:: Volume 11, Issue 4 (Int J Mol Cell Med 2022) ::
Int J Mol Cell Med 2022, 11(4): 274-284 Back to browse issues page
FN14 mRNA Expression Correlates with an Increased Number of Veins during Angiogenesis in the Process of Liver Fibrosis
Elena Lebedeva1, Andrei Babenka2, Pelin Hastemir3, Anatoliy Shchastniy4, Dmitry Zinovkin5, Md Zahidul Islam Pranjol3
1- Department of Histology, Vitebsk State Medical University, Vitebsk, Belarus.
2- Department of Bioorganic Chemistry, Belarussian State Medical University, Minsk, Belarus.
3- School of Life Sciences, University of Sussex, Brighton, UK.
4- Department of Hospital Surgery, Vitebsk State Medical University, Vitebsk, Belarus.
5- Department of Pathology, Gomel State Medical University, Gomel, Belarus. , zinovkin_da@gsmu.by
Abstract:   (650 Views)

In this study, we hypothesize that angiogenesis of special hepatic vessels such as sinusoid capillaries or veins is closely associated with increasing production of connective tissue in fibrogenesis. 36 male Wistar rats were induced with hepatitis and cirrhosis of the liver using thioacetamide. The number of sinusoidal capillaries, veins, arteries and the area of connective tissue were counted and determined. Immunohistochemical study was performed on paraffin sections using monoclonal mouse anti-CD31. mRNA expression was determined using qPCR. We found a statistically significant reduction in the number of sinusoidal capillaries (p<0.0001) and an increase in the number of interlobular veins (p<0.0001) in the fibrosis and cirrhosis groups compared to the control group. There are no differences in the number of interlobular arteries (p=0.282) in the three groups. In our analysis, we found that the expression (mRNA) of Fn14 correlated with the number of veins in liver fibrosis (r=0.44, p=0.008). Our data shows that modulation of veins angiogenesis during fibrosis in chronic liver diseases may play an important role in increasing pathological changes of the liver.

Keywords: Angiogenesis, fibrosis, cirrhosis, FN14, liver
Full-Text [PDF 893 kb]   (168 Downloads)    
Type of Study: Original Article | Subject: Cell Biology
Received: 2022/08/23 | Accepted: 2023/07/15 | Published: 2023/07/15

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Volume 11, Issue 4 (Int J Mol Cell Med 2022) Back to browse issues page