:: Volume 11, Issue 2 (Int J Mol Cell Med 2022) ::
Int J Mol Cell Med 2022, 11(2): 150-167 Back to browse issues page
miR-33-5p Regulates CREB to Induce Morphine State-dependent Memory in Rats: Interaction with µ Opioid Receptor
Sadegh Moradi-Vastegani1 , Behrang Alani2 , Khatereh Kharazmi1 , Abolfazl Ardjmand 3
1- Institute for Basic Sciences, Physiology Research Center, Kashan University of Medical Sciences, Kashan, Iran.
2- Department of Applied Cell Sciences, School of Medicine, Kashan University of Medical Sciences, Kashan, Iran.
3- Institute for Basic Sciences, Physiology Research Center, Kashan University of Medical Sciences, Kashan, Iran. , ardjmand_ab@kaums.ac.ir
Abstract:   (1337 Views)

The aim of the present study was to examine the hypothesis that miR-33-5p attenuates morphine state-dependent (StD) memory via the µ opioid receptor by regulating cyclic AMP response element-binding protein (CREB). The effects of post-training morphine and morphine StD memory and their interaction with pre-test naloxone were evaluated using a single-trial inhibitory avoidance paradigm. Then, the hippocampal miR-33-5p gene and pCREB/CREB protein expression profiles were evaluated using quantitative real-time PCR and western blotting, respectively. We found that while post-training morphine and morphine StD memory respectively up- and down-regulate the miR-33-5p expression profile in the hippocampus, the reverse results are true for the expression of pCREB/CREB. Pre-test naloxone antagonized the response. Overall, our findings suggest that the expression levels of miR-33-5p in the hippocampus set the basis for morphine StD memory with low miR-33-5p enabling state dependency. The mechanism is mediated via miR33-5p and CREB signaling with the interaction of the µ opioid receptor. This finding may be used as a potential strategy for ameliorating morphine-induced memory-related disorders.

Keywords: miR-33-5p, CREB, state-dependent memory, opioids, rats
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Type of Study: Original Article | Subject: Neurosciences
Received: 2022/05/25 | Accepted: 2023/01/21 | Published: 2023/02/1



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Volume 11, Issue 2 (Int J Mol Cell Med 2022) Back to browse issues page