International Journal of Molecular and Cellular Medicine
مجله بین المللی سلولی و مولکولی
Int J Mol Cell Med
Medical Sciences
http://ijmcmed.org
1
admin
2251-9637
2251-9645
10.22088/IJMCM.BUMS
en
jalali
1394
7
1
gregorian
2015
10
1
4
4
online
1
fulltext
en
Association between Helicobacter pylori cagA, babA2 Virulence Factors and Gastric Mucosal Interleukin-33 mRNA Expression and Clinical Outcomes in Dyspeptic Patients
Infectious disease (Molecular and Cellular aspects)
Infectious disease (Molecular and Cellular aspects)
Original Article
Original Article
<p dir="ltr"><em>Helicobacter pylori</em> (<em>H. pylori</em>) infection has been reported in more than half of the world human population. It is associated with gastric inflammation and noticeable infiltration of the immune cells to the stomach mucosa by several cytokines secretion. IL-1&beta, IL-18 have been shown to contribute to <em>H. pylori</em> induced gastritis, but the details of inflammation and association of virulence factors remain unclear. IL-1 cytokine family has a new additional cytokine, Interleukin-33 (IL-33), which is contemplated to have an important role for host defense against microorganisms. <em>H. pylori</em> virulence factors important in gastritis risk are the cag pathogenicity island (cag-PAI) and babA. This study evaluated IL-33 mucosal mRNA expression levels in infected and uninfected patients and its relationship with bacterial virulence factors cagA, babA<sub>2</sub> and type of gastritis. Total RNA was extracted from gastric biopsies of 79 <em>H. pylori</em>-infected patients and 51 <em>H. pylori</em>-negative patients. Mucosal IL-33 mRNA expression levels in gastric biopsies were assessed using real-time PCR. Existence of virulence factors were detected by PCR. IL-33 mRNA expression was significantly higher in biopsies of <em>H. pylori</em>-infected patients compared to <em>H. pylori</em>-uninfected patients (P<0.0001). Also there was a direct relationship between virulence factor bab-A<sub>2</sub> and enhancement in IL-33 mRNA expression. Furthermore, IL-33 mRNA expression level was significantly lower in chronic gastritis patients compared with patients with active gastritis (P<0.001). IL-33 may play a crucial role in the inflammatory response and induction of the chronic gastritis and severity of inflammatory changes in the gastric mucosa.</p>
Helicobacter pylori, gastritis, interleukin-33, virulence factor
227
234
http://ijmcmed.org/browse.php?a_code=A-10-257-2&slc_lang=en&sid=1
Heshmat
Shahi
heshmat.shahi@gmail.com
10031947532846006027
10031947532846006027
No
Cellular and Molecular Research Center, Shahrekord University of Medical Sciences, Shahrekord, Iran.
Somayeh
Reiisi
s.reiisi@yahoo.com
10031947532846006028
10031947532846006028
No
Department of Genetic, Faculty of basic science, Shahrekoed University, Shahrekord, Iran
Rasol
Bahreini
abr_abr021@yahoo.com
10031947532846006029
10031947532846006029
No
Department of Internal Medicine, Shahrekord University of Medical Sciences, Shahrekord, Iran
Nader
Bagheri
naderbagheri@gmail.com
10031947532846006030
10031947532846006030
No
Cellular and Molecular Research Center, Shahrekord University of Medical Sciences, Shahrekord, Iran.
Loghman
Salimzadeh
abrabr021@yahoo.com
10031947532846006031
10031947532846006031
No
Cellular and Molecular Research Center, Shahrekord University of Medical Sciences, Shahrekord, Iran.
Hedayatollah
Shirzad
shirzadeh@yahoo.com
10031947532846006032
10031947532846006032
Yes
Cellular and Molecular Research Center, Shahrekord University of Medical Sciences, Shahrekord, Iran.