International Journal of Molecular and Cellular Medicine
مجله بین المللی سلولی و مولکولی
Int J Mol Cell Med
Medical Sciences
http://ijmcmed.org
1
admin
2251-9637
2251-9645
10.22088/IJMCM.BUMS
en
jalali
1392
11
1
gregorian
2014
2
1
3
2
online
1
fulltext
en
Tissue Culture Study of the Medicinal Plant Leek (Allium ampeloprasum L.)
Other
Other
Original Article
Original Article
Persian shallot, also called leek (Allium ampeloprasum), is a monocotyledon plant of the lily family (Liliaceae). It belongs to the genus Allium, has a characteristic taste and morphological features, and is considered as one of the oniony vegetables. This research was conducted with the purpose of obtaining optimal conditions for tissue culture of Persian shallot and of comparing calli and leaf tissues of this plant with respect to in vivo presence of ethereal oils. In this study, the auxin hormone 2, 4–D at three levels (0.5, 0.1, and zero), the hormone BAP at three levels (0.5, 0.1, and zero), and Kin at two levels (zero, and 0.5) were used in the format of a randomized complete block design in three replications. Results obtained showed that the best culture for callus formation for explants – leaf samples and the best culture for callus formation in explants – seed samples were the MS cultures with the hormonal compositions (0.5 mg of 2, 4–D, 0.1 mg of BAP) and (0.5 mg of Kin and 0.1 mg of 2, 4–D). Identification of the chemical composition of the essential oils of the various species of this plant was carried out by using an essential oil analysis GC mass. Twenty one compounds were observed in the column obtained from the GC mass, seven of which (constituting about 51.5% of the total amount of compounds present in the essential oils) were identified.
Leek, Allium ampeloprasum, tissue culture, chromatography, essential oil
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125
http://ijmcmed.org/browse.php?a_code=A-10-125-1&slc_lang=en&sid=1
Mohammad Bagher
Monemi
10031947532846002993
10031947532846002993
No
Department of Agriculture, Payame Noor University, Iran.
Kamal
Kazemitabar
10031947532846002994
10031947532846002994
No
Sari Agricultural Science and Natural Resource University, Iran.
Gholamreza Bakhshee
Khaniki
10031947532846002995
10031947532846002995
No
Department of Agriculture, Payame Noor University, Iran.
Esmaeil
Yasari
10031947532846002996
10031947532846002996
No
Department of Agriculture, Payame Noor University, Iran.
Firouzeh
Sohrevardi
10031947532846002997
10031947532846002997
No
Gabit and Agricultural Biotecnology Institute of Tabarestan, Sari Agricultural Sciences and and Natural Resources University, Iran.
Roghayeh
Pourbagher
r_pourbagher17@yahoo.com
10031947532846002998
10031947532846002998
Yes
Cellular and Molecular Biology Research Center (CMBRC) Babol University of Medical Sciences, Babol, Iran.