozocin-induced diabetic rats}, abstract ={Teucrium polium can reduce serum glucose. There are few reports in the literature related to this subject and the resolution of this mechanism requires further experiments. The aim of the present study was to evaluate the effects of Teucrium polium aerial parts extracts on oral glucose tolerance tests and pancreas histology in streptozocin-induced diabetic rats. In order to prepare the aqueous concentrate, aerial parts extract was dissolved in distilled water and was boiled for 30 minutes. For the preparation of ethanolic solution, powder was dissolved in ethanol and mixed by a shaker. Diabetic rats were induced with single IP injection of streptozotocin (STZ) at a dose of 50 mg/kg body weight dissolved in normal saline just before use to the 16 hr fast rats. Both groups, diabetic and normal were sacrificed by ether anesthesia. The tissue samples were formalin fixed and paraffin embedded for microscopic examination in accordance with routine laboratory procedures. Blood was collected from the tail vein of the rats. Serum glucose levels were then measured by commercial kits by using a glucose oxidized method. There were no biochemical abnormalities or histological changes in the pancreas of control rats. Post treatment of Teucrium polium aerial parts extract reduced the severity of streptozotocin diabetic pancreases. Our histopathological investigation along with the biochemical evaluations showed a significant effect on histological changes in the pancreas of induced diabetic rats upon Teucrium polium aerial parts extract treatment (P<0.05).}, Keywords = {Teucrium polium, oral glucose tolerance tests, pancreas histology, streptozocin, diabetic rats}, volume = {1}, Number = {1}, pages = {44-49}, publisher = {Babol University of Medical Sciences}, url = {http://ijmcmed.org/article-1-37-en.html}, eprint = {http://ijmcmed.org/article-1-37-en.pdf}, journal = {International Journal of Molecular and Cellular Medicine (IJMCM)}, issn = {2251-9637}, eissn = {2251-9645}, year = {2012} } @article{ author = {Ghaderian, Sayyed Mohammad Hossein and Khodaii, Zohreh}, title = {Tissue remodeling investigation in varicose veins}, abstract ={Although the etiology of varicose veins remains unknown, recent studies have focused on endothelial cell integrity and function because the endothelium regulates vessel tone and synthesizes many pro- and anti-inflammatory factors. The aim of this study was to investigate the evidence involving the endothelium in the development of varicose vein disease. In addition, tissue remodeling was investigated in varicose veins to determine the expression of different types of collagen. Tissue specimens of superficial varicose veins and control saphenous vein were used for immunohistochemical and transmission electron microscope (TEM). α-smooth muscle actin, and collagen I, III, IV antibodies were applied for immunohistochemical investigation. Findings of this study showed alterations of the intima, such as focal intimal discontinuity and denudation of endothelium and the media, such as irregular arrangements of smooth muscle cells and collagen fibres in varicose veins. Our findings showed some changes in terms of distribution of types I, III and IV collagen in the intima and media of varicose vein walls compared with controls. These alterations to the media suggest that the pathological abnormality in varicose veins may be due to the loss of muscle tone as a result of the breakup of its regular structure by the collagen fibres. These findings only described some changes in terms of distribution of these types of collagen in the intima and media of varicose vein walls which may result in venous wall dysfunction in varicosis.}, Keywords = {Varicose vein disease, endothelial cells, transmission electron microscopy, collagen fibres}, volume = {1}, Number = {1}, pages = {50-61}, publisher = {Babol University of Medical Sciences}, url = {http://ijmcmed.org/article-1-30-en.html}, eprint = {http://ijmcmed.org/article-1-30-en.pdf}, journal = {International Journal of Molecular and Cellular Medicine (IJMCM)}, issn = {2251-9637}, eissn = {2251-9645}, year = {2012} } @article{ author = {Pervez, Shahi}, title = {Non-Hodgkin Lymphoma (NHL) in Pakistan}, abstract ={Lymphomas are classified as Hodgkin’s and Non-Hodgkin’s lymphomas (HL NHL) NHL being further sub-divided into B, T and Null cell categories on the basis of WHO classification. With a few exceptions worldwide, B-NHL are more common, accounting approximately 80-85% of all cases of NHL compared to T-NHL, which accounts for about 10-15% of all NHL cases. The incidence of NHL has shown a steady increase and attention is being focused on the possible causes of this increase. Epidemiologic studies indicate that environmental factors do play a role in the causation of NHL, such as drugs, pesticides, solvents, hair dyes, viruses (EBV, HTLV-1, Hepatitic C and HIV) and Helicobacter pylori. Hence many different environmental factors of risk acting on large segments of the population can contribute for increase of NHL.}, Keywords = {Non-Hodgkin, lymphoma, NHL, Pakistan}, volume = {1}, Number = {1}, pages = {62-63}, publisher = {Babol University of Medical Sciences}, url = {http://ijmcmed.org/article-1-29-en.html}, eprint = {http://ijmcmed.org/article-1-29-en.pdf}, journal = {International Journal of Molecular and Cellular Medicine (IJMCM)}, issn = {2251-9637}, eissn = {2251-9645}, year = {2012} } @article{ author = {Caruso, Maddalena and Evangelista, Marco and Parolini, Ornell}, title = {Human term placental cells: phenotype, properties and new avenues in regenerative medicine}, abstract ={The human placenta has long been the subject of scientific interest due to the important roles which it performs during pregnancy in sustaining the fetus and maintaining fetomaternal tolerance. More recently, however, researchers have begun to investigate the possibility that the placenta’s utility may extend beyond fetal development to act as a source of cells with clinically relevant properties. Indeed, several groups have reported the isolation of cells from different placental regions which display both multilineage differentiation potential and immunomodulatory properties in vitro. Furthermore, these cells have also been shown to secrete soluble factors involved in pathophysiological processes that may aid tissue repair. Cells with such features will clearly find application in the field of regenerative medicine for the repair/regeneration of damaged or diseased tissues or organs. In line with these promising findings, several preclinical and clinical studies conducted to date argue in strong favor of the therapeutic utility of placenta-derived cells for the treatment of several diseases. Although much work remains to be conducted in order to fully understand the properties of placental cells and the mechanisms which underlie their beneficial effects in vivo, data reported to date nonetheless provide compelling evidence in support of the placenta as a cell source for use in regenerative medicine.}, Keywords = {Amnion, chorion, placenta, regenerative medicine, stem cells}, volume = {1}, Number = {2}, pages = {64-74}, publisher = {Babol University of Medical Sciences}, url = {http://ijmcmed.org/article-1-39-en.html}, eprint = {http://ijmcmed.org/article-1-39-en.pdf}, journal = {International Journal of Molecular and Cellular Medicine (IJMCM)}, issn = {2251-9637}, eissn = {2251-9645}, year = {2012} } @article{ author = {Mulherkar, Rita and Husain, Tabish}, title = {Lymphoblastoid cell lines: a continuous in vitro source of cells to study carcinogen sensitivity and DNA repair}, abstract ={Obtaining a continuous source of normal cells or DNA from a single individual has always been a rate limiting step in biomedical research. Availability of Lymphoblastoid cell lines (LCLs) as a surrogate for isolated or cryopreserved peripheral blood lymphocytes has substantially accelerated the process of biological investigations. LCLs can be established by in vitro infection of resting B cells from peripheral blood with Epstein Barr Virus (EBV) resulting in a continuous source, bearing negligible genetic and phenotypic alterations. Being a spontaneous replicating source, LCLs fulfil the requirement of constant supply of starting material for variety of assays, sparing the need of re-sampling. There is a reason to believe that LCLs are in close resemblance with the parent lymphocytes based on the ample supporting observations from a variety of studies showing significant level of correlation at molecular and functional level. LCLs, which carry the complete set of germ line genetic material, have been instrumental in general as a source of biomolecules and a system to carry out various immunological and epidemiological studies. Furthermore, in recent times their utility for analysing the whole human genome has extensively been documented. This proves the usefulness of LCLs in various genetic and functional studies. There are a few contradictory reports that have questioned the employment of LCLs as parent surrogate. Regardless of some inherent limitations LCLs are increasingly being considered as an important resource for genetic and functional research.}, Keywords = {Lymphoblastoid cell lines, Epstein Barr virus, cell immortalization, carcinogen sensitivity, DNA damage/repair }, volume = {1}, Number = {2}, pages = {75-87}, publisher = {Babol University of Medical Sciences}, url = {http://ijmcmed.org/article-1-28-en.html}, eprint = {http://ijmcmed.org/article-1-28-en.pdf}, journal = {International Journal of Molecular and Cellular Medicine (IJMCM)}, issn = {2251-9637}, eissn = {2251-9645}, year = {2012} } @article{ author = {Nazarpour, Robab and Zabihi, Ebrahim and Alijanpour, Ebrahim and Abedian, Zeinab and Mehdizadeh, Hamid and Rahimi, Fatemeh}, title = {Optimization of human peripheral blood mononuclear cells (PBMCs) cryopreservation}, abstract ={Cryopreservation is the method of choice for long term storage of human PBMCs. This study was designed to compare the different combinations of variables affecting the cryopreservation of PBMCs samples. The viability of PBMCs separated from 2×5 ml peripheral blood samples obtained from 16 healthy adult volunteers, were measured using trypan blue dye exclusion method just before freezing with different concentrations of DMSO (10, 15, and 20%) and FBS (40 or 70%) at two different temperatures (either 4oC or 25oC). Then after 2 weeks the cells were thawed and the viability was measured again. Also the PBMCs response to PHA was measured after 48 h using MTT assay. The effects of the different variables were calculated and compared among the groups. A total of 192 PBMCs cryotubes made from blood samples of 16 volunteers were tested. The viability of the cells obtained by the two centrifugation procedure was the same (both more than 99%). The concentration of the FBS (40 vs 70%) did not show to have significant effects on either cells viability or response to PHA. On the other hand 20% DMSO concentration and freezing temperature at 25oC decreased both cells. Based on the obtained results, it is recommended to centrifuge the PBMCs under higher revolt speed at shorter time (700 g for 20 minutes) and decrease the FBS concentration to 40%. The DMSO concentration should be kept at 10-15% and the freezing medium be cooled down to 4oC.