en Biomarkers (diagnosis & treatment) Biomarkers (diagnosis & treatment) Original Article Original Article <p><span style="font-size:12px;"><span style="font-family:Tahoma;"><span style="line-height:107%">Early detection is crucial for improving survival rates in colorectal cancer (CRC). This study evaluates the non-invasive diagnosis of polyps by assessing the methylation status of the TFPI2 and SDC2 genes in plasma. This study enrolled 27 individuals with low-risk polyps (LRP), 27 with high-risk polyps (HRP), and 27 healthy controls. The quantitative methylation levels of TFPI2 and SDC2 genes were analyzed in plasma cell-free DNA (cfDNA) using the methylation-quantification endonuclease-resistant DNA (MethyQESD) method. Increased methylation percentages of both TFPI2 (TFPI2_1 and TFPI2_2) and SDC2 (SDC2_2) were observed in individuals with LRP and HRP. The combination of SDC2 and TFPI2 yielded an Area Under the Curve (AUC) of 0.732 (95% CI 0.78 to 0.96, p=0.001) with a sensitivity of 66% (95% CI 46% - 82%) and specificity of 77% (95 CI 56% - 91%) for LRP. For HRP, the AUC was 0.890 (95% CI 0.596 to 0.843, p<0.001) with a sensitivity of 70% (95% CI 51% - 84%) and specificity of 92% (95 CI 75% - 99%). The combined assessment of SDC2 and TFPI2 methylation presents a potential approach for the early non-invasive detection of CRC and its associated precancerous lesions</span></span></span></p> Colorectal cancer, Methylation, plasma, polyp, SDC2, TFPI2 646 655 http://ijmcmed.org/browse.php?a_code=A-10-8194-1&slc_lang=en&sid=1 Milad Khabbazpour m.khabbazpour@gmail.com 100319475328460034594 100319475328460034594 No Student Research Committee, Baqiyatallah University of Medical Sciences, Tehran, Iran Mohammad Heiat mohamad.heiat@gmail.com 100319475328460034595 100319475328460034595 No Baqiyatallah Research Center for Gastroenterology and Liver Diseases (BRCGL), Clinical Sciences Institute, Baqiyatallah University of Medical Sciences, Tehran, Iran Ashraf Karbasi ashraf.karbasi@yahoo.com 100319475328460034596 100319475328460034596 No Baqiyatallah Research Center for Gastroenterology and Liver Diseases (BRCGL), Clinical Sciences Institute, Baqiyatallah University of Medical Sciences, Tehran, Iran Majid Zaki-Dizaji majid.zaki-dizaji@gmail.com 100319475328460034597 100319475328460034597 Yes Human Genetics Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran