International Journal of Molecular and Cellular Medicine
مجله بین المللی سلولی و مولکولی
Int J Mol Cell Med
Medical Sciences
http://ijmcmed.org
1
admin
2251-9637
2251-9645
10.22088/IJMCM.BUMS
en
jalali
1399
5
1
gregorian
2020
8
1
9
3
online
1
fulltext
en
Evaluation of Anti-inflammatory and Antioxidant Profile of Oleuropein in Experimentally Induced Ulcerative Colitis
Biomarkers (diagnosis & treatment)
Biomarkers (diagnosis & treatment)
Original Article
Original Article
<p>Oleuropein is one of the main phenolic secoiridoid of the olive leaf extract, which is known for its antioxidant and anti-inflammatory effects. The main objective of the present study was to investigate the effectiveness of oleuropein in the ulcerative colitis treatment. <a name="_gjdgxs"></a>An experimental study was designed on rats, which were divided into three groups, group 1 (normal control), group 2 (induced for ulcerative colitis and untreated), and group 3 (induced for ulcerative colitis and treated with oleuropein). Colonic tissue samples were collected from all studied groups and the oxidative stress and antioxidant activity were assessed by evaluating malondialdehyde (MDA), superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPX), myeloperoxidase (MPO), and nitric oxide (NO) levels. The expression levels of pro-inflammatory cytokines such as <em>IL-1β</em>, <em>TNF-α</em>, <em>IL-10</em>, <em>COX-2</em>, <em>iNOS</em>, <em>TGF-β1</em>, <em>MCP-1</em>, and <em>NF-κB</em>, the pro-apoptotic gene <em>Bax</em>, and the anti-apoptotic gene <em>Bcl2</em> were assessed in colon tissues to evaluate the effectiveness of oleuropein treatment. Oleuropein was effective on reducing the mortality rate and disease activity index. Oleuropein caused a significant reduction in colon MDA, MPO, and NO levels and a significant elevation in SOD, CAT, and GPX levels and induced the down regulation of analyzed proinflammatory cytokines. Also, downregulation of <em>Bax</em> and upregulation of <em>Bcl2</em> were observed as a result of oleuropein treatment in comparison with untreated acetic acid induced ulcerative colitis group. Oleuropein showed intestinal anti-inflammatory, antioxidant, and anti-apoptotic effects in ulcerative colitis experimental model.</p>
Ulcerative colitis, oleuropein, anti-inflammatory, antioxidant, Bax, Bcl2
224
233
http://ijmcmed.org/browse.php?a_code=A-10-2643-1&slc_lang=en&sid=1
Muhammed Hassan
Motawea
100319475328460018963
100319475328460018963
No
Biochemistry Department, Faculty of Science, Tanta University, Egypt.
Hussein Abd
Elmaksoud Ali
100319475328460018964
100319475328460018964
No
Biochemistry Department, Benha University, Egypt.
Mohamed Gamal
Elharrif
al_harrif@yahoo.com
100319475328460018965
100319475328460018965
Yes
Department of Basic Medical Sciences, Shaqra University, KSA.
Afaf Abd
Elmajeed Desoky
100319475328460018966
100319475328460018966
No
Biochemistry Department, Benha University, Egypt.
Asmaa
Ibrahimi
100319475328460018967
100319475328460018967
No
Genetic Engineering and Biotechnology Research Institute, University of Sadat City, Sadat, Egypt.