}, Keywords = {PBMCs, ficol, freezing, DMSO, FBS}, volume = {1}, Number = {2}, pages = {88-93}, publisher = {Babol University of Medical Sciences}, url = {http://ijmcmed.org/article-1-48-en.html}, eprint = {http://ijmcmed.org/article-1-48-en.pdf}, journal = {International Journal of Molecular and Cellular Medicine (IJMCM)}, issn = {2251-9637}, eissn = {2251-9645}, year = {2012} } @article{ author = {Shokravi, Marziyeh and Tabarsi, Bahareh and Moghaddamnia, Aliakbar and Sohanfaraji, Alieh and Pourghasem, Mohse}, title = {Comparison of skin reaction between MTA (produced in Iran) and CEM in Rabbit}, abstract ={Pathological changes in pulp and periapical tissues are addressed by endodontic treatment. The material used in this treatment must be biocompatible. The aim of this study is to compare the skin reaction of Calcium Enriched Mixture (CEM) and Mineral Trioxide Aggregate (MTA) produced in Iran on rabbit. Sixteen male newzeland albino rabbits weighting 2 kg were used. The animals back hair was shaved, 24 hours before application of each material. The material was applied on two sites (2 × 2 cm) while the third site was used as control. All sites were covered by gauze and bandaged for 4 hours. Then the material's remnants were washed off the sites of application. Observations were performed in 1, 24, 48 and 72 hours after removing the materials. Erythematous surface areas were measured by the morphometric method. After sacrificing animals the skins were dissected and the specimens were prepared for histological evaluation. There were significant differences between CEM and MTA in erythematous surface areas at 1, 24 and 48 hours after removing the materials (p0.05). The average erythematous surface areas were wider in MTA sites than CEM sites. As a conclusion it seems that biocompatibility of CEM could be more than MTA.}, Keywords = {Skin reaction , MTA made in Iran, CEM}, volume = {1}, Number = {2}, pages = {94-98}, publisher = {Babol University of Medical Sciences}, url = {http://ijmcmed.org/article-1-44-en.html}, eprint = {http://ijmcmed.org/article-1-44-en.pdf}, journal = {International Journal of Molecular and Cellular Medicine (IJMCM)}, issn = {2251-9637}, eissn = {2251-9645}, year = {2012} } @article{ author = {Hassanzadeh-Nazarabadi, Mohammad and Shekouhi, Sahar and Seif, Najmeh}, title = {The incidence of spontaneous abortion in mothers with blood group O compared with other blood types}, abstract ={Although ABO incompatibility between mother and fetus has long been suspected as cause of spontaneous abortion in man, its precise contribution has not been completely resolved. In spite of reports in which the incompatible mating was recognized to be a cause of habitual abortion, and which eventually results in infertility or a reduction in the number of living children compared with the number in compatible matings, such effects were not observed in other studies. The aim of this review article was to show some evidence of relationship between ABO incompatibility and spontaneous abortion.}, Keywords = {spontaneous abortion, ABO blood group, incompatibility }, volume = {1}, Number = {2}, pages = {99-104}, publisher = {Babol University of Medical Sciences}, url = {http://ijmcmed.org/article-1-40-en.html}, eprint = {http://ijmcmed.org/article-1-40-en.pdf}, journal = {International Journal of Molecular and Cellular Medicine (IJMCM)}, issn = {2251-9637}, eissn = {2251-9645}, year = {2012} } @article{ author = {Dasar, Negin and Ghaderian, Sayyed Mohammad Hossein and Azargashb, Eznollah}, title = {Human Evaluation of the Glu298Asp polymorphism in NOS3 gene and its relationship with onset age of ESRD in Iranian patients suffering from ADPKD}, abstract ={One of the most striking features in autosomal dominant polycystic kidney disease (ADPKD) is the difference at onset age of end-stage renal disease (ESRD). Modifier genes may play a role in this phenotypic variability. The mutated nitric oxide synthase 3 gene (NOS3), have a modifier effect on the severity of ADPKD by impairment of NOS3 activity and decreasing of renal vascular nitric oxide production and, subsequently, reduced kidney function. In order to test this hypothesis, we investigated the relationship between Glu298Asp polymorphism in exon 7 of this gene and ESRD in ADPKD patients refered from Shahid Labbafi Nedjad Hospital in Tehran. The polymorphism was examined by PCR, followed by RFLP (with MboI) in three groups of ADPKD with ESRD ADPKD without ESRD patients and normal individual as the cases, case-controls and controls, respectively. The frequencies of GG, GT, and TT genotypes in cases were 66.7%, 33.3% and 0%, in case-controls were 78.6%, 19%, 2.4%, and in controls were 64.3%, 35.7% and 0%, respectively. Our findings revealed that there was no significant difference in the genotype frequency of NOS3 gene in ADPKD patients (p=0.311).The age of onset of ESRD in ADPKD patients, harbouring the T allele of this polymorphism, was two years lower than G/G patients, but this difference was not significant (p =0.641). In conclusion, our results suggest that there is no evidence of relationship between Glu298Asp polymorphism and onset age of ESRD in Iranian ADPKD patients.}, Keywords = {ADPKD, ESRD, NOS3, Glu298Asp polymorphism}, volume = {1}, Number = {2}, pages = {105-112}, publisher = {Babol University of Medical Sciences}, url = {http://ijmcmed.org/article-1-49-en.html}, eprint = {http://ijmcmed.org/article-1-49-en.pdf}, journal = {International Journal of Molecular and Cellular Medicine (IJMCM)}, issn = {2251-9637}, eissn = {2251-9645}, year = {2012} } @article{ author = {KholghiOskooei, Vahid and EsmaeiliDooki, Mohammad Reza and Akhavan-Niaki, Haleh}, title = {Analysis of c.3369+213TA[7-56] and D7S523 microsatellites linked to Cystic Fibrosis Transmembrane Regulator.}, abstract ={  Cystic fibrosis (CF) is a life-limiting autosomal recessive disorder affecting principally respiratory and digestive system . It is caused by cystic fibrosis transmembrane conductance regulator (CFTR) gene mutation. The aim of this study was to determine the extent of repeat numbers and the degree of heterozygosity for c.3499+200TA(7_56) and D7S523 located in intron 17b and 1 cM proximal to the CFTR gene respectively. Both microsatellites were analyzed by direct electrophoresis of PCR product on 20% polyacrylamide gel in 40 Normal subjects and 40 CF patients originating from North Iran. 9 different alleles were found for D7S523 ranging from 16 to 24 repeats alleles. (CA)20 was the most prevalent allele both in normal individuals and CF patients with 21.3% and 20% frequencies respectively. Heterozygosity frequency of D7S523 in normal individuals and CF patients was 97.5% and 90% respectively. Eighteen different alleles were found for c.3499+200TA(7_56) ranging from 8 to 38 repeats alleles. (TA)9 was the most prevalent allele both in normal individuals and CF patients with 30% and 23.5% frequencies respectively. All normal subjects and 97.5% of CF patients showed heterozyous genotype. The high heterozygosity of the two studied microsatellites witnesses the dynamism of such markers. High degree of heterozygosity of c.3499+200TA(7_56) and D7S523 make these markers, a very useful tool for prenatal diagnosis especially in Iranian population.}, Keywords = {Cystic Fibrosis, c.3369+213TA[7-56], D7S523, Iran}, volume = {1}, Number = {2}, pages = {113-118}, publisher = {Babol University of Medical Sciences}, url = {http://ijmcmed.org/article-1-51-en.html}, eprint = {http://ijmcmed.org/article-1-51-en.pdf}, journal = {International Journal of Molecular and Cellular Medicine (IJMCM)}, issn = {2251-9637}, eissn = {2251-9645}, year = {2012} } @article{ author = {Hollweck, Trixi and Hagl, Christian and Eissner, Günther}, title = {Mesenchymal stem cells from umbilical cord tissue as potential therapeutics for cardiomyodegenerative diseases – a review}, abstract ={Heart failure is one of the leading causes of death worldwide. End stage disease often requires heart transplantation, which is hampered by donor organ shortage. Tissue engineering represents a promising alternative approach for cardiac repair. For the generation of artificial heart muscle tissue several cell types, scaffold materials and bioreactor designs are under investigation. In this review, the use of mesenchymal stem cells derived from human umbilical cord tissue (UCMSC) for cardiac tissue engineering will be discussed.}, Keywords = {Umbilical cord tissue, mesenchymal stem cells, cardiac differentiation, non-degradable scaffolds, polytetrafluorethylene, titanium, bioreactor}, volume = {1}, Number = {3}, pages = {119-132}, publisher = {Babol University of Medical Sciences}, url = {http://ijmcmed.org/article-1-45-en.html}, eprint = {http://ijmcmed.org/article-1-45-en.pdf}, journal = {International Journal of Molecular and Cellular Medicine (IJMCM)}, issn = {2251-9637}, eissn = {2251-9645}, year = {2012} } @article{ author = {M.Samarasinghe, Rasika and K.Kanwar, Rupinder and R.Kanwar, Jagat}, title = {The Role of Nanomedicine in Cell Based Therapeutics in Cancer and Inflammation}, abstract ={Cell based therapeutics is one of the most rapidly advancing medical fields, bringing together a range of fields including transplantation, tissue engineering and regeneration, biomaterials and stem cell biology. However, traditional cell-based therapeutics have many limitations, one of which is their harmful effects exhibited on healthy body cells due to their lack of specificity. Nanomedicine is providing an alternative treatment strategy that is more targeted and specific to a range of diseases. Varying from polymers conjugated with drugs or tissue targeting molecules, to proteins encapsulated within a polymer shell, nanomedicine will without a doubt play a major role in designing effective cell-based therapeutics that can overcome certain classical problems. These may include from addressing the problem of non-specificity of contemporary treatments to overcoming mechanical barriers, such as crossing cell membranes. This review summarises the recent work on nano-based cell therapy as a regenerative agent and as a therapeutic for cancer and neurological diseases.}, Keywords = {Nanomedicine, cell therapy, cancer, blood brain barrier , inflammation}, volume = {1}, Number = {3}, pages = {133-144}, publisher = {Babol University of Medical Sciences}, url = {http://ijmcmed.org/article-1-38-en.html}, eprint = {http://ijmcmed.org/article-1-38-en.pdf}, journal = {International Journal of Molecular and Cellular Medicine (IJMCM)}, issn = {2251-9637}, eissn = {2251-9645}, year = {2012} } @article{ author = {Ataie, Amin and Ataee, Ramin and Shadifar, Mohmmad and Shahabi, Sima and AghajanpourMir, Seyed Mohsen and Hosseinpour, Younes}, title = {Interaction of Memantine with Homocysteine on the Apoptosis in the Rat Hippocampus cells}, abstract ={It has been hypothesized that elevated plasma Homocysteine (Hcy) plays a role in the pathogenesis of Alzheimer’s disease (AD) and age-related cognitive decline. The mechanism of Hcy neurotoxicity in the brain is controversial as well Hcy is a ligand of NMDA receptor. Memantine, an uncompetitive antagonist of N-methyl-D-aspartate (NMDA) receptors approved for the treatment of moderate to severe Alzheimer's disease. Hcy was injected 0.5 μmol/μl in the hippocampus of the rat brain and Memantine hydrochloride was injected 10mg/kg intraperitoneally 1 hour prior to Hcy injection. After five days, rats were killed and whole brain were taken out, fixed, and embedded in paraffin. The slices of the rat brain were prepared and immunohistochemical analysis was done to reveal the protein expression of Bax, Bcl-2, and the activation of Caspase 3 in the rat hippocampus layers. Results showed significant increase of Bax and Caspase-3 immunoreactivity in hippocampus of rat brain in Hcy group. Also an increase in Bax/Bcl-2 ratio in rat hippocampus cells .Memantine pretreatment could not change the levels of Bax, Bcl-2, Caspase-3 significantly in rat’s hippocampus cells. These findings suggest that Memantine could not antagonize Hcy – induced Apoptosis. Hcy may induce apoptosis via the other oxidative stress mechanism in the rat brain. potential. It may therefore be interesting that he barberry fruit extracts has the unique capacity to quench free radicals.