miR-33-5p Regulates CREB to Induce Morphine State-dependent Memory in Rats: Interaction with µ Opioid Receptor
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Sadegh Moradi-Vastegani1 , Behrang Alani2 , Khatereh Kharazmi1 , Abolfazl Ardjmand 3 |
1- Institute for Basic Sciences, Physiology Research Center, Kashan University of Medical Sciences, Kashan. 2- Department of Applied Cell Sciences, School of Medicine, Kashan University of Medical Sciences, Kashan. 3- Institute for Basic Sciences, Physiology Research Center, Kashan University of Medical Sciences, Kashan. , ardjmand_ab@kaums.ac.ir |
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Abstract: (520 Views) |
The aim of the present study was to examine the hypothesis that miR-33-5p attenuates morphine state-dependent (StD) memory via the µ opioid receptor by regulating cyclic AMP response element-binding protein (CREB). The effects of post-training morphine and morphine StD memory and their interaction with pre-test naloxone were evaluated using a single-trial inhibitory avoidance paradigm. Then, the hippocampal miR-33-5p gene and pCREB/CREB protein expression profiles were evaluated using quantitative real-time PCR and western blotting, respectively. We found that while post-training morphine and morphine StD memory respectively up- and down-regulate the miR-33-5p expression profile in the hippocampus, the reverse results are true for the expression of pCREB/CREB. Pre-test naloxone antagonized the response. Overall, our findings suggest that the expression levels of miR-33-5p in the hippocampus set the basis for morphine StD memory with low miR-33-5p enabling state dependency. The mechanism is mediated via miR33-5p and CREB signaling with the interaction of the µ opioid receptor. This finding may be used as a potential strategy for ameliorating morphine-induced memory-related disorders. |
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Keywords: miR-33-5p, CREB, state-dependent memory, opioids, rats |
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Full-Text [PDF 584 kb]
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Type of Study: Original Article |
Subject:
Neurosciences Received: 2022/05/25 | Accepted: 2023/01/21 | Published: 2023/02/1
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