International Journal of Molecular and Cellular Medicine
مجله بین المللی سلولی و مولکولی
Int J Mol Cell Med
Medical Sciences
http://ijmcmed.org
1
admin
2251-9637
2251-9645
10.22088/IJMCM.BUMS
en
jalali
1399
3
1
gregorian
2020
6
1
9
2
online
1
fulltext
en
Evaluation of miRNAs Related with Nuclear Factor Kappa B Pathway in Lipopolysaccharide Induced Acute Respiratory Distress Syndrome
Infectious disease (Molecular and Cellular aspects)
Infectious disease (Molecular and Cellular aspects)
Original Article
Original Article
This study aimed to determine the expression of nuclear factor kappa B (NF-κB) pathway related miRNAs in experimental acute respiratory distress syndrome (ARDS) induced by lipopolysaccharide (LPS) in rats, and to elucidate the underlying molecular mechanism. Twenty four sprague dawley rats were randomly divided into two groups; LPS (n = 12) and control (n = 12). Experimental ARDS was induced by intraperitoneal injection of <em>E. coli</em> LPS in LPS group. Intraperitoneal saline was administered in control group. Serum and lung samples were collected from both groups. Immunohistochemistry staining was performed for interleukin 1β (IL-1β), tumor necrosis factor α (TNF-α), CD 68, and caspase-3 in lung samples. Intensity of staining was scored as strong, moderate, weak, and no for evaluation of IL-1β and TNF-α. In addition, caspase-3 and CD 68 positive stained cells were counted in sections. Expressions of 9 miRNAs were determined by quantitative real-time PCR in serum samples. IL-1β and TNF-α staining scores were significantly higher in the LPS group in comparison with the control group (P = 0.04 and P = 0.02, respectively). In addition, caspase-3 and CD 68 positive stained cells were significantly higher in the LPS group (P = 0.02). Expressions of seven miRNAs were significantly changed in the LPS group in comparison with the control group. While six miRNAs (miR-7a-5p, miR-7b, miR-9a-5p, miR-21-5p, miR-29a-3p and miR-138-5p) were up regulated, only miR-124-3p was down-regulated. This study suggests that these miRNAs may have a role in the pathogenesis of ARDS related to NF-κB. However, this relationship needs to be examined in new studies by evaluation of pathways and target genes.
Acute respiratory distress syndrome, ARDS, microRNA, nuclear factor kappa B
130
139
http://ijmcmed.org/browse.php?a_code=A-10-2208-1&slc_lang=en&sid=1
Mustafa
Azizoglu
dryalamaoglu@hotmail.com
100319475328460017850
100319475328460017850
No
Mersin University, Faculty of Medicine, Department of Anesthesiology and Reanimation, Mersin, Turkey.
Lokman
Ayaz
lokmanayaz@yahoo.com
100319475328460017851
100319475328460017851
Yes
Trakya University, Faculty of Pharmacy, Department of Biochemistry, Edirne, Turkey.
Gülsen
Bayrak
gulsenbayrak@mersin.edu.tr
100319475328460017852
100319475328460017852
No
Mersin University, Faculty of Medicine, Department of Histology & Embriyology, Mersin, Turkey.
Banu
Çoskun Yılmaz
bcoskun@mersin.edu.tr
100319475328460017853
100319475328460017853
No
Mersin University, Faculty of Medicine, Department of Histology & Embriyology, Mersin, Turkey.
Handan
Birbiçer
handanbirbicer@mersin.edu.tr
100319475328460017854
100319475328460017854
No
Mersin University, Faculty of Medicine, Department of Anesthesiology and Reanimation, Mersin, Turkey.
Nurcan
Doruk
nurcandoruk@mersin.edu.tr
100319475328460017855
100319475328460017855
No
Mersin University, Faculty of Medicine, Department of Anesthesiology and Reanimation, Mersin, Turkey.