en Cancer Cancer Original Article Original Article <div style="text-align: justify;"><span style="font-size:12px;"><span style="font-family:Tahoma;"><span style="line-height:107%"><span style="color:black">Colorectal cancer (CRC), which is among the most prevalent cancers worldwide, is caused by environmental and genetic factors. It has been shown that the <i>p53</i> gene is associated with CRC pathogenesis; moreover, Zyxin (ZYX) may play a role in <i>p53</i> level and activity. Therefore, the present research aimed to investigate the levels of P53 and <i>ZYX</i> genes and proteins in CRC tumor samples. Cancerous tissues (n=31) and matched non-cancerous tissues (n=31) were randomly obtained from 31 patients with CRC. Total RNA was extracted using RNXplus, and gene expressions were assessed by Real-time PCR. Furthermore, the Western blot technique was used to investigate the expression of ZYX and P53 proteins. The obtained results revealed that the expression of<i> ZYX</i> and <i>p53</i> genes in cancerous tissues showed no significant difference compared to matched non-cancerous tissues. On the other hand, measuring protein expression using the Western blotting technique indicated that the ZYX (P=0.0081) and <i>p53</i> (P=0.0065) expression in tumor tissues significantly decreased compared to those in matched non-cancerous tissues. Correlation analysis indicated a significant correlation between ZYX and P53 proteins (r=0.746, P=0.013). Based on our findings, ZYX might have a suppressive function in CRC and is associated with P53.</span></span></span></span></div> Colorectal neoplasms, Tumor suppressor protein p53, Zyxin 462 471 http://ijmcmed.org/browse.php?a_code=A-10-2969-2&slc_lang=en&sid=1 Hossein Mohammadi hoseinmohammadi9473@gmail.com 100319475328460033566 100319475328460033566 No Department of Clinical Biochemistry, Kermanshah University of Medical Sciences, Kermanshah, Iran. Ebrahim Shakiba m.shakiba_d91@yahoo.com 100319475328460033567 100319475328460033567 No Department of Clinical Biochemistry, Kermanshah University of Medical Sciences, Kermanshah, Iran. Rezvan Rostampour rostampour.rezvan@yahoo.com 100319475328460033568 100319475328460033568 No Clinical Research Development Center, Imam Reza Hospital, Kermanshah University of Medical Sciences, Kermanshah, Iran. Kiana Bahremand kianabahremand@yahoo.com 100319475328460033569 0009-0003-0314-961X No Nano Drug Delivery Research Center, Health Technology Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran. Mohammad Taghi Goodarzi mtgoodarzi@yahoo.com 100319475328460033570 100319475328460033570 No Department of Biochemistry, Shahrood Branch, Islamic Azad University, Shahrood, Iran. Homayoon Bashiri hbashirimd@gmail.com 100319475328460033571 100319475328460033571 No Department of Internal Medicine, Infectious Disease Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran. Khadijeh Najafi Ghobadi kh.najafi420@yahoo.com 100319475328460033572 100319475328460033572 No Department of Biostatistics, Faculty of Health, Ilam University of Medical Sciences, Ilam, Iran. Soheila Asadi sohila.asadi75@yahoo.com 100319475328460033573 100319475328460033573 Yes Fertility and Infertility Research Center, Health Technology Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran