International Journal of Molecular and Cellular Medicine
مجله بین المللی سلولی و مولکولی
Int J Mol Cell Med
Medical Sciences
http://ijmcmed.org
1
admin
2251-9637
2251-9645
10.22088/IJMCM.BUMS
en
jalali
1398
8
1
gregorian
2019
11
1
8
4
online
1
fulltext
en
Diagnostic Value of Plasma Long Non-coding RNA HOTTIP as a Non-invasive Biomarker for Colorectal Cancer ( A Case- Control Study)
Biomarkers (diagnosis & treatment)
Biomarkers (diagnosis & treatment)
Original Article
Original Article
<div style="text-align: justify;">Long non-coding RNAs (lncRNAs) associated with various cancers, including colorectal cancer (CRC), could be collected from body fluids easily. Our aims were to determine the expression level of <em>HOTTIP</em> lncRNA in plasma samples of healthy individuals and CRC patients as well as their relationship with clinico-pathological characteristics of patients. First, total RNA was extracted from the plasma samples of 100 subjects including 50 patients and 50 age and sex matched healthy persons. Then, gene expression was measured using real-time PCR technique. The sensitivity and specificity of <em>HOTTIP</em> dysregulation in CRC and healthy individual’s plasma was measured by receiver operating characteristic (ROC) analysis. As compared with healthy controls, <em>HOTTIP</em> lncRNA was over expressed in a statistically significant manner in plasma samples of patients (P = 0.001). Significant relationship between <em>HOTTIP</em> expression and positive family history of CRC was observed, too (P = 0.04). The ROC curve analysis showed an AUC value of 0.775, a specificity of 82%, a sensitivity of 76%, with a cut off value equal to 2.40 (P = 0.001). <em>HOTTIP</em> transcript can be proposed as a new biomarker for early diagnosis due to the increased expression in plasma samples of patients with CRC and the relatively high sensitivity and specificity.</div>
Biomarker, colorectal neoplasms, long non-coding RNAs, lncRNA HOTTIP
240
246
http://ijmcmed.org/browse.php?a_code=A-10-314-5&slc_lang=en&sid=1
Soheila
Ali akbar- Esfahani
soheilaaa.esfahany@gmail.com
100319475328460016627
100319475328460016627
No
Department of Molecular Medicine & Genetics, Hamadan University of Medical Sciences, Hamadan, Iran.
Morteza
Karimipoor
mortezakarimi@yahoo.com
100319475328460016628
100319475328460016628
No
Molecular Medicine Department, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran.
Fatemeh
Bahreini
f.bahreini@yahoo.com
100319475328460016629
100319475328460016629
No
Department of Molecular Medicine & Genetics, Hamadan University of Medical Sciences, Hamadan, Iran.
Ali Reza
Soltanian
arsoltanian@yahoo.com
100319475328460016630
100319475328460016630
No
Modelling of Non communicable Diseases Research Center, School of Public Health, Hamadan University of Medical Sciences, Hamadan, Iran.
Najme
Aletaha
dr.aletaha@gmail.com
100319475328460016631
100319475328460016631
No
Department of Gastroenterology, Imam Khomeini Hospital, Tehran University of Medical Science, Tehran, Iran.
Ali
Mahdavinezhad
alimahdavin@gmail.com
100319475328460016632
100319475328460016632
Yes
Research center for Molecular Medicine, Department of Molecular Medicine and Genetics, Hamadan University of Medical Sciences, Hamadan, Iran.