International Journal of Molecular and Cellular Medicine
مجله بین المللی سلولی و مولکولی
Int J Mol Cell Med
Medical Sciences
http://ijmcmed.org
1
admin
2251-9637
2251-9645
10.22088/IJMCM.BUMS
en
jalali
1391
9
1
gregorian
2012
12
1
1
3
online
1
fulltext
en
Interaction of Memantine with Homocysteine on the Apoptosis in the Rat Hippocampus cells
Biomarkers (diagnosis & treatment)
Biomarkers (diagnosis & treatment)
Original Article
Original Article
It has been hypothesized that elevated plasma Homocysteine (Hcy) plays a role in the pathogenesis of Alzheimer’s disease (AD) and age-related cognitive decline. The mechanism of Hcy neurotoxicity in the brain is controversial as well Hcy is a ligand of NMDA receptor. Memantine, an uncompetitive antagonist of N-methyl-D-aspartate (NMDA) receptors approved for the treatment of moderate to severe Alzheimer's disease.
Hcy was injected 0.5 μmol/μl in the hippocampus of the rat brain and Memantine hydrochloride was injected 10mg/kg intraperitoneally 1 hour prior to Hcy injection. After five days, rats were killed and whole brain were taken out, fixed, and embedded in paraffin. The slices of the rat brain were prepared and immunohistochemical analysis was done to reveal the protein expression of Bax, Bcl-2, and the activation of Caspase 3 in the rat hippocampus layers. Results showed significant increase of Bax and Caspase-3 immunoreactivity in hippocampus of rat brain in Hcy group. Also an increase in Bax/Bcl-2 ratio in rat hippocampus cells .Memantine pretreatment could not change the levels of Bax, Bcl-2, Caspase-3 significantly in rat’s hippocampus cells.
These findings suggest that Memantine could not antagonize Hcy – induced Apoptosis. Hcy may induce apoptosis via the other oxidative stress mechanism in the rat brain. potential. It may therefore be interesting that he barberry fruit extracts has the unique capacity to quench free radicals.
Homocysteine, apoptosis. memantine, alzheimer’s disease, hippocampus, NMDA receptor, oxidative stress
145
152
http://ijmcmed.org/browse.php?a_code=A-10-79-1&slc_lang=en&sid=1
Amin
Ataie
ataieamin@yahoo.com
10031947532846002293
10031947532846002293
Yes
Cellular and Molecular Biology Research Center, Babol University of Medical Science, Babol, Iran.
Ramin
Ataee
ramin_ataee@yahoo.com
10031947532846002294
10031947532846002294
No
Pharmacy faculty, mazandaran university of medical science, Sari, Iran.
Mohmmad
Shadifar
shadan220@yahoo.com
10031947532846002295
10031947532846002295
No
Pastor Institute of Iran, Tehran, Iran.
Sima
Shahabi
sima.shabi@ymail.com
10031947532846002296
10031947532846002296
No
Cellular and Molecular Biology Research Center, Babol University of Medical Science, Babol, Iran.
Seyed Mohsen
Aghajanpour Mir
mohsenaghajanpour@gmail.com
10031947532846002297
10031947532846002297
No
Cellular and Molecular Biology Research Center, Babol University of Medical Science, Babol, Iran.
Younes
Hosseinpour
younes_h@yahoo.com
10031947532846002298
10031947532846002298
No
Pastor Institute of Iran, Tehran, Iran.