Gold nanoparticles (AuNPs) are a suitable choice in the biomedical field due to their physical properties. The presence of gold nanoparticles, acting alongside mesenchymal stem cells (MSCs) through paracrine interactions, accelerates wound closure, enhances angiogenesis, favorably regulates extracellular matrix remodeling, and encourages skin regeneration with typical construction and function. In this study, we hypothesized that AuNPs Nano scaffolds would enhance the proliferation and specialization potential of human adipose-derived mesenchymal stem cells (hADSC in the wound area during in vitro, In vivo studies. To evaluate the nanoparticles; transmission electron microscopy was used to detect AuNPs and dynamic light scattering assay was used to estimate their size. Immunofluorescence staining, MTT assay, and semi-quantitative histological analysis were performed. Our results show that AuNPs at non-toxic concentrations (6 ppm) improve collagen content deposition and micro vessels expression induces cell migration and therapeutic efficacy in wound healing by hADSC at 14 and 28 days after surgery. As a result, AuNPs promote differentiation through a paracrine signaling pathway. Therefore, hADSC cultured with AuNPs can be used as a reagent to enhance skin tissue formation.