A Cardiac Troponin T Biosensor Based on Aptamer Self-assembling on Gold
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Masoud Negahdary1 , Mostafa Behjati-Ardakani2 , Hossein Heli3 , Naghmeh Sattarahmady 4 |
1- Nanomedicine and Nanobiology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran; Yazd Cardiovascular Research Center, Shahid Sadoughi University of Medical Sciences, Yazd, Iran. 2- Yazd Cardiovascular Research Center, Shahid Sadoughi University of Medical Sciences, Yazd, Iran. 3- Nanomedicine and Nanobiology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran. 4- Nanomedicine and Nanobiology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran; Department of Medical Physics, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran. , nsattar@sums.ac.ir |
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Abstract: (4298 Views) |
In this study, a sensitive and accurate aptasensor was designed for early detection of myocardial infarction through the determination of troponin T (TnT). The successful immobilization of a specific aptamer sequence on the surface of gold that had a high affinity toward TnT was accomplished. TnT was electrochemically quantified. The results indicated that the aptasensor detected TnT in a range of 0.05-5 ng mL and with a detection limit of 0.01 ng/mL. The performance of the aptasensor was investigated by analyzing 99 human serum samples. Both diagnostic specificity and sensitivity of the aptasensor were found to be 95%. The use of the designed aptamer-based biosensor could be an essential achievement in health policy, preventing deaths caused by myocardial infarction, and reducing patients with heart failure. The extensive use of this aptamer-based biosensor can also reduce costs, enhance speed, and improve accuracy in the diagnosis of TnT as an important myocardial infarction biomarker. |
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Keywords: Myocardial infarction, aptasensing, biomarker, bioelectrochemical detection |
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Full-Text [PDF 300 kb]
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Type of Study: Original Article |
Subject:
Biomarkers (diagnosis & treatment) Received: 2019/12/28 | Accepted: 2020/04/13 | Published: 2020/05/29
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