@article{ author = {Mehrasa, Roya and Vaziri, Hamidreza and Oodi, Arezoo and Khorshidfar, Mona and Nikogoftar, Mahin and Golpour, Monireh and Amirizadeh, Naser}, title = {Mesenchymal Stem Cells as a Feeder Layer Can Prevent Apoptosis of Expanded Hematopoietic Stem Cells Derived from Cord Blood}, abstract ={Umbilical cord blood (UCB) has been used for transplantation in the treatment of hematologic disorders as a source of hematopoietic stem cells (HSCs). Because of insufficient number of cord blood CD34+ cells, the expansion of these cells seems to be important for clinical application. Mesenchymal stromal cells (MSCs), playing an important role in HSCs maintenance, were used as feeder layer. Apoptosis and cell cycle distribution of expanded cells were analyzed in MSCs co-culture and cytokine conditions and results were compared. Three culture conditions of cord blood HSCs were prepared ex-vivo for 14 days: cytokines (SCF, TPO and Flt3L) with MSCs feeder layer, cytokines without MSCs feeder layer and co-culture with MSCs without cytokines. Expansion was followed by measuring the total nucleated cells (TNCs), CD34+ cells and colonyforming unit (CFU) output. Flow cytometry analysis of stained cells by annexin V and propidium iodide was performed for detection of apoptosis rate and cell cycle distribution in expanded cells. Maximum cord blood CD34+ cells expansion was observed in day 10. The mean fold change of TNCs and CD34+ cells at day 10 in the co-culture system with cytokines was significantly higher than the cytokine culture without MSCs feeder layer and co-culture system without cytokines (n=6, p=0.023). The highest apoptosis rate and the least number of cells in Go/G1 phase were observed in cytokine culture without feeder layer (p=0.041). The expansion of cord blood HSCs on MSCs as a feeder layer resulted in higher proliferation and reduction in apoptosis rate.}, Keywords = {Cord blood expansion, apoptosis, co-culture, mesenchymal stem cell}, volume = {3}, Number = {1}, pages = {1-10}, publisher = {Babol University of Medical Sciences}, title_fa = {}, abstract_fa ={}, keywords_fa = {}, url = {http://ijmcmed.org/article-1-97-en.html}, eprint = {http://ijmcmed.org/article-1-97-en.pdf}, journal = {International Journal of Molecular and Cellular Medicine (IJMCM)}, issn = {2251-9637}, eissn = {2251-9645}, year = {2014} } @article{ author = {Pourghasem, Mohsen and Nasiri, Ebrahim and Shafi, Hami}, title = {Early Renal Histological Changes in Alloxan-Induced Diabetic Rats}, abstract ={Diabetes mellitus is a progressive disease. Most investigators have focused on glomerular changes in diabetic kidney and non-glomerular alterations have been less attended. The present study has been conducted to find early non-glomerular histological changes in diabetic renal tissue. Twenty male Wistar rats weighting 200-250 g were used for the diabetic group. Diabetes mellitus was induced by single injection of Alloxan. After 8 weeks, paraffin embedded blocks of kidneys were prepared for evaluating the histological changes due to diabetes. Histological study showed the deposit of eosinophilic materials in the intermediate substantial of medulla and thickening of renal arterial wall in the kidney of 70% of diabetic rats. The average weight of kidneys increased when compared to non diabetic animals. Furthermore, the amount of blood flow in arteries of all diabetic kidneys has been enhanced. The present study demonstrates some early renal histological changes in diabetes mellitus which were earlier compared to those reported previously. Diabetic nephropathy is a progressive disease and renal care design can help better prognosis achievement.}, Keywords = {Key words: Diabetes mellitus, kidney, alloxan}, volume = {3}, Number = {1}, pages = {11-15}, publisher = {Babol University of Medical Sciences}, title_fa = {}, abstract_fa ={}, keywords_fa = {}, url = {http://ijmcmed.org/article-1-116-en.html}, eprint = {http://ijmcmed.org/article-1-116-en.pdf}, journal = {International Journal of Molecular and Cellular Medicine (IJMCM)}, issn = {2251-9637}, eissn = {2251-9645}, year = {2014} } @article{ author = {Farhadi, Leila and Mohammadi-Motlagh, Hamid-Reza and Seyfi, Parivash and Mostafaie, Ali}, title = {Low Concentrations of Flavonoid - Rich Fraction of Shallot Extract Induce Delayed - Type Hypersensitivity and TH1 Cytokine IFN-gamma Expression in Balb/C Mice}, abstract ={Flavonoids are potentially immunomodulatory factors and it may be inferred that these phytochemicals contribute to immunomodulatory properties of the Allium family. In the present study, we investigated the potential mechanism underlying the immunomodulatory effect of shallot and its ethyl acetate (EA) fraction as flavonoid-rich sources. Ex vivo, effects of a hydroalcoholic extract of shallot, its fractions and quercetin on lymphocyte viability were evaluated. The proliferative effects of the fractions were examined using naive mouse lymphocytes to determine the fraction with highest impact/ activity. In addition, in a mouse model, both delayed- type hypersensitivity (DTH) responses and production of a key cytokine (interferon [IFN]-gamma) were evaluated. Both the shallot extract and its fractions inhibited lymphocytes cell growth and survival in a concentration- dependent manner. The findings also showed that the extract and especially the ethyl acetate (EA) fraction could induce lymphocyte proliferation. The evaluation of the extract and its EA fraction on DTH responses indicated that both caused a significant increase in DTH response. Furthermore, they triggered significant increases in IFNγ and decreases in interleukin (IL)-4 production by splenic mononuclear cells. Because of the significant immunomodulatory activity displayed in these studies, it is plausible that shallot could have a potential use as an immunomodulatory agent in clinical settings.}, Keywords = {Allium ascalonicum, immunomodulation, delayed-type hypersensitivity, interferon-gamma, hydroalcoh-olic extract}, volume = {3}, Number = {1}, pages = {16-25}, publisher = {Babol University of Medical Sciences}, title_fa = {}, abstract_fa ={}, keywords_fa = {}, url = {http://ijmcmed.org/article-1-127-en.html}, eprint = {http://ijmcmed.org/article-1-127-en.pdf}, journal = {International Journal of Molecular and Cellular Medicine (IJMCM)}, issn = {2251-9637}, eissn = {2251-9645}, year = {2014} } @article{ author = {TaghipourFardArdekani, Mehdi and Malekzadeh, Mahyar and VahidHosseini, Mohammad and Bordbar, Elahe and Mojtahei, Zahra and Doroudchi, Mehrnoosh and Ghaderi, Abbas}, title = {Evaluation of pre-treatment serum levels of IL-7 and GM-CSF in colorectal cancer patients}, abstract ={Background: Survival of Colorectal cancer (CRC) patients is considerably stage-dependent therefore, early diagnosis is a pivotal factor in decreasing mortality and morbidity associated with this cancer. GM-CSF and IL-7 are reported to increase in different cancers and we aim to investigate the pre-treatment serum levels of GM-CSF and IL-7 in Iranian patients with colorectal cancer. Methods: 127 patients (68 males and 59 females) before receiving chemotherapy or radiotherapy entered to this study. A control group of 50 healthy age/sex matched individuals (27 males and 23 females) were included in the study. Serum levels of GM-CSF and IL-7 were measured using commercial enzyme linked immunosorbent assays. Results: A significantly higher level of GM-CSF was found in the sera of patients with colorectal cancer compared with healthy age/sex matched controls (p=0.013). However, there was no significant difference between the levels of IL-7 in sera of patients and controls. We observed a significant elevation in the level of GM-CSF in poorly differentiated tumors (p=0.024). Also a significant correlation between lymphatic invasion and the level of GM-CSF in sera of CRC patients was detected (p=0.01). We found an increase in the level of IL-7 in the three patients in the moderate stages of the tumor concomitant with a decrease in the level of GM-CSF. Conclusion: Increase in the level of GM-CSF is accompanied by CRC progression in Iranian patients, while there may be a potential therapeutic effect of IL-7 in this disease.