1 2251-9637 Babol University of Medical Sciences 67 Biomarkers (diagnosis & treatment) Cellular response to ionizing radiation: A microRNA story Halimi Mohammad b Asghari Mohsen c Sariri Reyhaneh d Moslemi Dariush e Parsian Hadi f b Department of Biology, Faculty of Sciences, University of Guilan, Rasht, Iran. c Department of Biology, Faculty of Sciences, University of Guilan, Rasht, Iran. d Department of Biology, Faculty of Sciences, University of Guilan, Rasht, Iran. e Department of Radiation oncology, Babol University of Medical Sciences, Babol, Iran. f Cellular and Molecular Biology Research Center, Babol University of Medical Sciences, Babol, Iran. 1 12 2012 1 4 178 184 11 02 2013 03 07 2013 MicroRNAs (miRNAs) represent a class of small non-coding RNA molecules that regulate gene expression at the post-transcriptional level. They play a crucial role in diverse cellular pathways. Ionizing radiation (IR) is one of the most important treatment protocols for patients that suffer from cancer and affects directly or indirectly cellular integration. Recently it has been discovered that microRNA-mediated gene regulation interferes with radio-related pathways in ionizing radiation. Here, we review the recent discoveries about miRNAs in cellular response to IR. Thoroughly understanding the mechanism of miRNAs in radiation response, it will be possible to design new strategies for improving radiotherapy efficiency and ultimately cancer treatment.
60 Cell Biology Rab11 is required for maintenance of cell shape via βPS integrin mediated cell adhesion in Drosophila Bhuin Tanmay g Kumar Roy Jagat h g Cytogenetics Laboratory, Department of Zoology, Banaras Hindu University, Varanasi 221 005, India. h Cytogenetics Laboratory, Department of Zoology, Banaras Hindu University, Varanasi 221 005, India. 1 12 2012 1 4 185 190 28 01 2013 18 03 2013 In eukaryotes, vesicle trafficking is regulated by the small monomeric GTPases of the Rab protein family. Rab11, (a subfamily of the Ypt/Rab gene family) an evolutionarily conserved, ubiquitously expressed subfamily of small monomeric Rab GTPases, has been implicated in regulating vesicular trafficking through the recycling of endosomal compartment. In an earlier communication, we have shown that Rab11 is required for cell adhesion, maintenance of cell shape and actin-cytoskeleton organization during Drosophila wing development. Here, we report that Rab11 is required for the maintenance of cell shape via βPS integrin mediated cell adhesion. Cuticle preparations of the embryos, when Rab11 is over-expressed or activity of Rab11 is reduced via a double-stranded RNAi line, show dorsal open phenotypes. Immuno-fluorescence and immuno-histochemical analyses on embryos in the same genetic backgrounds also affect the localization of βPS integrins from the adhesion site of leading edge and amnioserosa cells during the dorsal closure stages of embryogenesis as well as the cellular morphology (cell shape) of the lateral epidermal cells. 71 Clinical Biochemistry Central effects of camphor on GnRH and sexual hormones in male rat shahabi sima i Jorsaraei Seyed Gholam Ali j Moghadamnia Ali Akbar k Zabihi Ebrahim l Aghajanpour Mir Seyed Mohsen m Mousavi Kani Seyedeh Narges n Pourbagher Roghiyeh o Hosseini Seyed Ahmad p Esmaili Mohsen Yoonesi Ali Asghar Zarghami Amin Alinezhad Farid i Cellular and Molecular Biology Research Center (CMBRC), Babol University of Medical Sciences, Babol, Iran. j Fatemeh Zahra Fertility and Infertility Reproductive Health Research Center, Babol University of Medical Sciences, Babol, Iran. k Department of Physiology and Pharmacology, Babol University of Medical Sciences, Babol, Iran. l Cellular and Molecular Biology Research Center (CMBRC), Babol University of Medical Sciences, Babol, Iran. m Cellular and Molecular Biology Research Center (CMBRC), Babol University of Medical Sciences, Babol, Iran. n Cellular and Molecular Biology Research Center (CMBRC), Babol University of Medical Sciences, Babol, Iran. o Cellular and Molecular Biology Research Center (CMBRC), Babol University of Medical Sciences, Babol, Iran. p Student Research Committee, Babol University of Medical Sciences, Babol, Iran. Student Research Committee, Babol