en Histopathology Histopathology Original Article Original Article <div style="text-align: justify;"><span style="font-family:Tahoma;"><span style="font-size:11pt"><span style="line-height:107%"><span style="font-size:10.0pt"><span style="background:white"><span style="line-height:107%">Improving wound care products to facilitate effective skin repair is very important. Hydrogels are promising polymer-based dressings that enhance wound healing. Vitamin E can improve skin injuries by increasing antioxidant capacity. This study aimed to construct an innovative hydrogel from carboxymethyl cellulose (CMC) and gelatin (Gel) containing vitamin E to enhance wound healing.</span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:107%"><span style="font-size:10.0pt"><span style="background:white"><span style="line-height:107%">Five unique hydrogel formulations were constructed by combination of CMC-Gel and 25, 50, 100, and 200 &micro;L/mL of Vitamin E. Structural characteristics of hydrogels were assessed using scanning electron microscopy (SEM) and Fourier-transform infrared spectroscopy (FTIR). Biochemical properties of hydrogels, including swelling, weight loss, pH values, and blood compatibility, were evaluated by specific methods. The cytotoxicity effects of hydrogels on the NIH-3T3 fibroblasts were determined by MTT assay. The therapeutic potential of the hydrogels was investigated using a full-thickness wound model in Wistar rats. The constructed CMC-Gel-Vit E hydrogel had a porous structure characterized by interconnected voids measuring 73.15&plusmn;9.61 &micro;m, which is favorable for promoting cell migration. The cytotoxicity results showed no toxicity effects of hydrogels with and without VitE 100 &micro;L/mL, also a survival rate of over 120% in cells after 72 h. In vivo data showed the CMC-Gel-Vit E hydrogel (91.36&plusmn;8.23%) significantly increased the percentage of wound closure and re-epithelialization compared to the control group (68.31&plusmn;13.59%).</span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:107%"><span style="font-size:10.0pt"><span style="background:white"><span style="line-height:107%">The results highlight the considerable potential of the CMC-Gel-VitE hydrogel as a viable option for skin regeneration and wound healing. This hydrogel exhibits substantial promise for use in clinical and therapeutic interventions.</span></span></span></span></span></span><span style="font-size:11pt"><span style="line-height:107%"><span style="font-family:Calibri,sans-serif"><span style="font-size:10.0pt"><span style="background:white"><span style="line-height:107%"><span new="" roman="" style="font-family:" times=""><i></i></span></span></span></span></span></span></span><br> &nbsp;</div> Skin Regeneration, Hydrogel dressing, NIH 3T3 Cells, Tocopherols, Biopolymers 808 827 http://ijmcmed.org/browse.php?a_code=A-10-262-7&slc_lang=en&sid=1 Seyed-Reza Mousavi reza_mousavi91@yahoo.com 100319475328460035820 0009-0008-3361-5796 No Department of Clinical Biochemistry, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran Mojtaba Rashidi rashidi-mo@ajums.ac.ir 100319475328460035821 100319475328460035821 No Hyperlipidemia Research Center, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran. Khedri Azam khedri-a@ajums.ac.ir 100319475328460035822 100319475328460035822 No Department of Clinical Biochemistry, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran. Maryam Kouchak kouchak-m@ajums.ac.ir 100319475328460035823 100319475328460035823 No Department of Pharmaceutics, Faculty of Pharmacy, Nanotechnology Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran. Majid Salehi msalehi.te1392@gmail.com 100319475328460035824 100319475328460035824 No Regenerative Medicine Research Center, Shahroud University of Medical Sciences, Shahroud, Iran. Ghorban Mohammadzadeh mohammadzadeh@ajums.ac.ir 100319475328460035825 100319475328460035825 Yes Hyperlipidemia Research Center, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.