}, Keywords = {Colorectal cancer, Serum, GM-CSF, IL-7 }, volume = {3}, Number = {1}, pages = {26-34}, publisher = {Babol University of Medical Sciences}, title_fa = {}, abstract_fa ={}, keywords_fa = {}, url = {http://ijmcmed.org/article-1-112-en.html}, eprint = {http://ijmcmed.org/article-1-112-en.pdf}, journal = {International Journal of Molecular and Cellular Medicine (IJMCM)}, issn = {2251-9637}, eissn = {2251-9645}, year = {2014} } @article{ author = {Ghaffari, Salman and Kalantari, Narges and Hart, Charles A}, title = {A Multi-locus Study for Detection of Cryptosporidium Species Isolated from Calves Population, Liverpool UK}, abstract ={Cryptosporidium is an obligate intracellular protozoan parasite infecting a wide range of hosts. The current study investigated the genetic profile of Cryptosporidium species in calves in Liverpool, England. Fifty-two calve fecal samples were collected from a farm and initially screened by Auramine phenol, modified Ziehl-Neelsen and ELISA. PCR analysis of 18S rRNA gene was carried out for the positive samples. Then, positive PCR samples were genotyped by an 18S rRNA- based PCR-RFLP, COWP - based PCR- RFLP PCR of GP60 and HSP70 genes. Additionally, sequence analysis was carried out based on representative isolates of four loci. Cryptosporidium oocysts and antigens were detected in 34 out if 52 (65.4%) samples using screening techniques. Genotype analysis showed the presence of C. hominis and C. parvum in one and thirteen samples, respectively. Furthermore, subtypes of C. hominis Ib, C. parvum IIa C. parvum subtype 2 were identified by GP60 and HSP70 sequences, respectively. These findings indicate the diversity of the molecular characteristics of Cryptosporidium species in calves’ isolates. Moreover, referring to the literature we report two new subtypes of C. parvum IIa and a rare case of C. hominis Ib in calves population.}, Keywords = {Cryptosporidium, Calves, Sub-type, UK}, volume = {3}, Number = {1}, pages = {35-42}, publisher = {Babol University of Medical Sciences}, title_fa = {}, abstract_fa ={}, keywords_fa = {}, url = {http://ijmcmed.org/article-1-121-en.html}, eprint = {http://ijmcmed.org/article-1-121-en.pdf}, journal = {International Journal of Molecular and Cellular Medicine (IJMCM)}, issn = {2251-9637}, eissn = {2251-9645}, year = {2014} } @article{ author = {Motalleb, Gholamrez}, title = {Listeria Monocytogenes La111 and Klebsiella Pneumoniae KCTC 2242: Shine-Dalgarno Sequences}, abstract ={Listeria monocytogenes can cause serious infection and recently, relapse of listeriosis has been reported in leukemia and colorectal cancer, and the patients with Klebsiella pneumoniae are at increased risk of colorectal cancer. Translation initiation codon recognition is basically mediated by Shine-Dalgarno (SD) and the anti-SD sequences at the small ribosomal RNA (ssu rRNA). In this research, Shine-Dalgarno sequences prediction in Listeria monocytogenes La111 and Klebsiella pneumoniae KCTC 2242 was investigated. The whole genomic sequence of Listeria monocytogenes La111 and Klebsiella pneumoniae KCTC 2242 were retrieved from http://www.ncbi.nlm.nih.gov/ (Listeria monocytogenes La111 NCBI Reference sequence: NC_020557 Klebsiella pneumoniae KCTC 2242 NCBI Reference sequence: CP002910) in order to be analyzed with DAMBE software and BLAST. The results showed that the consensus sequence for Klebsiella pneumoniae KCTC 2242 was CCCCCCCUCCCCCUCCCCCUCCUCCUCCUUUUUAAAAAAGGGGAAAAACC and for Listeria monocytogenes La111 was CCCCCCCUCCCCCUUUCCCUCCUAUUCUUAUAAAAGGGGG-GGGGUUCAC. The PSD was higher in Listeria monocytogenes La111 compared to Klebsiella pneumoniae KCTC 2242 (0.9090> 0.8618). The results showed that Nm in Listeria monocytogenes La111 was higher than Klebsiella pneumoniae KCTC 2242 (4.5846> 4.4862). Accurate characterization of SD sequences may increase our knowledge on how an organism’s transcriptome is related to its cellular proteome.