en Molecular & Cellular Immunology Molecular & Cellular Immunology Original Article Original Article <span style="font-family:Tahoma;"><span style="font-size:12px;"><span style="unicode-bidi:embed"><span style="line-height:107%">One of the important stimulating molecules for the function of T lymphocytes is tumor necrosis factor receptor OX40 (CD134), activated by its cognate ligand OX40L (CD134L, CD252). OX40L interactions have been proposed as a potential therapeutic target for treating infectious and non-infectious diseases. The main purpose of this study was to determine the potency of two novel resins MBI and MEP for the purification of OX40L-IgG fusion protein and the biological activities of this OX40L-IgG fusion protein. The biological activity of the OX40L-IgG purified by these resins compared with protein A sepharose resin. Mice treated with the same doses of the OX40L purified by the three resins showed a significant delay in tumor growth compared to the controls injected with PBS. Mice treated with the OX40L purified by MBI resin showed a significant delay in tumor cell (CT26) growth compared with mice injected with OX40L purified by other resins<span dir="RTL" lang="FA">.</span></span></span></span></span> OX40L-IgG, purification, MBI, MEP 335 349 http://ijmcmed.org/browse.php?a_code=A-10-6800-1&slc_lang=en&sid=1 Hossein Rezvan h.rezvan@basu.ac.ir 100319475328460033906 100319475328460033906 Yes Department of Biology, Central Tehran Branch, Islamic Azad University, Tehran, Iran. Selman A Ali selman.ali@ntu.ac.uk 100319475328460033907 100319475328460033907 No School of Science, Nottingham Trent University, Clifton Lane, Clifton, Nottingham, NG11 8NS, UK. Sahar Hamoon Navard s.hamoonnavard@basu.ac.ir 100319475328460033908 100319475328460033908 No Department of Pathobiology, Faculty of Veterinary Sciences, Bu-Ali Sina University, Hamedan, Iran. Robert Rees robert.rees@ntu.ac.uk 100319475328460033909 100319475328460033909 No Department of Life Sciences, Nottingham Trent University, Clifton Lane, Nottingham NG11 8NS, UK.