en Infectious disease (Molecular and Cellular aspects) Infectious disease (Molecular and Cellular aspects) Original Article Original Article <p style="text-align: justify;"><span style="font-family:Tahoma;"><span style="font-size:11pt"><span style="line-height:14.0pt"><span lang="EN-GB" style="font-size:10.0pt">Although biofilms on endotracheal tube (ET) surfaces represent a major clinical challenge, studies addressing the effect of lytic bacteriophages on these biofilms are relatively scarce. This study focused on examining the anti-biofilm capability of three specific phage against an XDR isolate of <i>Acinetobacter baumannii</i> in a 48-hour pre-formed biofilm on an ET surface. For this purpose, crystal violet staining, colony counting, and scanning electron microscopy (SEM) were employed. The results demonstrated a significant decrease in biofilm mass and bacterial count after 24 hours of exposure to the phage cocktail. SEM images confirmed a dramatic reduction in the biofilm. Based on these findings, phage therapy has the potential to reduce and disrupt biofilms on ET surfaces. </span></span></span></span></p> Bacteriophage, Biofilm, Acinetobacter baumannii, Endotracheal Tube 1059 1071 http://ijmcmed.org/browse.php?a_code=A-10-70-3&slc_lang=en&sid=1 Susan Khanjani khanjani1650@gmail.com 100319475328460036805 100319475328460036805 No Medical Microbiology Research Center, Qazvin University of Medical Sciences, Qazvin, Iran Farhad Nikkhahi farhadnikkhahi@gmail.com 100319475328460036806 100319475328460036806 No Medical Microbiology Research Center, Qazvin University of Medical Sciences, Qazvin, Iran Fatemeh Zeynali Kelishomi pzeynali1996@gmail.com 100319475328460036807 100319475328460036807 No Department of Microbiology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran Amir Javadi javadi_a@yahoo.com 100319475328460036808 100319475328460036808 No Qazvin University of Medical Sciences, Qazvin, Iran Sara Sadeghi Sarasadeghi@isu.eud 100319475328460036809 0009-0002-2271-7961 No Department of Biological Sciences, Idaho State University, Pocatello, Idaho, USA Behzad Bijani dr.bijani@gmail.com 100319475328460036810 100319475328460036810 No Medical Microbiology Research Center, Qazvin University of Medical Sciences, Qazvin, Iran Hamid Sadeghi h.sadeghi93@yahoo.com 100319475328460036811 100319475328460036811 No Medical Microbiology Research Center, Qazvin University of Medical Sciences, Qazvin, Iran Hediyeh Saghi Sarabi saghisarabi99@gmail.com 100319475328460036812 100319475328460036812 No Medical Microbiology Research Center, Qazvin University of Medical Sciences, Qazvin, Iran Seyed Mahmoud Amin Marashi parsmicrob@gmail.com 100319475328460036813 100319475328460036813 Yes Medical Microbiology Research Center, Qazvin University of Medical Sciences, Qazvin, Iran