}, Keywords = {Molecular biology, genomics, microbiology, Shine-Dalgarno sequences}, volume = {3}, Number = {1}, pages = {43-50}, publisher = {Babol University of Medical Sciences}, title_fa = {}, abstract_fa ={}, keywords_fa = {}, url = {http://ijmcmed.org/article-1-119-en.html}, eprint = {http://ijmcmed.org/article-1-119-en.pdf}, journal = {International Journal of Molecular and Cellular Medicine (IJMCM)}, issn = {2251-9637}, eissn = {2251-9645}, year = {2014} } @article{ author = {Bahari, Pejman and Salehi, Mitra and Seyyedabadi, Mohsen and Mohammadi, Ahm}, title = {Molecular Identification of Macroscopic And Microscopic Cysts of Sarcocystis in Sheep in North Khorasan Province, Iran}, abstract ={Sarcocystis is an obligatory intracellular protozoan parasite which can infect humans and animals. Sheep are intermediate hosts of four Sarcocystis species: Sarcocystis tenella, Sarcocystis gigantea, Sarcocystis arieticanis, and Sarcocystis medusiformision. The purpose of the study was to determine the molecular identification of the macroscopic and microscopic cysts of Sarcocystis in sheep. In this investigation, we assessed the macroscopic and microscopic cysts of Sarcocystis in sheep carcasses. The digestion method was used for observing of bradyzoite in heart, liver, diaphragm and muscle samples. PCR Analysis was conducted on macroscopic and microscopic cysts and also all other samples. Sequencing was done for ten PCR products. Genotypes were identified by Blast search and homology analysis. Macrocyst was seen in two muscle tissues. Digested method and PCR analysis were positive in all samples (heart, liver, diaphragm muscle). Genotyping of ten tissues samples proved that the genotype of macroscopic and microscopic cysts belonged to Sarcocystis gigantea and Sarcocystis tenella, respectively. Microscopic cysts are more prevalent than macroscopic cysts and they can cause enormous economic losses.}, Keywords = {Sarcocystis tenella, Sarcocystis gigantea, Molecular analysis}, volume = {3}, Number = {1}, pages = {51-56}, publisher = {Babol University of Medical Sciences}, title_fa = {}, abstract_fa ={}, keywords_fa = {}, url = {http://ijmcmed.org/article-1-117-en.html}, eprint = {http://ijmcmed.org/article-1-117-en.pdf}, journal = {International Journal of Molecular and Cellular Medicine (IJMCM)}, issn = {2251-9637}, eissn = {2251-9645}, year = {2014} } @article{ author = {Assadi, Najmeh and Zabihi, Ebrahim and Khosravifarsani, Meysam and Khafri, Soraya and AkhavanNiaki, Haleh and Amiri, Mehrangiz and ShabestaniMonfared, Ali}, title = {Radioadaptive Response in Human Lymphocyte Cells}, abstract ={The adaptive response (AR) is a phenomenon by which cells exposure to sublethal doses of DNA-damaging agents (non-mutagenic dose of chemical or radiation), known as conditioning treatment (CT), leads to increased resistance to a subsequent exposure to a higher dose of the same or other agents, known as challenge treatment (CR). The adaptive response (AR) induced by radiation in human lymphocytes has been reported in a range of 1-20cGy pre-exposure. In this study, we investigated the adaptive response using 5cGy conditioning dose of gamma rays followed by 2 Gy challenging dose in peripheral human lymphocyte cells. Blood samples were taken from 30 female volunteers and this experiment was carried out by delivering 5 cGy gamma radiation followed by 2 Gy of challenging. Consequently, the number of micronuclei (MN) in binuclear lymphocyte cells was counted as an endpoint. The results showed that the mean frequency of micronuclei in binuclear lymphocytes which have received both conditioning and challenge doses are significantly reduced in comparison to those only exposed to 2 Gy (20.46±2.13, 30.2±3.29) (P< 0.01). The results showed the existence of an in vitro adaptive response in lymphocyte cell exposed to low dose of gamma radiations.}, Keywords = {Adaptive Response (AR), challenge treatment (CR), condition treatment (CT), micronuclei assay}, volume = {3}, Number = {1}, pages = {57-60}, publisher = {Babol University of Medical Sciences}, title_fa = {}, abstract_fa ={}, keywords_fa = {}, url = {http://ijmcmed.org/article-1-107-en.html}, eprint = {http://ijmcmed.org/article-1-107-en.pdf}, journal = {International Journal of Molecular and Cellular Medicine (IJMCM)}, issn = {2251-9637}, eissn = {2251-9645}, year = {2014} }