University of Medical Sciences, Babol, Iran. Student Research Committee, Babol University of Medical Sciences, Babol, Iran. Student Research Committee, Babol University of Medical Sciences, Babol, Iran. Student Research Committee, Babol University of Medical Sciences, Babol, Iran. 1 12 2012 1 4 191 196 18 02 2013 11 05 2013 In Persian traditional medicine is believed that camphor (a crystalline ketone obtained from cinnamomum camphora) is a suppressor of sexual behaviors. This study examined the central effects of camphor on sexual hormones (LH, FSH and testosterone) and GnRH plasma levels in male rat. Male Wistar rats weighing 250-260gr were selected and divided into control (no treatment), sham (ICV injection of EtOH 10%) and treatment (ICV injection of camphor in three doses 4, 20, 40 µg/ 10µl in alcohol) groups. The serum samples were used for assaying of GnRH, LH, FSH and testosterone. There were no significant differences in the levels of hormones between the groups of study. Despite the central administration of camphor in hypothalamus - pituitary - gonad (HPG) axis, no significant differences were seen in sex hormone`s levels compared to the control. With this finding, it can be concluded that camphor may not effectively handle the axis via central pathway. These data recommend further studies of camphor on the HPG axis. 63 Cell Biology A comparison between cytotoxicity induced by two resin based sealers (2Seal and AH Plus) in Saos-2 and MG-63 cell lines Ehsani Maryam Zabihi Ebrahim Gharouee Hamed Dental Materials Research Center, School of Dentistry, Babol University of Medical Sciences, Babol, Iran. Cellular and Molecular Biology Research Center, Babol University of Medical Sciences, Babol, Iran. Department of Endodontics, School of Dentistry, Babol University of Medical Sciences, Babol, Iran. 1 12 2012 1 4 197 202 02 02 2013 09 04 2013 The aim of this study was to evaluate and compare the cytotoxicity induced by two resin-based sealers, 2Seal and AH Plus, in two osteoblast-like cell lines, MG-63 and Saos-2. Using sterile discs of both sealers in complete media, 24- and 72-h extracts were prepared. The extracts were exchanged with Saos-2 or MG-63 cell culture media at 75% confluence, and after 24 h incubation, cell viability tests were performed for each extract and cell line using MTT and trypan blue dye exclusion assays. Corresponding incubated media were used as negative control groups. For both extracts and sealers, cytotoxicity was observed in both cell lines. For Saos-2, there was no statistical difference in toxicity between the sealers for either extract (p > 0.05). For MG-63, the 2Seal 24-h extract and the AH Plus 72-h extract had greater cytotoxicity than the other extracts (p < 0.05(. Both AH Plus and 2Seal demonstrated significant cytotoxicity in these two cell lines. In contrast to 2Seal, the cytotoxicity of AH Plus in the MG-63 cell line increased with extraction time from 24 to 72 h. The AH Plus and 2Seal 24-h extracts showed different levels of cytotoxicity in the MG-63 cell line, while in the Saos-2 cell line there were no detectable differences. This may reflect higher sensitivity of the MG-63 cell line compared to Saos-2 toward cytotoxicity induced by these two sealers, or different kinetics of toxicant release from the sealers. 65 Genetics & Disease Evaluation of PKM2 and MAPK8IP2 polymorphism in Ameloblastic Carcinoma: A retrospective quantitative study Khodayari Abbas Ghaderian Sayyed Mohammad Hossein Jafarian Mohammad Jahangirnia Alireza Nayebi Alireza Akhlaghi Fahimeh Taghavi Nasim Akbarzadeh Najar Reza Tabarestani Sanaz Khojasteh Arash Aghabozorg Afjeh Sarah Department of Oral and Maxillofacial Surgery, Dental Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran. Department of Medical Genetics, Shahid Beheshti University of Medical Sciences, Tehran, Iran. Department of Oral and Maxillofacial Surgery, Dental Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran. Department of Oral and Maxillofacial Surgery,Shahid Beheshti University of Medical Sciences, Tehran, Iran. Department of Oral and Maxillofacial Surgery, Shahid Beheshti University of Medical Sciences, Tehran, Iran. Department of Oral and Maxillofacial