@article{ author = {Bressan, Eriberto and Botticelli, Daniele and Sivolella, Stefano and Bengazi, Franco and Guazzo, Riccardo and Sbricoli, Luca and Ricci, Sara and Ferroni, Letizia and Gardin, Chiara and UrbizoVelez, Joaquin and Zavan, Barbar}, title = {Adipose- Derived Stem Cells as a Tool for Dental Implant Osseointegration: an Experimental Study in the Dog}, abstract ={The biological interaction between the jaw bones and dental implant is fundamental for the long-term success of dental implant placement. Nevertheless, the insufficient bone volume remains a major clinical problem, especially in case of immediate dental implant. Using a canine model, the present study proves the regenerative potential of adipose- derived stem cells (ADSCs) to repair peri-implant bone defects occurring in immediate dental implant placement. In six labradors, all mandibular premolars and the first molars were extracted bilaterally and three months later dental implants were installed with a marginal gap. The marginal defects were filled with hydroxyapatite (HA)-based scaffolds previously seeded with ADSCs. After one month of healing, specimens were prepared for histological and histomorphometric evaluations. Histological analyses of ground sections show that ADSCs significantly increase bone regeneration. Several new vessels, osteoblasts and new bone matrix were detected. By contrast, no inflammatory cells have been revealed. ADSCs could be used to accelerate bone healing in peri- implant defects in case of immediate dental implant placement.}, Keywords = {Adipose- derived stem cells, alveolar bone loss, peri- implant bone defect, immediate dental implant, bone regeneration, hydroxyapatite}, volume = {4}, Number = {4}, pages = {197-208}, publisher = {Babol University of Medical Sciences}, url = {http://ijmcmed.org/article-1-358-en.html}, eprint = {http://ijmcmed.org/article-1-358-en.pdf}, journal = {International Journal of Molecular and Cellular Medicine (IJMCM)}, issn = {2251-9637}, eissn = {2251-9645}, year = {2015} } @article{ author = {Fazeli, Zahra and Rajabibazl, Masoumeh and Salami, Siamak and VazifehShiran, Nader and Ghaderian, Sayyed Mohammad Hossein and Omrani, Mir Davoo}, title = {Expression Pattern of Neuronal Markers in PB-MSCs Treated by Growth Factors Noggin, bFGF and EGF}, abstract ={Mesenchymal stem cells (MSCs) have the ability to differentiate into neuronal like cells under appropriate culture condition. In this study, we investigated whether MSCs derived from human peripheral blood (PB-MSCs) can differentiate into neuronal like cells by synergic effect of the growth factors EGF, bFGF and Noggin. For this purpose, the expression of five neuronal markers (Nestin, ;beta III tubulin, NFM, MAP2 and NSE) were evaluated in treated PB-MSCs by SYBR Green Real time PCR. The expression analysis showed a higher expression of ;beta- tubulin and NFM in treated BP-MSCs compared with untreated PB-MSCs as a control group. The expression of Nestin was also diminished in PB-MSCs treated with Noggin. This study suggested that the treatment of PB- MSCs with Noggin alongside with bFGF and EGF might differentiate these cells into neuronal lineage cells. The obtained results could be further developed for useful applications in regenerative medicine.}, Keywords = {Mesenchymal Stem Cells, Differentiation, Neuronal markers, Noggin}, volume = {4}, Number = {4}, pages = {209-217}, publisher = {Babol University of Medical Sciences}, url = {http://ijmcmed.org/article-1-384-en.html}, eprint = {http://ijmcmed.org/article-1-384-en.pdf}, journal = {International Journal of Molecular and Cellular Medicine (IJMCM)}, issn = {2251-9637}, eissn = {2251-9645}, year = {2015} } @article{ author = {Mahdavi, Mehdi and Ebtekar, Massoumeh and Hassan, Zuhair Mohammad and Faezi, Sobhan and KhorramKhorshid, Hamidreza and Taghizadeh, Morteza and Azadmanesh, Keyh}, title = {An HIV-1 Mini Vaccine Induced Long-lived Cellular and Humoral Immune Responses}, abstract ={Memory formation is the most important aspect of a vaccine which can guarantee long-lasting immunity and protection. The main aim of the present study was to evaluate the memory immune responses after immunization with a mini vaccine. Mice were immunized with human immunodeficiency virus-1 P24-Nef fusion peptide and then cellular and humoral immune responses were evaluated. In order to determine long-lived memory, immune responses were monitored for 20 weeks after final immunization. The results showed that the candidate vaccine induced proliferation and cytotoxic T lymphocyte responses and shifted cytokine patterns to T helper-1 profile. Evaluation of humoral immune responses also showed an increase in total peptide specific-IgG titer and a shift to IgG2a humoral response. Monitoring of immune responses at weeks 4, 12 and 20 after last immunization showed that immunologic parameters have been sustained for 20 weeks. Our findings support the notion that long-lived memory responses were achieved using a mini vaccine immunization.