}, Keywords = {Homocysteine, apoptosis. memantine, alzheimer’s disease, hippocampus, NMDA receptor, oxidative stress}, volume = {1}, Number = {3}, pages = {145-152}, publisher = {Babol University of Medical Sciences}, url = {http://ijmcmed.org/article-1-52-en.html}, eprint = {http://ijmcmed.org/article-1-52-en.pdf}, journal = {International Journal of Molecular and Cellular Medicine (IJMCM)}, issn = {2251-9637}, eissn = {2251-9645}, year = {2012} } @article{ author = {Ghaffari, Salman and Kalantari, Narges}, title = {Molecular analysis of 18S rRNA gene of Cryptosporidium parasites from patients living in Iran, Malawi, Nigeria and Vietnam}, abstract ={  Cryptosporidium species are one of the most common causes of gastrointestinal infection in humans around the world. This study has aimed to investigate the hyper variable region of the 18S rRNA gene in Cryptosporidium for exact parasite identification .   DNA was extracted from 26 fecal samples from which initially Cryptosporidium oocysts were identified by Ziehl-Neelsen acid-fast , Auramine phenol and ELISA techniques. Nested PCR, targeting the most polymorphic region of the 18S rRNA gene and genotyping was performed by restriction endonuclease digestion of the PCR product followed by nucleotide sequencing and phylogenic analysis.   Among 26 isolates analyzed, three species of Cryptosporidium were identified 38.5% of the isolates were C. hominis while 53.8% of the isolates were C. parvum and 7.7% of the isolates were C. meleagridis, which the last two species have the potentially zoonotic transmission. The only 11T subtype of C. hominis was demonstrated. These strains clustered distinctly into either human or animal origin regardless of the geographical origin, age, or immunity status of the patients. In summary, this work is the first report of C. meleagridis infecting human in Iran. Moreover, it suggested that multi-locus study of Cryptosporidium species in developing countries would be necessary to determine the extent of transmission of cryptosporidiosis in the populations.}, Keywords = {18S rRNA, Cryptosporidium, diarrhea, developing countries }, volume = {1}, Number = {3}, pages = {153-161}, publisher = {Babol University of Medical Sciences}, url = {http://ijmcmed.org/article-1-57-en.html}, eprint = {http://ijmcmed.org/article-1-57-en.pdf}, journal = {International Journal of Molecular and Cellular Medicine (IJMCM)}, issn = {2251-9637}, eissn = {2251-9645}, year = {2012} } @article{ author = {Akhavan-Niaki, Haleh and YoussefiKamangari, Reza and Banihashemi, Ali and KholghiOskooei, Vahid and Azizi, Mandana and Tamaddoni, Ahmad and Sedaghat, Sadegh and Vakili, Mohsen and MahmoudiNesheli, Hassan and Shabani, Soray}, title = {Hematologic Features of Alpha Thalassemia Carriers}, abstract ={Alpha thalassemia (α-thal) is relatively common worldwide. Most carriers are defective in either one or two alpha globin genes out of four functional ones, with deletions being more common than point mutations. The hematologic features are very important for the selection of the appropriate molecular tests while determining the genotype. The aim of this study was to compare hematologic features of patients with various types of α globin mutations. Hematological indices including red blood cells (RBC), hemoglobin concentration (Hb), mean cell volume (MCV), mean cell hemoglobin (MCH), Mean corpuscular hemoglobin concentration (MCHC) and percentage of Hemoglobin (HBA1, HBA2 and HBF) of seven-hundred and twenty two patients presenting ten different α-thal genotypes were considered. All patients showed reduced MCV and/or MCH values. Moreover, MCV and MCH were lower in patients with two functional alpha globin genes in comparison to patients with one mutated alpha globin gene (P value<0.001). In conclusion, MCV and MCH values can be helpful for the selection of the appropriate molecular tests to determine the genotype of alpha thalassemia carriers.}, Keywords = {Alpha thalassemia, Mean cell volume, Mean cell hemoglobin, Mutation }, volume = {1}, Number = {3}, pages = {162-167}, publisher = {Babol University of Medical Sciences}, url = {http://ijmcmed.org/article-1-62-en.html}, eprint = {http://ijmcmed.org/article-1-62-en.pdf}, journal = {International Journal of Molecular and Cellular Medicine (IJMCM)}, issn = {2251-9637}, eissn = {2251-9645}, year = {2012} } @article{ author = {Qujeq, Durdi and Kamei, Solmaz}, title = {In vitro antioxidant effects of barberry fruit extracts}, abstract ={A vast majority of the studies addressing the free radicals including hydroxyl radical is a damage compound of biochemical molecules such as DNA, proteins and lipids. When free radicals specially hydroxyl radical are not adequately removed from the body, it may damage biological macromolecules, leading to a variety of disease occurs. Therefore, the body should be protected by an enzymatic or non-enzymatic antioxidant defense system against free radicals. In order to explore the hypothesis that antioxidant plants can serve as therapeutic agents for diseases, the effect of Barberry fruit extracts was studied in an in vitro model. By evaluating their scavenging potential. Barberry fruits were collected from Babol, Iran and certified by the local scientist Mazandaran Province, Iran. The Barberry fruits were cleaned and dried at room temperature while keeping away from direct sunlight and then powdered. Suitable amounts of dried plant were coarsely grounded and used for extraction. The dry plant samples were extracted with water and/or ethanol. 10 g of Barberry fruits extracts powder was percolated by water for 24 hours. The extract was filtered and concentrated. Hydroxyl radical was produced as described previously. Then, Barberry fruits hydroxyl radical scavenging capacity was determined using deoxyribose degradation system, followed spectrophotometrically at 532 nm. As expected ,our data indicate that the level of hydroxyl radical generation in with aqueous and /or ethanol extracts of barberry fruit was decreased in comparison without barberry fruit extract in vitro system [(6.11±0.83, 5.28 ±1.44, mmol/ml) vs. (9.32±0.38, mmol/ml)], p<0.05, respectively. Indeed, our results revealed that the extracts of the Barberry fruit scavenge hydroxyl radical in vitro sample as compared to the controls. The barberry fruit extracts proved to be an effective for hydroxyl radical scavenging. The present data revealed that beneficial effect of Barberry fruit aqueous and ethanol extracts may be due to its free radical scavenging potential. It may therefore be interesting that he barberry fruit extracts has the unique capacity to quench free radicals.