Surgery, Dental Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran. Department of oral and maxillofacial pathology, dental faculty,Shahid beheshty university of medical science,Tehran, Iran. Department of Genetics, Shahid Beheshti University of Medical Sciences, Tehran, Iran. Department of Genetics, Shahid Beheshti university of Medical Sciences, Tehran,Iran. Department of Oral and Maxillofacial Surgery, Dental Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran. Department of Genetics, Shahid Beheshti University of Medical Sciences, Tehran, Iran. 1 12 2012 1 4 203 208 08 02 2013 17 03 2013 Ameloblastic carcinoma (AC) is a rare malignant epithelial odontogenic tumor that histologically retains the features of ameloblastic differentiation and exhibits cytological features of malignancy in the primary or recurrent tumor. It may develop within a preexisting ameloblastoma or arise de novo or from an odontogenic cyst. Epidemiological evidence shows that human cancer is generally caused by genotoxic factors, genes involved in the susceptibility of cancer, including those involved in metabolism or detoxification of genotoxic environment and those controlling DNA replication. Nowadays, gene polymorphism has an important role in development of malignant tumor. We report a case series study of ameloblastic carcinoma and ameloblastoma to show the role of PKM2 and MAPK8IP2 polymorphisms in these tumors. The DNA was extracted separately from specimens in paraffin sections of the tumor. Polymorphism of these genes was determined by PCR-RFLP (Polymerase Chain Reaction-Restriction fragment length polymorphism) method. The allele distributions of all samples were in Hardy-Weinberg equilibrium. The genotype and allele distribution in these genes were not statistically different between patients and controls. 56 Infectious disease (Molecular and Cellular aspects) Molecular detection of Integron genes and pattern of antibiotic resistance in Pseudomonas aeruginosa strains isolated from intensive care unit, Shahid Beheshti Hospital, North of Iran Moradian Fatemeh Ferdosi Shahandashti Elaheh Moulana Zahra Moradian kouchaksaraei Massomeh Asgharpour Fariba Mojtahedi Ali Rajabnia Ramazan Department of Microbiology Science and Research Branch Islamic Azad University, Guilan, Iran. Department of Microbiology and Immunology, Babol University of Medical Sciences, Babol Iran. Infectious Diseases and Tropical Medicine Research Center, Babol University of Medical Sciences, Babol, Iran. Department of Microbiology Science and Research Branch Islamic Azad University, Fars, Iran. Faculty of Para-Medicine; Babol University of Medical Sciences, Babol, Iran. Department of Microbiology, Faculty of Medicine, Guilan University Campus, Rasht, Iran. Infectious Diseases and Tropical Medicine Research Center, Babol University of Medical Sciences, Babol, Iran. 1 12 2012 1 4 209 216 09 01 2013 27 05 2013 Pseudomonas aeruginosa is one of the most important pathogens that causes nosocomial infections and shows high level of antibiotic resistance. Integrons are one of the transposable elements in bacteria and their role in antibiotic resistance has been well demonstrated. The aim of this study was a molecular characterization of the integron genes and the determination of the resistance or sensitivity pattern to ceftizoxime, ceptazidime. cephotaxim, amikacin, ofloxacin, imipenem, cefepime, ticarcillin, gentamicin, ciprofloxacin, cefazolin and ceftriaxone antibiotics in P. aeruginosa strains isolated from Intensive Care Units (ICU), Shahid Beheshti Hospital, North of Iran. This cross-sectional study was performed from 2011 to 2012. Totally, fifty four P. aeruginosa strains were isolated from ICU at Shahid-Beheshti hospital, Babol, North of Iran. The bacteria were diagnosed based on mobility, pigment production, growth in 420 C, oxidase and catalase tests. PCR analysis was carried out to detect integron genes using hep 35 and hep 36 primers. Also, disk diffusion method was performed to evaluate antibiotic susceptibility of the bacteria using ceftizoxime, ceftazidime, cephotaxime, amikacin, ofloxacin, imipenem, cefepime, ticarcillin, gentamicin, ciprofloxacin, cefazolin and ceftriaxone antibacterial reagents. This study revealed that 20 (37%) P. aeruginosa isolates had integron genes. The antibiotic susceptibility test showed that 52 (96.3%) of the isolates were multidrug-resistant. 