}, Keywords = {BALB/c mice, HIV-1, long-lived immune responses, P24-Nef fusion peptide }, volume = {4}, Number = {4}, pages = {218-226}, publisher = {Babol University of Medical Sciences}, url = {http://ijmcmed.org/article-1-390-en.html}, eprint = {http://ijmcmed.org/article-1-390-en.pdf}, journal = {International Journal of Molecular and Cellular Medicine (IJMCM)}, issn = {2251-9637}, eissn = {2251-9645}, year = {2015} } @article{ author = {Shahi, Heshmat and Reiisi, Somayeh and Bahreini, Rasol and Bagheri, Nader and Salimzadeh, Loghman and Shirzad, Hedayatollah}, title = {Association between Helicobacter pylori cagA, babA2 Virulence Factors and Gastric Mucosal Interleukin-33 mRNA Expression and Clinical Outcomes in Dyspeptic Patients}, abstract ={Helicobacter pylori (H. pylori) infection has been reported in more than half of the world human population. It is associated with gastric inflammation and noticeable infiltration of the immune cells to the stomach mucosa by several cytokines secretion. IL-1;beta, IL-18 have been shown to contribute to H. pylori induced gastritis, but the details of inflammation and association of virulence factors remain unclear. IL-1 cytokine family has a new additional cytokine, Interleukin-33 (IL-33), which is contemplated to have an important role for host defense against microorganisms. H. pylori virulence factors important in gastritis risk are the cag pathogenicity island (cag-PAI) and babA. This study evaluated IL-33 mucosal mRNA expression levels in infected and uninfected patients and its relationship with bacterial virulence factors cagA, babA2 and type of gastritis. Total RNA was extracted from gastric biopsies of 79 H. pylori-infected patients and 51 H. pylori-negative patients. Mucosal IL-33 mRNA expression levels in gastric biopsies were assessed using real-time PCR. Existence of virulence factors were detected by PCR. IL-33 mRNA expression was significantly higher in biopsies of H. pylori-infected patients compared to H. pylori-uninfected patients (P<0.0001). Also there was a direct relationship between virulence factor bab-A2 and enhancement in IL-33 mRNA expression. Furthermore, IL-33 mRNA expression level was significantly lower in chronic gastritis patients compared with patients with active gastritis (P<0.001). IL-33 may play a crucial role in the inflammatory response and induction of the chronic gastritis and severity of inflammatory changes in the gastric mucosa.}, Keywords = {Helicobacter pylori, gastritis, interleukin-33, virulence factor}, volume = {4}, Number = {4}, pages = {227-234}, publisher = {Babol University of Medical Sciences}, url = {http://ijmcmed.org/article-1-423-en.html}, eprint = {http://ijmcmed.org/article-1-423-en.pdf}, journal = {International Journal of Molecular and Cellular Medicine (IJMCM)}, issn = {2251-9637}, eissn = {2251-9645}, year = {2015} } @article{ author = {Mahdavipoor, Behroz and Asgharzadeh, Mohammad and Fallah, Esmaeel and Hatam-Nahavandi, Kareem and Rashedi, Jalil and Dalimi, Abdolhossei}, title = {Molecular Characterization of Cryptosporidium Species in Children with Diarrhea in North West of Iran}, abstract ={Cryptosporidium is one of the most common causes of childhood diarrhea in developing countries. The aim of this randomized pilot study was to detect and characterize infective species and determine the genotypes of Cryptosporidium parasites in pediatric patients suffering from diarrhea in North West of Iran. A total of 113 fecal samples were collected from diarrheic children hospitalized in Tabriz Pediatric Hospital. The amplification of small subunit ribosomal RNA gene was performed using a nested polymerase chain reaction protocol and its products were digested using two restriction enzymes for Cryptosporidium species and genotype differentiation. Cryptosporidium oocysts were found in 2 (1.76%) children with diarrhea and restriction pattern revealed the presence of C.parvum bovine genotype in both positive fecal samples. The findings indicate that Cryptosporidium parvum is responsible for cryptosporidiosis in children in the study region and probably zoonotic transmission is the predominant route of parasite transmission.}, Keywords = {Child, Cryptosporidium, genotype, PCR, Iran}, volume = {4}, Number = {4}, pages = {235-239}, publisher = {Babol University of Medical Sciences}, url = {http://ijmcmed.org/article-1-382-en.html}, eprint = {http://ijmcmed.org/article-1-382-en.pdf}, journal = {International Journal of Molecular and Cellular Medicine (IJMCM)}, issn = {2251-9637}, eissn = {2251-9645}, year = {2015} } @article{ author = {HasannejadBibalan, Meysam and Eshaghi, Morteza and Sadeghi, Javad and Asadian, Mahla and Narimani, Tahmineh and Talebi, Malihe}, title = {Clonal Diversity in Multi Drug Resistant (MDR) Enterococci Isolated from Fecal Normal Flora}, abstract ={Enterococci are Gram positive and catalase- negative cocci that are found in the gastrointestinal tract of mammals and birds, and are readily isolated from soil, surface and waters. The aim of this study was to discriminate between Enterococcus isolates based on repetitive element sequence based –PCR (Rep-PCR) with the BOXA2R primer and their antibiotics profile. Enterococci isolates were obtained from 180 fecal samples. The isolates were identified by biochemical reaction and specific identification was confirmed by PCR with species specific primers. All isolates were subjected to Rep typing and antimicrobial susceptibility tests. Rep-PCR analysis of 180 isolates revealed 93 REP types with forty-five single types (ST1 to ST45) and forty-eight common types (CT1 to 48). Antibiotic susceptibility tests exhibited that  53 (29.4%), 43 (23.8%), 11 (6.1%) and 9 (5%) were resistant to erythromycin, tetracycline, gentamicin and ciprofloxacin  respectively but among the isolates, sixteen were multi drug resistant (MDR). These MDR isolates showed 11 Rep types with seven single types and four common types. In addition, 81.2% of MDR isolates were from male subjects and the average age of these persons was more than fifty years. This study showed that 56.2% of MDR isolates were homogeneous with 95 % similarity, and high rate of resistance to tetracycline and erythromycin (81.2%) were observed in these isolates. The concern about these normal flora isolates are the pathogenic potential of these bacteria through the horizontal transfer of antibiotic resistance and virulence genes.}, Keywords = {Enterococcus, Rep-PCR, antibiotic profile, normal flora}, volume = {4}, Number = {4}, pages = {240-244}, publisher = {Babol University of Medical Sciences}, url = {http://ijmcmed.org/article-1-370-en.html}, eprint = {http://ijmcmed.org/article-1-370-en.pdf}, journal = {International Journal of Molecular and Cellular Medicine (IJMCM)}, issn = {2251-9637}, eissn = {2251-9645}, year = {2015} } @article{ author = {Fardaei, Majid and Sarrafzadeh, Shaghayegh and Ghafouri-Fard, Soudeh and Miryounesi, Mohamm}, title = {Autosomal Recessive Nonsyndromic Hearing Loss: A Case Report with a Mutation in TRIOBP Gene}, abstract ={Hearing loss (HL) is the most common sensory defect. Various genetic as well as environmental factors have been shown to contribute in it. More than 100 loci have been recognized to cause autosomal recessive nonsyndromic hearing loss (ARNSHL). Here, we report a 6-year old female patient with bilateral pre-lingual HL in whom a mutation has been identified in TRIOBP gene (c.6362C>T, S2121L). In silico analysis has shown that this variant is possibly pathogenic. Although several mutations have been detected in this gene in various populations, this is the first report identifying TRIOBP mutation in Iranian population. Consequently, the results of the present study may be of importance in genetic counseling.}, Keywords = {Hearing loss, TRIOBP, mutation}, volume = {4}, Number = {4}, pages = {245-247}, publisher = {Babol University of Medical Sciences}, url = {http://ijmcmed.org/article-1-398-en.html}, eprint = {http://ijmcmed.org/article-1-398-en.pdf}, journal = {International Journal of Molecular and Cellular Medicine (IJMCM)}, issn = {2251-9637}, eissn = {2251-9645}, year = {2015} }