:: Volume 7, Issue 1 (Int J Mol Cell Med 2018) ::
Int J Mol Cell Med 2018, 7(1): 32-43 Back to browse issues page
Epidemiology of Infantile Visceral Leishmaniasis in Western Algerian And The Convenience of Serum For The Disease Diagnosis by PCR and Immunochromatography
Touria Hadj-Slimane 1, Kheira Senouci2, Nori Midoun3, Assia Bouchetara4, Amel Laradj4, Fadi Bittar5
1- Natural and Life Sciences Faculty, Department of Biology, University of Oran 1 Ahmed Ben Bella, Oran, Algeria. , hadjslimanetouria@gmail.com
2- Natural and Life Sciences Faculty, Department of Biology, University of Oran 1 Ahmed Ben Bella, Oran, Algeria.
3- Department of Epidemiology and Preventive Medicine, University Hospital of Oran (EHU), Oran, Algeria.
4- Infectious Diseases Department, Pediatric Hospital of Oran (EHS), Oran, Algeria.
5- Aix-Marseille University- Faculty of Pharmacy, IHU-Méditerranée Infection, Marseille, France.
Abstract:   (4455 Views)
Epidemiological situation of infantile visceral leishmaniasis (IVL), which is a public health problem in Algeria, is almost unknown in the cities of Western part of the country. The aim of this study was to analyze the epidemiological, clinical, biological, therapeutic, and evolutionary aspects of IVL in Western Algeria, to evaluate the performance of the immunochromatography as a rapid diagnostic test of the disease, and to propose a diagnosis approach by real-time polymerase chain reaction (RT-PCR) assay from the serum. This prospective study was performed on 63 suspicious cases of visceral leishmaniasis collected from the infectious diseases department at the Pediatric Hospital of Oran from January 2012 to July 2017. For each patient, the epidemiological parameters, and the clinical and biological data were collected. Bone marrow and blood samples were drawn from all cases. Bone marrow was performed to research amastigote forms of Leishmania and to identify the species by PCR-sequencing. Blood samples were used to detect anti-Leishmania antibodies as well as parasite DNA. Patients from the Western regions were mostly from rural areas. Sensitivity of RT-PCR from the bone marrow and from serum was 95.45% and 94.44%, respectively. The immunochromatography allowed the disease’s diagnosis for 11 cases whose myelogram did not confirm the presence of the amastigote forms of Leishmania. Immunochromatography was revealed to be a good technique for disease diagnosis regarding the strongly evocative clinical signs. The results also suggest the interest of the RT-PCR assay from patient serum as a non-invasive sample, in the detection of parasite DNA.
Keywords: Imunochromatography, RT-PCR, bone marrow, serum, sequencing, Leishmania infantum
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Type of Study: Original Article | Subject: Infectious disease (Molecular and Cellular aspects)
Received: 2017/12/2 | Accepted: 2018/02/14 | Published: 2018/03/14

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