International Journal of Molecular and Cellular Medicine
مجله بین المللی سلولی و مولکولی
Int J Mol Cell Med
Medical Sciences
http://ijmcmed.org
1
admin
2251-9637
2251-9645
10.22088/IJMCM.BUMS
en
jalali
1401
8
1
gregorian
2022
11
1
11
2
online
1
fulltext
en
hsa-miR-508-5p as a New Potential Player in Intervertebral Disc Degeneration
Other
Other
Original Article
Original Article
<span style="font-family:Tahoma;"><span style="font-size:12px;"><span style="line-height:200%"><span style="background:white"><span style="line-height:200%">Intervertebral disc degeneration (IDD) is widely known as the principal cause of low back pain, diminishing patients’ quality of life and imposing a huge economic burden on healthcare systems worldwide. However, the underlying mechanisms of IDD remain to be determined. This study aimed to scrutinize data sets via bioinformatics to identify microRNAs (miRNAs)/genes and pathways associated with IDD. The array profiling of patients with IDD and individuals without IDD was acquired from the Gene Expression Omnibus (GEO) database (viz., GSE19943, GSE63492, and GSE34095). The expression profiles of miRNAs and genes with differential patterns were analyzed using GEO2R. The target genes of the chosen miRNA were then examined, and <i>in silico </i>functional analyses were performed on the signaling pathways and biological processes of the differentially expressed genes. Three human miRNAs were up and downregulated in IDD patients in the examined data sets. Among them, hsa-miR-508-5p had a significant differential expression in the IDD group, and <i>SEC11A, IPO5, FN1</i>, and <i>MRPS10</i>, as the targets of hsa-miR-508-5p, were upregulated in the IDD group. Furthermore, extracellular matrix-receptor interactions, focal adhesion, and actin cytoskeleton regulation were important pathways involved in IDD. Our analysis identified hsa-miR-508-5p as a novel miRNA involved in IDD pathogenies. Our findings not only further confirm the significant role of miRNAs in IDD pathogenesis but also extend the spectrum of the miRNAs and genes involved in IDD. Still, further experimental investigations are needed to confirm our findings.</span></span></span></span></span>
Intervertebral disc degeneration, hsa-miR-508-5p, Noncoding RNA regulators, Low back pain
137
149
http://ijmcmed.org/browse.php?a_code=A-10-5096-1&slc_lang=en&sid=1
Akram
Gholipour
akram.gholipour199069@gmail.com
100319475328460026158
100319475328460026158
No
Cardiogenetic Research Center, Rajaie Cardiovascular Medical and Research Center, Iran University of Medical Sciences, Tehran, Iran.
Mahshid
Malakootian
Mahshid.malakootian@gmail.com
100319475328460026159
100319475328460026159
No
Cardiogenetic Research Center, Rajaie Cardiovascular Medical and Research Center, Iran University of Medical Sciences, Tehran, Iran.
Maziar
Oveisee
Maziar.oveisee@gmail.com
100319475328460026160
100319475328460026160
Yes
School of Medicine, Bam University of Medical Sciences, Bam, Kerman, Iran.