@article{ author = {Wannapruk, Pattharin and Deesrisak, Kamolchanok and Roytrakul, Sittiruk and Tanyong, Dali}, title = {Sesamin Acts as Anti-leukemic Compound Interacted with Novel Phosphoprotein Targets and Induced Apoptosis in Leukemic Cells}, abstract ={Leukemia is one of the high-incidence cancers that characterized by an abnormal production of immature white blood cells. Subjected to many reports on side effects of conventional chemotherapy, herbs and natural compounds have been studied as an alternative medicine. This study, sesamin, a lignan in sesame seed that has pharmaceutical functions including anti-cancer, was chosen and treated with MOLT-4 and NB4 leukemic cell lines in various concentrations for 24 and 48 hours. The effect of sesamin on cell inhibition and expression levels of apoptotic genes in leukemic cell lines were investigated by MTT assay and real-time PCR, respectively. Moreover, apoptotic proteins were studied by mass spectrometry and bioinformatics tools to investigate the relation between sesamin and targeted proteins. Results showed that sesamin increased cell inhibition in both cell lines in dose- and time-dependent manner. Levels of caspase-3, -7, -8, and -9 gene expressions were significantly increased, while BCL-2 was decreased drastically in sesamin-treated cells. From bioinformatics study, PARP4, IPPK and caspase family proteins were found to be involved in sesamin induced apoptosis in leukemic cells. Besides, doxorubicin, a chemotherapeutic drug, also shared the same protein targets as sesamin in apoptosis pathway. Sesamin demonstrates its potential to enhance cell inhibition and promote cell apoptosis in both MOLT-4 and NB4 leukemic cell lines. This study would be benefit for the development of sesamin as an effective anti-leukemia drug in the future.  }, Keywords = {Sesamin, leukemia, MOLT-4, NB4, apoptosis, Doxorubicin}, volume = {11}, Number = {1}, pages = {1-15}, publisher = {Babol University of Medical Sciences}, title_fa = {}, abstract_fa ={}, keywords_fa = {}, doi = {10.22088/IJMCM.BUMS.11.1.1}, url = {http://ijmcmed.org/article-1-1886-en.html}, eprint = {http://ijmcmed.org/article-1-1886-en.pdf}, journal = {International Journal of Molecular and Cellular Medicine (IJMCM)}, issn = {2251-9637}, eissn = {2251-9645}, year = {2022} } @article{ author = {Kiyamehr, Pegah and Shahidi, Minoo and Samii, Amir and Zaker, Farh}, title = {Dual Effects of Resveratrol on the Expression and Secretion of Angiogenic Factors}, abstract ={Angiogenesis is an essential process in the growth, development and transition of tumors from dormancy to proliferating state. Resveratrol (RSV), as a natural polyphenolic compound, is claimed to be effective in angiogenesis regulation. The aim of this study was to evaluate the impact of RSV on angiogenesis process in human umbilical vein endothelial cells (HUVECs) alone and co-cultured with Jurkat cells. The effects of RSV on HUVECs and Jurkat cells viability and apoptosis were measured by MTT and annexin-V/PI methods. HUVECs were co-cultured with pre-treated Jurkat cells and incubated for 24-72 h. Angiogenesis was also investigated by analyzing the expression of VEGF, VEGFR-2, and IL-8 employing qPCR and ELISA. Treatment with Jurkat cells had no significant effect on apoptosis of HUVECs at low concentrations of RSV (20-40 µM), but higher concentrations (80-160 µM) increased apoptosis. Although RSV significantly reduced VEGFR2 and IL-8 gene expression as well as IL-8 protein concentration in HUVECs, this effect was reversed in the Jurkat-treated HUVECs in the co-culture model. The expression of VEGF in Jurkat cells significantly increased following treatment with RSV. Therefore, RSV had a direct antiangiogenic effect on HUVECs. Unexpectedly its indirect effect was not significant on Jurkat-treated HUVECs in co-culture conditions. The results of our experiments highlight that although RSV may be effective in antiangiogenesis therapy, in some conditions it may favor angiogenesis. Therefore, appropriate concentrations should be achieved to minimize the possible unpredicted effects of RSV.