%0 Journal Article %A Mercaldi Pastrelo, Maurício %A Dias Ribeiro, Carla Caroline %A Willamys Duarte, Joselmo %A Pitelli Boiago Gollucke, Andréa %A Artigiani-Neto, Ricardo %A Araki Ribeiro, Daniel %A Jankiel Miszputen, Sender %A Tizuko Fujiyama Oshima, Celina %A Ribeiro Paiotti, Ana Paula %T Effect of Concentrated Apple Extract on Experimental Colitis Induced by Acetic Acid %J International Journal of Molecular and Cellular Medicine %V 6 %N 1 %U http://ijmcmed.org/article-1-619-en.html %R 10.22088/acadpub.BUMS.6.1.38 %D 2017 %K Experimental colitis, apple extract, antioxidant, iNOS, CuZnSOD, %X Reactive oxygen and nitrogen species (ROS/RNS) play a crucial role in inflammatory bowel disease (IBD) exacerbating the chronic inflammatory process. Endogenous and diet antioxidants can neutralize these compounds. The apple is widely consumed, with several antioxidant activity compounds. The present study evaluated the effects of concentrated apple extract (CAE) in acetic acid induced colitis. 29 Wistar male rats were randomized into 5 groups. G1 – Sham/saline solution, G2 – CAE/control, G3 – acetic acid/control, G4 – curative-CAE treatment and G5 – preventive-CAE treatment. 8 days later, the animals were euthanized and the colonic segment resected for macroscopic and histological analysis. Gene expression was evaluated for inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), catalase and copper and zinc superoxide dismutase (CuZnSOD) by quantitative real time PCR, while protein expression was assessed for iNOS, COX-2 and 8-hydroxy-20-deoxyguanosine (8-OHdG) by immuno-histochemistry. The groups G3, G4 and G5 had weight loss, while G5 had weight increase at the end of the experiment. The treatment with CAE reduced the macroscopic and microscopic injury, decreased iNOS mRNA expression and increased CuZnSOD mRNA expression in animals with induced acetic acid-colitis. The findings of the present study suggest that CAE treatment exerts an antioxidant role by downregulating iNOS and upregulating CuZnSOD. %> http://ijmcmed.org/article-1-619-en.pdf %P 38-49 %& 38 %! Effect of Apple Extract on Colitis %9 Original Article %L A-10-954-2 %+ Department of Pathology, Universidade Federal de São Paulo, Escola Paulista de Medicina, São Paulo, Brazil. %G eng %@ 2251-9637 %[ 2017