:: Volume 11, Issue 3 (Int J Mol Cell Med 2022) ::
Int J Mol Cell Med 2022, 11(3): 223-235 Back to browse issues page
Increased Expression of Tight Junction Proteins and Blood-Brain Barrier Integrity in MCAO Rats Following Injection of miR-149-5p
Meysam Forouzandeh1 , Hossein Mostafavi2 , Elham Ghasemloo3 , Parvin Mohammadi4 , Masoumeh Hosseini5 , Mehdi Eskandari 6
1- Faculty of Life Sciences, Shahid-Beheshti University, Tehran, Iran.
2- Cancer Gene Therapy Research Center, Zanjan University of Medical Sciences, Zanjan, Iran.
3- Department of Physiology, School of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran.
4- Department of Medical Biotechnology, School of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran.
5- Laboratory Expert, Depertment of Physiology, School of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran.
6- Cancer Gene Therapy Research Center, Zanjan University of Medical Sciences, Zanjan, Iran. , mehdiesk@zums.ac.ir
Abstract:   (817 Views)

Cerebral ischemia is a common neurodegenerative disease in which damage to the blood-brain barrier (BBB) is the main consequence. In cerebral ischemia, the level of miR-149-5p and tight junction proteins are decreased, while the level of Calpine is increased, finally leading to increased BBB permeability. This study investigated the effect of miR-149-5p mimic on the expression of Calpain, Occludin, and ZO-1 and the consequences of cerebral ischemia. Cerebral ischemia model was performed via middle cerebral artery occlusion (MCAO) method on female Wistar rats. Four groups of Wistar rats were studied: Sham, cerebral ischemia without treatment, Scramble miR, and miR-149-5p mimic treatment. Then, neurological defects and BBB permeability (via Evans blue staining), cerebral edema (cerebrospinal fluid percentage), and ZO-1, Occludin, and Calapin expression (by quantitative real time- PCR) were investigated. qRT-PCR results showed miR-149-5p expression decreases after cerebral ischemia induction. In addition, Occludin and ZO-1 expression significantly increased in miR-149-5p group. In contrast, Calapin expression, BBB permeability, brain water content and neurological defects were significantly decreased. It seems that the increased level of miR-149-5p exerts its protective effect on cerebral ischemia due to increasing of tight junction proteins.

Keywords: Cerebral ischemia, middle cerebral artery occlusion, miR-149-5p, tight junction
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Type of Study: Original Article | Subject: Neurosciences
Received: 2022/08/16 | Accepted: 2023/07/9 | Published: 2023/07/9



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Volume 11, Issue 3 (Int J Mol Cell Med 2022) Back to browse issues page