}, Keywords = {Angiogenesis, resveratrol, co-culture, VEGFR2, VEGF, IL-8}, volume = {11}, Number = {1}, pages = {16-30}, publisher = {Babol University of Medical Sciences}, title_fa = {}, abstract_fa ={}, keywords_fa = {}, doi = {10.22088/IJMCM.BUMS.11.1.16}, url = {http://ijmcmed.org/article-1-1830-en.html}, eprint = {http://ijmcmed.org/article-1-1830-en.pdf}, journal = {International Journal of Molecular and Cellular Medicine (IJMCM)}, issn = {2251-9637}, eissn = {2251-9645}, year = {2022} } @article{ author = {Dadashzadeh, Elnaz and SaghaeianJazi, Marie and Abdolahi, Nafiseh and Mohammadi, Saeed and Saeidi, Mohse}, title = {Comparison of a Suggested Model of Fibrosis in Human Dermal Fibroblasts by Serum from Systemic Sclerosis Patients with Transforming Growth Factor β Induced in vitro Model}, abstract ={Systemic sclerosis (SSc) is a chronic autoimmune disease, featuring fibrosis in multiple organs. The serum from SSc patients contain inflammatory mediators, contributing to SSc pathogenesis and could be used to develop cell culture models. Here, we compared the fibrotic effects of serum samples from SSc patients with TGFβ1 on human dermal fibroblasts (HDFs). HDF cells were cultured in four different culture media supplementations; 10% SSc serum, 10% healthy human serum, 10% fetal bovine serum  or 10% FBS supplemented with 10 ng/ml human TGFβ. The collagen content in cell layers was measured by spectrophotometric Picro-Sirius red staining. The mRNA expression of α-SMA, COL I and III, TGFβ1, arginase and E-Cadherin genes were determined by real time RT-PCR. TGF-β1 levels in cell culture supernatants were measured using ELISA. Cell layer collagen content was significantly increased following TGF-β1 treatment, compared with FBS group and SSc serum treatment in comparison with healthy controls. Although not statistically significant, the mRNA expression of α-SMA, COLI and III, TGFβ1, and arginase increased upon TGF-β1 treatment in comparison with FBS group, and in SSc serum treatment group in comparison with healthy controls. E-Cadherin decreased following TGF-β1 treatment and SSc serum treatment in comparison with their counterparts. TGF-β1 levels increased in cell culture supernatants of HDF cells exposed to TGF-β1 and SSc serum. An in vitro model of SSc serum-induced fibrosis using human HDF cells was evaluated in comparison to the TGF-β1 fibrosis induced model and data suggested that it may be used in documenting the role of pro-fibrotic factors in serum or plasma from SSc patients.}, Keywords = {Fibrosis, human dermal fibroblast, systemic sclerosis, autoimmune disease, TGF-β}, volume = {11}, Number = {1}, pages = {31-40}, publisher = {Babol University of Medical Sciences}, title_fa = {}, abstract_fa ={}, keywords_fa = {}, doi = {10.22088/IJMCM.BUMS.11.1.31}, url = {http://ijmcmed.org/article-1-1794-en.html}, eprint = {http://ijmcmed.org/article-1-1794-en.pdf}, journal = {International Journal of Molecular and Cellular Medicine (IJMCM)}, issn = {2251-9637}, eissn = {2251-9645}, year = {2022} } @article{ author = {Zarinfard, Giti and Aliakbari, Maryam and Asgari, Vajihe and Razavi, Shahnaz}, title = {Upregulation of Neurotrophic Factors and Myelin Basic Protein in Schwann-like Cells by T3 Hormone Following Transdifferentiation of Human Adipose-derived Stem Cells}, abstract ={Peripheral nerve regeneration is a complicated phenomenon. Thyroid hormones are known as critical regulators in the nervous system development. The Schwann cells have the regenerative potency in the peripheral nervous system. In this study, the human adipose-derived stem cells were assessed in vitro, for transdifferentiation potency into Shwann-like cells (SLCs) as a candidate source for clinical cell therapy, under the treatment of triiodothyronine (T3) hormone, and compared with the untreated cells. The cell viability rate, myelination and neurotrophic factors expression of SLCs were evaluated two weeks post- induction by MTT assay, immunocytochemistry and real-time RT-PCR techniques, respectively. The obtained results revealed a significant decrease in SLCs viability, compared to the adipose-derived stem cells (P < 0.001). Immunocytochemistry technique was applied to detect SLCs markers, such as S100β, GFAP and myelin basic proteins (MBP) in the presence and absence of T3 treatment. The results indicated that administering T3 can significantly increase the differentiation and myelination potency of SLCs (P < 0.01). The findings of real-time RT-PCR technique indicated that the expression of Schwann cells markers, MBP, brain-derived neurotrophic factor and glial cell-derived neurotrophic factor were upregulated significantly with T3 hormone administration in comparison with the untreated cells (P < 0.05). The SLCs were able to express neurotrophic factors and myelination related genes in the presence of T3 hormone.  Therefore, T3 administration improved myelination potency of adipose-derived stem cells, in vitro. Further in vivo experiments are necessary to confirm the advantages of using a combination of autologous SLCs and T3 hormone for peripheral nerve injury recovery.}, Keywords = {Adipose-derived stem cells, myelin, neurotrophic factors, Schwann cells, thyroid hormone}, volume = {11}, Number = {1}, pages = {41-54}, publisher = {Babol University of Medical Sciences}, title_fa = {}, abstract_fa ={}, keywords_fa = {}, doi = {10.22088/IJMCM.BUMS.11.1.41}, url = {http://ijmcmed.org/article-1-1692-en.html}, eprint = {http://ijmcmed.org/article-1-1692-en.pdf}, journal = {International Journal of Molecular and Cellular Medicine (IJMCM)}, issn = {2251-9637}, eissn = {2251-9645}, year = {2022} } @article{ author = {Najafipour, Reza and Mohammadi, Davood and Momeni, Mabood and Moghbelinejad, Sahar}, title = {ACE-2 Expression and Methylation Pattern in Bronchoalveolar Lavage Fluid and Bloods of Iranian ARDS Covid-19 Patients}, abstract ={The aim of the present study was to investigate the expression and methylation pattern of the angiotensin I converting enzyme 2 (ACE-2) in acute respiratory distress syndrome (ARDS) covid-9 patients. A total of 25 patients with covid-19 ARDS and 20 controls were recruited. Expression of the ACE-2 gene was evaluated by quantitative real time PCR, and methylation of CpG dinucleotides, in the ACE-2 promoter, was quantified using bisulfite pyro-sequencing. Our results showed high expression of the ACE-2 gene in the blood samples of ADRS patients (1.93± 0.67) in comparison to controls (0.62±0.35) (p = 0.03). Correspondingly, in ARDS Bronchoalveolar lavage fluid (BALF) samples, there was a high expression of this gene (1.8±0.78) in comparison to controls (0.58±0.2) (p <0.05). Moreover, the methylation rate of the ACE-2 gene in blood samples of ARDS patients was 64.07 ±6.1 in comparison to controls (80.3 ±7.3) (p<0.0001).  In BALF samples, there was this pattern too (55.07 ±3.1 vs. 72.35±5.1) (p<0.0001). Finally, a significant correlation was found between expression and methylation in BALF (R= -0.54, P= 0.002) and blood (R= -0.321, P= 0.013) samples. These results indicated that aberrant methylation of the ACE-2 promoter might be associated with high expression of this gene and the occurrence of ARDS in covid-19 patients.  }, Keywords = {Keywords: Covid-19, ACE-2, expression, methylation}, volume = {11}, Number = {1}, pages = {55-63}, publisher = {Babol University of Medical Sciences}, title_fa = {}, abstract_fa ={}, keywords_fa = {}, doi = {10.22088/IJMCM.BUMS.11.1.55}, url = {http://ijmcmed.org/article-1-1867-en.html}, eprint = {http://ijmcmed.org/article-1-1867-en.pdf}, journal = {International Journal of Molecular and Cellular Medicine (IJMCM)}, issn = {2251-9637}, eissn = {2251-9645}, year = {2022} } @article{ author = {SaberAmoli, Sagahr and Hasanzadeh, Ali and Sadeghi, Farzin and Chehrazi, Mohammad and Seyedmajidi, Maryam and Zebardast, Arghavan and Yahyapour, Yousef}, title = {Prevalence of Co-infection by Human Papillomavirus, Epstein-Barr Virus and Merkel Cell Polyomavirus in Iranian Oral Cavity Cancer and Pre-malignant Lesions}, abstract ={Human papillomavirus (HPV) is recognized as a most important risk factor in oral cavity cancer and pre-malignant lesions; however, etiological association of concomitant infection with other oncogenic viruses as a co-factor has not been definitively proven. The present study aimed to determine the prevalence of co-infection with HPV, Epstein–Barr virus (EBV) and Merkel Cell PolyomaVirus (MCPyV) in oral cavity lesions in Iranian patients. One hundred and fourteen oral cavity samples, including 33 oral squamous cell carcinoma, 28 oral lichen planus, 16 oral epithelial dysplasia and 37 oral irritation fibromas were analyzed for the HPV, EBV and MCPyV infection by quantitative real-time PCR. According to histological features 32.5% and 28.9% of cases were oral irritation fibroma and oral squamous cell carcinoma, respectively. Infection with at least two viruses was detected in 21.1% of patients. In this group, co-infection with HPV/EBV was identified in 37.5% of cases, HPV/MCPyV in 29.2%, EBV/MCPyV in 12.5%, and HPV/EBV/MCPyV in 20.8%. There was no statistically significant difference between multiple infections and anatomical locations of cancer. The prevalence of triple viral infection (HPV/EBV/MCPyV) in well differentiated tumors was higher than EBV or MCPyV single infection. This study revealed that co-infection of HPV, EBV and MCPyV can be detected in both malignant and non-malignant oral cavity tissues, and co-infection with all three viruses in well differentiated tumors can be shown as a synergistic hypothesis of the pathogenic role of these viruses in oral malignant transformation.  }, Keywords = {Co-infection, human papillomavirus (HPV), Merkel cell polyomavirus (MCPyV), Epstein–Barr virus (EBV), oral lesions}, volume = {11}, Number = {1}, pages = {64-77}, publisher = {Babol University of Medical Sciences}, title_fa = {}, abstract_fa ={}, keywords_fa = {}, doi = {10.22088/IJMCM.BUMS.11.1.64}, url = {http://ijmcmed.org/article-1-1873-en.html}, eprint = {http://ijmcmed.org/article-1-1873-en.pdf}, journal = {International Journal of Molecular and Cellular Medicine (IJMCM)}, issn = {2251-9637}, eissn = {2251-9645}, year = {2022} } @article{ author = {Sadeghi-Zadeh, Maryam and HomayouniMoghadam, Farshad and NasrEsfahani, Mohammad Hossei}, title = {Ferulic Acid Induces NURR1 Expression and Promotes Dopaminergic Differentiation in Neural Precursor Cells}, abstract ={Degeneration of dopaminergic (DA) neurons in the substantia nigra is known as the main cause of Parkinson's disease (PD). Preventing the loss of DA neurons alongside the cell-replacement therapy have brought tremendous hope for the treatment of PD. For this purpose, various studies have been done to find specific DA neuro-protective compounds or progressing DA-differentiation methods. Ferulic acid (FA) has strong neuro-protective effects, but at this point its role on protection and differentiation of DA neurons is not well-defined. Mouse neural stem cells (mNSCs) were treated with FA and expressions of TH (tyrosine hydroxylase) and NURR1 as the DA neuron specific markers were determined using real time qRT-PCR and immunostaining assays. Finally, efficacy of FA on DA differentiation was evaluated in comparison with other methods using fibroblast growth factor 8b (FGF8b) and sonic hedgehog (SHH). Treatment with FA could increase the Th and Nurr1 gene expressions in mNSCs. Also, it enhanced β-tubullin-III expression and increased the neurite length in treated groups. Real time qRT-PCR and immunostaining assays showed that FA could increase DA differentiation in mNSCs effectively. Also, gene expression profile in some groups showed that FA can raise the differentiation rate of other neuronal subtypes such as cholinergic neurons. FA effectively induces the DA differentiation in neural precursor cells by its ability to increase the expression of the NURR1 transcription factor, which is a known transcription factor for differentiation of midbrain DA neurons.  }, Keywords = {Dopaminergic, neural stem cell, Ferulic acid, differentiation, Nurr1, Tyrosine hydroxylase}, volume = {11}, Number = {1}, pages = {78-87}, publisher = {Babol University of Medical Sciences}, title_fa = {}, abstract_fa ={}, keywords_fa = {}, doi = {10.22088/IJMCM.BUMS.11.1.78}, url = {http://ijmcmed.org/article-1-1891-en.html}, eprint = {http://ijmcmed.org/article-1-1891-en.pdf}, journal = {International Journal of Molecular and Cellular Medicine (IJMCM)}, issn = {2251-9637}, eissn = {2251-9645}, year = {2022} }