:: Volume 2, Issue 2 (Int J Mol Cell Med 2013) ::
Int J Mol Cell Med 2013, 2(2): 64-71 Back to browse issues page
Screening of antibacterial producing actinomycetes from sediments of the Caspian Sea
Mojtaba Mohseni1, Hamed Norouzi2, Javad Hamedi3, Aboulghasem Roohi4
1- Department of molecular and cellular biology, University of Mazandaran, Babolsar, Iran. , M.Mohseni@umz.ac.ir
2- Department of molecular and cellular biology, University of Mazandaran, Babolsar, Iran.
3- Department of microbiology, University of Tehran, Tehran, Iran.
4- Caspian Sea Ecology Research Institute, Sari, Iran.
Abstract:   (18635 Views)
Actinomycetes are interesting as a main producer of secondary metabolites and industrial antibiotics from marine environments. A total of 44 strains of actinomycetes were isolated from Caspian Sea sediments at a depth of 5-10 m. Preliminary screening was done using cross-streak method against 2 gram-positive and 4 gram-negative pathogen bacteria. The most potent strains MN2, MN3, MN38, MN39, MN40, MN41, and MN44 were used to extract the antibacterial substances. The antibacterial activities were performed using Kirby-Bauer disk diffusion method. Potent actinomycetes were screened for hydrolytic exoenzymatic activities (amylase and protease). All of the 24 isolates were active against at least to one of the test organisms. The MN38 strain showed activity against Staphylococcus aureus (20.0±0.5mm), Bacillus subtilis (27.0±0.2 mm), and Escherichia coli (20.0±0.3 mm). The MN39 strain was also active against E. coli (23.0±0.4mm), B. subtilis (23.0±0.2mm), Klebsiella pneumonia (24±0.1mm), whereas, the MN3 strain showed activity against Pseudomonas aeruginosa (20.0±0.2mm). The results of this investigation revealed that the marine actinomycetes of Caspian Sea sediments were potent source of novel antibiotics and bioactive compounds.
Keywords: Marine actinomycetes, antimicrobial activity, caspian Sea
Full-Text [PDF 206 kb]   (6684 Downloads)    
Type of Study: Original Article | Subject: Medical Pharmacology
Received: 2013/05/1 | Accepted: 2013/07/13 | Published: 2013/07/13


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Volume 2, Issue 2 (Int J Mol Cell Med 2013) Back to browse issues page