en Infectious disease (Molecular and Cellular aspects) Infectious disease (Molecular and Cellular aspects) Original Article Original Article <span style="font-family:Tahoma;"><span style="font-size:12px;"><span style="unicode-bidi:embed"><span style="line-height:107%">Rosemary is an aromatic plant with ancient and modern applications as a spice and herbal remedy. Due to the strong antioxidant potential of rosemary, the present study investigated the anti-proliferative and pro-apoptotic characteristics of rosemary on luminal A and triple-negative breast cancer cells. The effect of rosemary extract on the WNT10B and &beta;-Catenin genes was also evaluated. The WNT10B and &beta;-Catenin expression were measured by real-time PCR.&nbsp; The outcomes of the MTT assay and AnnexinV/PI flow cytometry assay showed that exposure of MCF-7 and MDA-MB-231 cells to rosemary reduced cell viability in a dose-time-dependent routine and promoted apoptosis in breast cancer cells. It was revealed that the extract could exert cytotoxic and apoptotic effects by downregulation of WNT10B and &beta;-Catenin. Our results suggest rosemary as a promising complementary herbal medicine for breast cancers, without the adverse effects of chemotherapy drugs<span dir="RTL" lang="FA">.</span></span></span></span></span> Rosmarinus officinalis, luminal A, triple-negative breast cancer, anti-proliferative, pro-apoptotic, Wnt/ β-catenin 198 209 http://ijmcmed.org/browse.php?a_code=A-10-8023-1&slc_lang=en&sid=1 Kamran Eghbalpour kamraneghbalpour.2017@gmail.com 100319475328460032781 0009-0009-3802-778X No Department of Biology, Shahr-e-Qods Branch, Islamic Azad University, Tehran, Iran. Nahid Eghbalpour Nahideghbalpour300@gmail.com 100319475328460032782 0009-0007-8874-4539 No Department of Biology, Shahr-e-Qods Branch, Islamic Azad University, Tehran, Iran. Saideh Khademi Skhademi1977@gmial.com 100319475328460032783 0009-0000-0974-897X No Department of Biology, Shahr-e-Qods Branch, Islamic Azad University, Tehran, Iran. Laleh Arzi Laleh.arzi@ut.ac.ir 100319475328460032784 100319475328460032784 Yes Department of Microbiology, Shahr-e-Qods Branch, Islamic Azad University, Tehran, Iran.