}, Keywords = {Antioxidant, barberry fruit, aqueous and ethanol extracts, hydroxyl radical}, volume = {1}, Number = {3}, pages = {168-172}, publisher = {Babol University of Medical Sciences}, url = {http://ijmcmed.org/article-1-46-en.html}, eprint = {http://ijmcmed.org/article-1-46-en.pdf}, journal = {International Journal of Molecular and Cellular Medicine (IJMCM)}, issn = {2251-9637}, eissn = {2251-9645}, year = {2012} } @article{ author = {Morovvati, Saeid and AmirpourAmaraii, sara and Zahedshekarabi, hosna and Shahbazi, nastar}, title = {Osteopetrosis a report of two Iranian patients with autosomal recessive inheritance pattern}, abstract ={In the rare hereditary bone disorder of osteopetrosis, reduced bone resorption function leads to both the development of densely sclerotic fragile bones and progressive obliteration of the marrow spaces and cranial foramina. Marrow obliteration, typically associated with extramedullary hemopoiesis and hepatosplenomegaly, results in anemia and thrombocytopenia and nerve entrapment accounts for progressive blindness and hearing loss. Severe infantile or malignant osteopetrosis is the worst type of the disease which has poor prognosis. In this study we report two cases of severe infantile or malignant type of the disease in an Iranian family. Our two patients were children of a family where the wife is a grandchild of the husband’s aunt. The first patient had episodes of seizure and spastic in extremities 2 weeks after birth. Gradually, the patient showed upper and lower respiratory problems and horizontal nystagmus. X-Ray of hand and foot showed widening and increased bone density and physical examination showed hepatosplenomegallay and petechiae in extremities. The patient expired due to cardiopulmonary arrest. The second patient had also episodes of seizure 2 weeks after birth. Gradually, dissymmetry in eyes appeared and blindness was confirmed by ophthalmologist. Finally the patient expired because of severe pneumonia. Autosomal recessive osteopetrosis has been reported in most ethnic groups although it is more frequently seen in ethnic groups where consanguinity is common. We report for the first time two cases of severe infantile or malignant type of the disease in an Iranian family.}, Keywords = {Osteopetrosis, Autosomal recessive, Consanguinity}, volume = {1}, Number = {3}, pages = {173-177}, publisher = {Babol University of Medical Sciences}, url = {http://ijmcmed.org/article-1-50-en.html}, eprint = {http://ijmcmed.org/article-1-50-en.pdf}, journal = {International Journal of Molecular and Cellular Medicine (IJMCM)}, issn = {2251-9637}, eissn = {2251-9645}, year = {2012} } @article{ author = {Halimi, Mohammad and Asghari, Mohsen and Sariri, Reyhaneh and Moslemi, Dariush and Parsian, Hadi}, title = {Cellular response to ionizing radiation: A microRNA story}, abstract ={MicroRNAs (miRNAs) represent a class of small non-coding RNA molecules that regulate gene expression at the post-transcriptional level. They play a crucial role in diverse cellular pathways. Ionizing radiation (IR) is one of the most important treatment protocols for patients that suffer from cancer and affects directly or indirectly cellular integration. Recently it has been discovered that microRNA-mediated gene regulation interferes with radio-related pathways in ionizing radiation. Here, we review the recent discoveries about miRNAs in cellular response to IR. Thoroughly understanding the mechanism of miRNAs in radiation response, it will be possible to design new strategies for improving radiotherapy efficiency and ultimately cancer treatment.}, Keywords = {Cellular response, ionizing radiation, microRNA}, volume = {1}, Number = {4}, pages = {178-184}, publisher = {Babol University of Medical Sciences}, url = {http://ijmcmed.org/article-1-67-en.html}, eprint = {http://ijmcmed.org/article-1-67-en.pdf}, journal = {International Journal of Molecular and Cellular Medicine (IJMCM)}, issn = {2251-9637}, eissn = {2251-9645}, year = {2012} } @article{ author = {Bhuin, Tanmay and KumarRoy, Jagat}, title = {Rab11 is required for maintenance of cell shape via βPS integrin mediated cell adhesion in Drosophila}, abstract ={In eukaryotes, vesicle trafficking is regulated by the small monomeric GTPases of the Rab protein family. Rab11, (a subfamily of the Ypt/Rab gene family) an evolutionarily conserved, ubiquitously expressed subfamily of small monomeric Rab GTPases, has been implicated in regulating vesicular trafficking through the recycling of endosomal compartment. In an earlier communication, we have shown that Rab11 is required for cell adhesion, maintenance of cell shape and actin-cytoskeleton organization during Drosophila wing development. Here, we report that Rab11 is required for the maintenance of cell shape via βPS integrin mediated cell adhesion. Cuticle preparations of the embryos, when Rab11 is over-expressed or activity of Rab11 is reduced via a double-stranded RNAi line, show dorsal open phenotypes. Immuno-fluorescence and immuno-histochemical analyses on embryos in the same genetic backgrounds also affect the localization of βPS integrins from the adhesion site of leading edge and amnioserosa cells during the dorsal closure stages of embryogenesis as well as the cellular morphology (cell shape) of the lateral epidermal cells.}, Keywords = {Amnioserosa, cellular morphology, cuticle, dorsal closure, Drosophila, Rab11}, volume = {1}, Number = {4}, pages = {185-190}, publisher = {Babol University of Medical Sciences}, url = {http://ijmcmed.org/article-1-60-en.html}, eprint = {http://ijmcmed.org/article-1-60-en.pdf}, journal = {International Journal of Molecular and Cellular Medicine (IJMCM)}, issn = {2251-9637}, eissn = {2251-9645}, year = {2012} } @article{ author = {shahabi, sima and Jorsaraei, Seyed Gholam Ali and Moghadamnia, Ali Akbar and Zabihi, Ebrahim and AghajanpourMir, Seyed Mohsen and MousaviKani, Seyedeh Narges and Pourbagher, Roghiyeh and Hosseini, Seyed Ahmad and Esmaili, Mohsen and Yoonesi, Ali Asghar and Zarghami, Amin and Alinezhad, Fari}, title = {Central effects of camphor on GnRH and sexual hormones in male rat}, abstract ={In Persian traditional medicine is believed that camphor (a crystalline ketone obtained from cinnamomum camphora) is a suppressor of sexual behaviors. This study examined the central effects of camphor on sexual hormones (LH, FSH and testosterone) and GnRH plasma levels in male rat. Male Wistar rats weighing 250-260gr were selected and divided into control (no treatment), sham (ICV injection of EtOH 10%) and treatment (ICV injection of camphor in three doses 4, 20, 40 µg/ 10µl in alcohol) groups. The serum samples were used for assaying of GnRH, LH, FSH and testosterone. There were no significant differences in the levels of hormones between the groups of study. Despite the central administration of camphor in hypothalamus - pituitary - gonad (HPG) axis, no significant differences were seen in sex hormone`s levels compared to the control. With this finding, it can be concluded that camphor may not effectively handle the axis via central pathway. These data recommend further studies of camphor on the HPG axis.}, Keywords = {Camphor, GnRH, LH, FSH,Testosterone, Hypothalamus - Pituitary - Gonad (H-P-G) Axis}, volume = {1}, Number = {4}, pages = {191-196}, publisher = {Babol University of Medical Sciences}, url = {http://ijmcmed.org/article-1-71-en.html}, eprint = {http://ijmcmed.org/article-1-71-en.pdf}, journal = {International Journal of Molecular and Cellular Medicine (IJMCM)}, issn = {2251-9637}, eissn = {2251-9645}, year = {2012} } @article{ author = {Ehsani, Maryam and Zabihi, Ebrahim and Gharouee, Hame}, title = {A comparison between cytotoxicity induced by two resin based sealers (2Seal and AH Plus) in Saos-2 and MG-63 cell lines}, abstract ={The aim of this study was to evaluate and compare the cytotoxicity induced by two resin-based sealers, 2Seal and AH Plus, in two osteoblast-like cell lines, MG-63 and Saos-2. Using sterile discs of both sealers in complete media, 24- and 72-h extracts were prepared. The extracts were exchanged with Saos-2 or MG-63 cell culture media at 75% confluence, and after 24 h incubation, cell viability tests were performed for each extract and cell line using MTT and trypan blue dye exclusion assays. Corresponding incubated media were used as negative control groups. For both extracts and sealers, cytotoxicity was observed in both cell lines. For Saos-2, there was no statistical difference in toxicity between the sealers for either extract (p > 0.05). For MG-63, the 2Seal 24-h extract and the AH Plus 72-h extract had greater cytotoxicity than the other extracts (p < 0.05(. Both AH Plus and 2Seal demonstrated significant cytotoxicity in these two cell lines. In contrast to 2Seal, the cytotoxicity of AH Plus in the MG-63 cell line increased with extraction time from 24 to 72 h. The AH Plus and 2Seal 24-h extracts showed different levels of cytotoxicity in the MG-63 cell line, while in the Saos-2 cell line there were no detectable differences. This may reflect higher sensitivity of the MG-63 cell line compared to Saos-2 toward cytotoxicity induced by these two sealers, or different kinetics of toxicant release from the sealers.}, Keywords = {Cytotoxicity, AH Plus, 2Seal, Osteosarcoma, Saos-2, MG-63}, volume = {1}, Number = {4}, pages = {197-202}, publisher = {Babol University of Medical Sciences}, url = {http://ijmcmed.org/article-1-63-en.html}, eprint = {http://ijmcmed.org/article-1-63-en.pdf}, journal = {International Journal of Molecular and Cellular Medicine (IJMCM)}, issn = {2251-9637}, eissn = {2251-9645}, year = {2012} } @article{ author = {Khodayari, Abbas and Ghaderian, Sayyed Mohammad Hossein and Jafarian, Mohammad and Jahangirnia, Alireza and Nayebi, Alireza and Akhlaghi, Fahimeh and Taghavi, Nasim and AkbarzadehNajar, Reza and Tabarestani, Sanaz and Khojasteh, Arash and AghabozorgAfjeh, Sarah}, title = {Evaluation of PKM2 and MAPK8IP2 polymorphism in Ameloblastic Carcinoma: A retrospective quantitative study}, abstract ={Ameloblastic carcinoma (AC) is a rare malignant epithelial odontogenic tumor that histologically retains the features of ameloblastic differentiation and exhibits cytological features of malignancy in the primary or recurrent tumor. It may develop within a preexisting ameloblastoma or arise de novo or from an odontogenic cyst. Epidemiological evidence shows that human cancer is generally caused by genotoxic factors, genes involved in the susceptibility of cancer, including those involved in metabolism or detoxification of genotoxic environment and those controlling DNA replication. Nowadays, gene polymorphism has an important role in development of malignant tumor. We report a case series study of ameloblastic carcinoma and ameloblastoma to show the role of PKM2 and MAPK8IP2 polymorphisms in these tumors. The DNA was extracted separately from specimens in paraffin sections of the tumor. Polymorphism of these genes was determined by PCR-RFLP (Polymerase Chain Reaction-Restriction fragment length polymorphism) method. The allele distributions of all samples were in Hardy-Weinberg equilibrium. The genotype and allele distribution in these genes were not statistically different between patients and controls.