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Mesenchymal Stem Cells cause Telomere Length Reduction of Molt-4 Cells via Caspase-3, BAD and P53 Apoptotic Pathway
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Hamid Reza Heidari1    , Ezzatollah Fathi2 , Soheila Montazersaheb3 , Ayoub Mamandi4 , Raheleh Farahzadi 5, Soran Zalavi4 , Hojjatollah Nozad Charoudeh6 |
1- Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
2- Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran.
3- Molecular Medicine Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
4- Student Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran.
5- Hematology and Oncology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran. , farahzadir@tbzmed.ac.ir
6- Department of Anatomical Sciences, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran. |
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Abstract: (3206 Views) |
| Mesenchymal stem cells (MSCs) as undifferentiated cells are specially considered in cell-based cancer therapy due to unique features such as multi-potency, pluripotency, and self-renewal. A multitude of cytokines secreted from MSCs are known to give such multifunctional attributes, but details of their role are yet to be unknown. In the present study, MSCs were cultured, characterized and co-cultured with Molt-4 cells as acute lymphoblastic leukemia cell line in a trans-well plate. Then, cultured Molt-4 alone and Molt-4 co-cultured with MSCs (10:1) were collected on day 7 and subjected to real time-PCR and Western blotting for gene and protein expression assessment, respectively. Ki-67/caspase-3 as well as telomere length were investigated by flow cytometry and real time-PCR, respectively. The results showed that MSCs caused significant decrease in telomere length as well as hTERT gene expression of Molt-4 cells. Also, gene and protein expression of BAD and P53 were significantly increased. Furthermore, the flow cytometry analysis indicated the decrease and increase of the Ki-67 and caspaspase-3 expression, respectively. It was concluded that MSCs co-cultured with Molt-4 cells could be involved in the promotion of Molt-4 cell apoptosis via caspase-3, BAD, and P53 expression. In addition, the decrease of telomere length is another effect of MSCs on Molt-4 leukemic cells. |
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| Keywords: Mesenchymal stem cells, telomere length, hTERT, BAD, P53, caspase-3, apoptotic pathway |
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Full-Text [PDF 639 kb]
(1117 Downloads)
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Type of Study: Original Article |
Subject:
Stem Cell
Received: 2021/01/13 | Accepted: 2021/07/24 | Published: 2021/05/30
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