12 out of 54 isolated bacteria were resistant to all antibiotics tested. All bacteria were resistant to cefepime (100%) and the highest resistance rate was seen to ceftazidime 92.6% fallowed by cefazolin 96.3%. The lowest resistance rate was observed to ciprofloxacin 38.9%, ofloxacin 44.4%, amikacin 46.3% and ticarcillin 48.1%. According to this study, P. aeruginosa isolates showed high level of antibiotic resistance and the presence of integrons in these strains can explain the influence of these genes in resistance creation. There was a significant association between resistance to cefotaxime, amikacin, ofloxacin, imipenem, ticarcillin, gentamicin and the presence of integrons. 53 Cancer Relationship of Podoplanin and Glutathione S-transferases T1 Expression with Laryngeal Cancer Kanliada Deniz Coskunpinar Ender Orhan Kadir Serkan Musteri Oltulu Yasemin Celik Mehmet Eren Ayse Yaylim Ilhan Değer Kemal Department of ORL and Head&Neck Surgery, Istanbul Faculty of Medicine, Istanbul, Turkey. Department of Molecular Medicine, Institute of Experimental Medicine, Istanbul University, Istanbul, Turkey. Department of ORL and Head&Neck Surgery, Istanbul Faculty of Medicine, Istanbul, Turkey. Department of Molecular Medicine, Institute of Experimental Medicine, Istanbul University, Istanbul, Turkey. Department of ORL and Head&Neck Surgery, Istanbul Faculty of Medicine, Istanbul, Turkey. Department of Molecular Medicine, Institute of Experimental Medicine, Istanbul University, Istanbul, Turkey. Department of Molecular Medicine, Institute of Experimental Medicine, Istanbul University, Istanbul, Turkey. Department of ORL and Head&Neck Surgery, Istanbul Faculty of Medicine, Istanbul, Turkey. 1 12 2012 1 4 217 224 13 12 2012 17 03 2013 The aim of this study is to determine whether there is a role of podoplanin and glutathione S-transferases T1 (GST-T1) expression in laryngeal squamous cell carcinoma. The study was completed with 33 patients and gene expression analysis was performed by qRT–PCR. The podoplanin and GST-T1 expression patterns were analyzed to determine their correlation with clinicopathologic parameters of laryngeal cancer. Of all patients, 20 had supraglottic, and the remaining 13 had glottic laryngeal cancer. Increased expression of podoplanin was found in 14 tumor tissues, but GST-T1 expression was not detected. Podoplanin expression did not show any prediction for regional metastasis, thyroid cartilage invasion, lymphatic vessel invasion or tumor differentiation for laryngeal cancer, also there were no significant differences in podoplanin expression between glottic and supraglottic regions, but extracapsullar extension is almost statistically significant (p=0,05). 54 Genetics & Disease Balanced Chromosomal Rearrangement in Recurrent Spontaneous Abortions: A Case Report Zarifian Ahmadreza Farhoodi Zeinab Amel Roya Mirzaee Salmahe Hassanzadeh-Nazarabad Mohammad Student Research Assembly, Mashhad University of Medical Sciences, Iran. Student Research Assembly, Mashhad University of Medical Sciences, Iran. Student Research Assembly, Mashhad University of Medical Sciences, Iran. Department of Medical Genetics, Faculty of Medicine, Mashhad University of Medical Sciences, Iran. Department of Medical Genetics, Faculty of Medicine, Mashhad University of Medical Sciences, Iran. 1 12 2012 1 4 225 228 07 01 2013 17 03 2013 One of the major causes of spontaneous abortion before the fourth month of pregnancy is chromosomal abnormalities. We report an unusual case of a familial balanced chromosomal translocation in a consanguineous couple who experienced 4 spontaneous abortions. Chromosomal studies were performed on the basis of G-banding technique at high resolution and revealed 46, XX, t (16 6) (p12 q26) and 46, XY, t (16 6) (p12 q26) in both partners, which induced such pregnancy complications. Chromosomal balanced translocation is one of the most common causes of recurrent spontaneous abortions (RSA). In such cases prenatal diagnosis (PND) during the 16th week of gestation is strongly recommended.