International Journal of Molecular and Cellular Medicine (IJMCM)
Cellular and Molecular Biology Research Center, Babol University of Medical Sciences
پنجشنبه 1 آذر 1403
[Archive]
دوره 11، شماره 2 - ( 9-1401 )
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Prakash A, Das Purkayastha B P, Srivastava S, Chaturvedi S, Ali A, Aggarwal D D et al . Novel Variant Identified in the Enhancer Region of Host Transcription Factor, BRN3A, is a Significant Risk Factor for HPV-Induced Uterine Cervix Cancer. Int J Mol Cell Med 2022; 11 (2) :88-103
URL: http://ijmcmed.org/article-1-1842-fa.html
URL: http://ijmcmed.org/article-1-1842-fa.html
Novel Variant Identified in the Enhancer Region of Host Transcription Factor, BRN3A, is a Significant Risk Factor for HPV-Induced Uterine Cervix Cancer. مجله بین المللی سلولی و مولکولی. 1401; 11 (2) :88-103
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Among HPV-mediated cervical cancers, cellular factor BRN3A has gained considerable attention due to its role in promoting an anti-apoptotic cellular environment and in facilitating epitheliotropic transformations of the host. The majority of previous studies looked at BRN3A's molecular characteristics; however, the possibility of genetic variations in BRN3A's auto-regulatory region in relation to cervical cancer risk has been underestimated until now. In a retrospective study in the Eastern UP population, India, we detected genetic variations in the cis-regulatory proximal enhancer region located around 5.6 kb upstream of transcription start site of BRN3A. Our analysis of PCR and DNA sequencing confirmed this novel SNP (BRN3A g.60163379A>G) within the auto-regulatory region of BRN3A. As compared to control subjects, cancer cases exhibited a 1.32-fold higher allele frequency (χ2 = 6.315, p = 0.012). In homozygous (GG) but not in heterozygous conditions, odds ratio (OR) analysis suggests a significant association of cancer risk with the SNP (OR = 2.60, p ≤ 0.004). We further confirmed using the functional analysis that this SNP increased the luciferase gene activity in HPV-positive cervical cancer SiHa cells that were exposed to progesterone. As a result of the association of polymorphisms in a non-coding region of an oncogene with increased cancer risks, we are suggesting that this genetic variation in non-coding region can be used in prediction, diagnosis, or predicting the progression of the disease.
نوع مطالعه: Original Article |
موضوع مقاله:
Cancer
دریافت: 1400/10/26 | پذیرش: 1401/9/20 | انتشار: 1401/9/21
دریافت: 1400/10/26 | پذیرش: 1401/9/20 | انتشار: 1401/9/21
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