Expression Pattern of Neuronal Markers in PB-MSCs Treated by Growth Factors Noggin, bFGF and EGF
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Zahra Fazeli1 , Masoumeh Rajabibazl1 , Siamak Salami2 , Nader Vazifeh Shiran2 , Sayyed Mohammad Hossein Ghaderian3 , Mir Davood Omrani 4 |
1- Department of Medical Genetics, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran. 2- Department of Clinical Biochemistry, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran. 3- Department of Hematology, School of Allied Medical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran. 4- Department of Medical Genetics, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran. , davood_omrani@yahoo.co.uk |
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Abstract: (10064 Views) |
Mesenchymal stem cells (MSCs) have the ability to differentiate into neuronal like cells under appropriate culture condition. In this study, we investigated whether MSCs derived from human peripheral blood (PB-MSCs) can differentiate into neuronal like cells by synergic effect of the growth factors EGF, bFGF and Noggin. For this purpose, the expression of five neuronal markers (Nestin, &beta III tubulin, NFM, MAP2 and NSE) were evaluated in treated PB-MSCs by SYBR Green Real time PCR. The expression analysis showed a higher expression of &beta- tubulin and NFM in treated BP-MSCs compared with untreated PB-MSCs as a control group. The expression of Nestin was also diminished in PB-MSCs treated with Noggin. This study suggested that the treatment of PB- MSCs with Noggin alongside with bFGF and EGF might differentiate these cells into neuronal lineage cells. The obtained results could be further developed for useful applications in regenerative medicine. |
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Keywords: Mesenchymal Stem Cells, Differentiation, Neuronal markers, Noggin |
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Full-Text [PDF 158 kb]
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Type of Study: Original Article |
Subject:
Stem Cell Received: 2015/09/15 | Accepted: 2015/10/13 | Published: 2015/12/26
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