International Journal of Molecular and Cellular Medicine
مجله بین المللی سلولی و مولکولی
Int J Mol Cell Med
Medical Sciences
http://ijmcmed.org
1
admin
2251-9637
2251-9645
10.22088/IJMCM.BUMS
en
jalali
1400
2
1
gregorian
2021
5
1
10
2
online
1
fulltext
en
Mesenchymal Stem Cells cause Telomere Length Reduction of Molt-4 Cells via Caspase-3, BAD and P53 Apoptotic Pathway
Stem Cell
Stem Cell
Original Article
Original Article
Mesenchymal stem cells (MSCs) as undifferentiated cells are specially considered in cell-based cancer therapy due to unique features such as multi-potency, pluripotency, and self-renewal. A multitude of cytokines secreted from MSCs are known to give such multifunctional attributes, but details of their role are yet to be unknown. In the present study, MSCs were cultured, characterized and co-cultured with Molt-4 cells as acute lymphoblastic leukemia cell line in a trans-well plate. Then, cultured Molt-4 alone and Molt-4 co-cultured with MSCs (10:1) were collected on day 7 and subjected to real time-PCR and Western blotting for gene and protein expression assessment, respectively. Ki-67/caspase-3 as well as telomere length were investigated by flow cytometry and real time-PCR, respectively. The results showed that MSCs caused significant decrease in telomere length as well as <em>hTERT</em> gene expression of Molt-4 cells. Also, gene and protein expression of BAD and P53 were significantly increased. Furthermore, the flow cytometry analysis indicated the decrease and increase of the Ki-67 and caspaspase-3 expression, respectively. It was concluded that MSCs co-cultured with Molt-4 cells could be involved in the promotion of Molt-4 cell apoptosis via caspase-3, BAD, and P53 expression. In addition, the decrease of telomere length is another effect of MSCs on Molt-4 leukemic cells.
Mesenchymal stem cells, telomere length, hTERT, BAD, P53, caspase-3, apoptotic pathway
113
122
http://ijmcmed.org/browse.php?a_code=A-10-3464-1&slc_lang=en&sid=1
Hamid Reza
Heidari
heidarih@tbzmed.ac.ir
100319475328460021193
100319475328460021193
No
Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
Ezzatollah
Fathi
ez.fathi@tabrizu.ac.ir
100319475328460021194
100319475328460021194
No
Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran.
Soheila
Montazersaheb
smontazersaheb@gmail.com
100319475328460021195
100319475328460021195
No
Molecular Medicine Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
Ayoub
Mamandi
ayob.mamandi@yahoo.com
100319475328460021196
100319475328460021196
No
Student Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran.
Raheleh
Farahzadi
farahzadir@tbzmed.ac.ir
100319475328460021197
100319475328460021197
Yes
Hematology and Oncology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
Soran
Zalavi
soranzalavi@yahoo.com
100319475328460021198
100319475328460021198
No
Student Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran.
Hojjatollah
Nozad Charoudeh
nozadch@tbzmed.ac.ir
100319475328460021199
100319475328460021199
No
Department of Anatomical Sciences, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran.