en Bioinformatic Bioinformatic Original Article Original Article <div style="text-align: justify;">Tumor lysis syndrome is a life-threatening condition for humans due to the lack of urate oxidase. In this study, several variants of PASylateduricasefrom the <em>Aspergillus flavus</em> species were analyzed computationally to find the appropriate fusions to solve short half-life and stability concerns. The <em>Ab initio</em> method was performed using Rosetta software to structurally characterize the PAS sequences. The 3D structures of fusions were predicted for fused C- or N-terminally PAS sequences in different lengths to the uricase. The refinement and energy minimization steps revealed that physicochemical and conformational properties of fusions improved while the structures possessed prolonged PAS sequences. Molecular docking results showed that the highest binding affinity to uric acid belonged to uricase-PAS1-100 by the formation of six hydrogens and four non-hydrogen bonds. Altogether, the results indicated that the PASylationprocess would be promising upon the production of urate oxidase with improved solubility and stability.</div> Tumor lysis syndrome, Aspergillus flavus, uricase, molecular dynamic, PASylation, plasma half-life 90 106 http://ijmcmed.org/browse.php?a_code=A-10-2568-1&slc_lang=en&sid=1 Abbas Najjari abbas.najjari@gmail.com 100319475328460018909 100319475328460018909 No Enzyme Technology Laboratory, Department of Biochemistry,Genetic and Metabolism Research Group, Pasteur Institute of Iran,Tehran, Iran. Hamzeh Rahimi rahimi.h1981@gmail.com 100319475328460018910 100319475328460018910 No Molecular Medicine Department, Pasteur Institute of Iran, Tehran, Iran. Seyed Ali Nojoumi ali.nojoumi@gmail.com 100319475328460018911 100319475328460018911 No Microbiology Research Center, Pasteur Institute of Iran, Tehran, Iran. Eskandar Omidinia eirani.pasteur1299@gmail.com 100319475328460018912 100319475328460018912 Yes Enzyme Technology Laboratory, Department of Biochemistry,Genetic and Metabolism Research Group, Pasteur Institute of Iran,Tehran, Iran.