en Genetics & Disease Genetics & Disease Original Article Original Article <p dir="ltr" style="text-align: justify;">Considering that MAPK (mitogen- activated protein kinase) signaling pathway has an important role in the progression of inflammatory cytokine secretion in type 2 diabetes mellitus (T2DM), we have recently investigated the reported genetic polymorphism from genome wide association study in <em>MAP₃K₁</em> (mitogen-activated protein kinase kinase kinase 1) in diabetes as an important member of MAPK signaling. This study aimed to investigate the possible association of rs10461617 at the upstream of <em>MAP₃K₁</em> gene in an Iranian case-control study with the risk of T2DM. The study population was comprised of 342 unrelated Iranian individuals including 177 patients with T2DM and 165 unrelated healthy control subjects. Genotyping was performed using PCR-RFLP and confirmed with sequencing. In a logistic regression analysis, the rs10461617A allele was associated with a significantly higher risk of T2DM assuming the log- additive model (OR: 1.44, 95% CI: 1.01-2.05, P = 0.039). In conclusion, we provided the first evidence for the association of rs10461617 at the upstream of <em>MAP₃K₁</em> with the risk of T2DM in an Iranian population.</p> Type 2 diabetes mellitus, genome- wide association study, MAP3K1, cytokines 134 140 http://ijmcmed.org/browse.php?a_code=A-10-822-1&slc_lang=en&sid=1 Shahram Torkamandi shahram.torkamandi@gmail.com 10031947532846007733 10031947532846007733 No Department of Medical Genetics, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran. Milad Bastami 10031947532846007734 10031947532846007734 No Department of Medical Genetics, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran. Hamid Ghaedi 10031947532846007735 10031947532846007735 No Department of Medical Genetics, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran. Fateme Moghadam 10031947532846007736 10031947532846007736 No Imam Hossein Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran. Reza Mirfakhraie 10031947532846007737 10031947532846007737 No Department of Medical Genetics, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran. Mir Davood Omrani davood_omrani@yahoo.co.uk 10031947532846007738 10031947532846007738 Yes Department of Medical Genetics, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.