}, Keywords = {Ameloblastic carcinoma, PKM2, MAPK8IP2, polymorphism}, volume = {1}, Number = {4}, pages = {203-208}, publisher = {Babol University of Medical Sciences}, url = {http://ijmcmed.org/article-1-65-en.html}, eprint = {http://ijmcmed.org/article-1-65-en.pdf}, journal = {International Journal of Molecular and Cellular Medicine (IJMCM)}, issn = {2251-9637}, eissn = {2251-9645}, year = {2012} } @article{ author = {Moradian, Fatemeh and FerdosiShahandashti, Elaheh and Moulana, Zahra and Moradiankouchaksaraei, Massomeh and Asgharpour, Fariba and Mojtahedi, Ali and Rajabnia, Ramaz}, title = {Molecular detection of Integron genes and pattern of antibiotic resistance in Pseudomonas aeruginosa strains isolated from intensive care unit, Shahid Beheshti Hospital, North of Iran}, abstract ={Pseudomonas aeruginosa is one of the most important pathogens that causes nosocomial infections and shows high level of antibiotic resistance. Integrons are one of the transposable elements in bacteria and their role in antibiotic resistance has been well demonstrated. The aim of this study was a molecular characterization of the integron genes and the determination of the resistance or sensitivity pattern to ceftizoxime, ceptazidime. cephotaxim, amikacin, ofloxacin, imipenem, cefepime, ticarcillin, gentamicin, ciprofloxacin, cefazolin and ceftriaxone antibiotics in P. aeruginosa strains isolated from Intensive Care Units (ICU), Shahid Beheshti Hospital, North of Iran. This cross-sectional study was performed from 2011 to 2012. Totally, fifty four P. aeruginosa strains were isolated from ICU at Shahid-Beheshti hospital, Babol, North of Iran. The bacteria were diagnosed based on mobility, pigment production, growth in 420 C, oxidase and catalase tests. PCR analysis was carried out to detect integron genes using hep 35 and hep 36 primers. Also, disk diffusion method was performed to evaluate antibiotic susceptibility of the bacteria using ceftizoxime, ceftazidime, cephotaxime, amikacin, ofloxacin, imipenem, cefepime, ticarcillin, gentamicin, ciprofloxacin, cefazolin and ceftriaxone antibacterial reagents. This study revealed that 20 (37%) P. aeruginosa isolates had integron genes. The antibiotic susceptibility test showed that 52 (96.3%) of the isolates were multidrug-resistant. 12 out of 54 isolated bacteria were resistant to all antibiotics tested. All bacteria were resistant to cefepime (100%) and the highest resistance rate was seen to ceftazidime 92.6% fallowed by cefazolin 96.3%. The lowest resistance rate was observed to ciprofloxacin 38.9%, ofloxacin 44.4%, amikacin 46.3% and ticarcillin 48.1%. According to this study, P. aeruginosa isolates showed high level of antibiotic resistance and the presence of integrons in these strains can explain the influence of these genes in resistance creation. There was a significant association between resistance to cefotaxime, amikacin, ofloxacin, imipenem, ticarcillin, gentamicin and the presence of integrons.}, Keywords = {Pseudomonas aeruginosa, integrons, drug resistance}, volume = {1}, Number = {4}, pages = {209-216}, publisher = {Babol University of Medical Sciences}, url = {http://ijmcmed.org/article-1-56-en.html}, eprint = {http://ijmcmed.org/article-1-56-en.pdf}, journal = {International Journal of Molecular and Cellular Medicine (IJMCM)}, issn = {2251-9637}, eissn = {2251-9645}, year = {2012} } @article{ author = {Kanliada, Deniz and Coskunpinar, Ender and Orhan, Kadir Serkan and MusteriOltulu, Yasemin and Celik, Mehmet and Eren, Ayse and Yaylim, Ilhan and Değer, Kemal}, title = {Relationship of Podoplanin and Glutathione S-transferases T1 Expression with Laryngeal Cancer}, abstract ={The aim of this study is to determine whether there is a role of podoplanin and glutathione S-transferases T1 (GST-T1) expression in laryngeal squamous cell carcinoma. The study was completed with 33 patients and gene expression analysis was performed by qRT–PCR. The podoplanin and GST-T1 expression patterns were analyzed to determine their correlation with clinicopathologic parameters of laryngeal cancer. Of all patients, 20 had supraglottic, and the remaining 13 had glottic laryngeal cancer. Increased expression of podoplanin was found in 14 tumor tissues, but GST-T1 expression was not detected. Podoplanin expression did not show any prediction for regional metastasis, thyroid cartilage invasion, lymphatic vessel invasion or tumor differentiation for laryngeal cancer, also there were no significant differences in podoplanin expression between glottic and supraglottic regions, but extracapsullar extension is almost statistically significant (p=0,05).}, Keywords = {Podoplanin, GST-T1, laryngeal carcinoma, biomarker, squamous cell carcinoma}, volume = {1}, Number = {4}, pages = {217-224}, publisher = {Babol University of Medical Sciences}, url = {http://ijmcmed.org/article-1-53-en.html}, eprint = {http://ijmcmed.org/article-1-53-en.pdf}, journal = {International Journal of Molecular and Cellular Medicine (IJMCM)}, issn = {2251-9637}, eissn = {2251-9645}, year = {2012} } @article{ author = {Zarifian, Ahmadreza and Farhoodi, Zeinab and Amel, Roya and Mirzaee, Salmahe and Hassanzadeh-Nazarabad, Mohamm}, title = {Balanced Chromosomal Rearrangement in Recurrent Spontaneous Abortions: A Case Report}, abstract ={One of the major causes of spontaneous abortion before the fourth month of pregnancy is chromosomal abnormalities. We report an unusual case of a familial balanced chromosomal translocation in a consanguineous couple who experienced 4 spontaneous abortions. Chromosomal studies were performed on the basis of G-banding technique at high resolution and revealed 46, XX, t (16 6) (p12 q26) and 46, XY, t (16 6) (p12 q26) in both partners, which induced such pregnancy complications. Chromosomal balanced translocation is one of the most common causes of recurrent spontaneous abortions (RSA). In such cases prenatal diagnosis (PND) during the 16th week of gestation is strongly recommended.}, Keywords = {Chromosomal abnormality, Spontaneous abortion, Chromosomal translocation, Recurrent miscarriage, Case report}, volume = {1}, Number = {4}, pages = {225-228}, publisher = {Babol University of Medical Sciences}, url = {http://ijmcmed.org/article-1-54-en.html}, eprint = {http://ijmcmed.org/article-1-54-en.pdf}, journal = {International Journal of Molecular and Cellular Medicine (IJMCM)}, issn = {2251-9637}, eissn = {2251-9645}, year = {2012} }