Babol University of Medical Sciences
International Journal of Molecular and Cellular Medicine (IJMCM)
2251-9637
2251-9645
6
2
2017
6
1
The Role of Probiotics in Cancer Treatment: Emphasis on their In Vivo and In Vitro Anti-metastatic Effects
66
76
EN
Elahe
Motevaseli
Department of Molecular Medicine, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran.
e_motevaseli@tums.ac.ir
N
Ali
Dianatpour
Department of Medical Genetics, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
ali.dianatpur@gmail.com
N
Soudeh
Ghafouri-Fard
Department of Medical Genetics, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
s.ghafourifard@sbmu.ac.ir
Y
10.22088/acadpub.BUMS.6.2.1
Probiotics are defined as live bacteria and yeasts that exert beneficial effects for health. Among their various effects, anti-cancer properties have been highlighted in recent years. Such effects include suppression of the growth of microbiota implicated in the production of mutagens and carcinogens, alteration in carcinogen metabolism and protection of DNA from oxidative damage as well as regulation of immune system. We performed a computerized search of the MEDLINE/PUBMED databases with keywords: cancer, probiotics, lactobacilli, metastasis, and invasion. Cell line studies as well as animal models and human studies have shown the therapeutic effects of probiotics in reduction of invasion and metastasis in cancer cells. These results support the beneficial effects of probiotics both in vitro and in vivo. However, pre-clinical or clinical studies are not enough to decide about their application.
probiotics, lactobacilli, cancer, metastasis, invasion
http://ijmcmed.org/article-1-653-en.html
http://ijmcmed.org/article-1-653-en.pdf
Babol University of Medical Sciences
International Journal of Molecular and Cellular Medicine (IJMCM)
2251-9637
2251-9645
6
2
2017
6
1
Circulating miR-92a, miR-143 and miR-342 in Plasma are Novel Potential Biomarkers for Acute Myeloid Leukemia
77
86
EN
Amr Rafat
Elhamamsy
Department of Clinical Pharmacy, School of Pharmacy, Tanta University, 31516 Tanta, Egypt.
amr35354@pharm.tanta.edu.eg
Y
Muhammad Suleiman
El Sharkawy
Department of Clinical Pharmacy, Tanta Cancer Center, 31527 Tanta, Egypt.
muhammad.elsharkawy14@gmail.com
N
Ahmed Farouk
Zanaty
Department of Clinical Pharmacy, Tanta Cancer Center, 31527 Tanta, Egypt.
meropharma147@yahoo.com
N
Mohammed Ahmed
Mahrous
Department of Clinical Pharmacy, School of Pharmacy, Tanta University, 31516 Tanta, Egypt.
Mahrosmohamed14@gmail.com
N
Ahmed
Ezzat Mohamed
Department of Clinical Pharmacy, Tanta Cancer Center, 31527 Tanta, Egypt.
dr_ahmed.ezzat@yahoo.com
N
Eslam Ahmed
Abushaaban
Department of Clinical Pharmacy, Tanta Cancer Center, 31527 Tanta, Egypt.
eslam.aboshaaban@gmail.com
N
10.22088/acadpub.BUMS.6.2.2
MicroRNAs (miRNAs) are small non-coding RNAs that function as post-transcriptional gene expression regulators. The expression profiling of miRNAs has already entered into cancer clinics as diagnostic and prognostic biomarkers to assess tumor initiation, progression and response to treatment in cancer patients. Recent Studies opened the way for the use of circulating miRNAs as non-invasive diagnosis and prognosis of Acute Myeloid Leukemia (AML). The aim of this study was to identify plasma miR-92a, miR-143 and miR-342 expression signatures in AML patients to introduce new markers for establishing AML diagnosis and prognosis. Blood samples were collected from 65 AML patients and 50 controls. The expression of three target miRNAs (miR-92a, miR-143 and miR-342) was measured using quantitative real-time PCR method. Plasma levels of miR-92a, miR-143 and miR-342 were significantly lower in AML patients in comparison with control group. Receiver operator characteristic (ROC) analysis revealed that the sensitivity and specificity values of miR-92a were 81.5% and 94%, respectively, with a cut-off value of 0.704. The sensitivity and specificity values of miR-143 were 87.7% and 80%, respectively, with a cut-off value of 0.65. The sensitivity and specificity values of miR-342 were 75.4% and 90%, respectively, with a cut-off value of 0.479. Our findings suggest that plasma miR-92a, miR-143 and miR-342 could be promising novel circulating biomarkers in clinical detection of AML.
Leukemia, myeloid, acute, diagnosis, microRNAs.
http://ijmcmed.org/article-1-636-en.html
http://ijmcmed.org/article-1-636-en.pdf
Babol University of Medical Sciences
International Journal of Molecular and Cellular Medicine (IJMCM)
2251-9637
2251-9645
6
2
2017
6
1
Evaluation of MicroRNA-99a and MicroRNA-205 Expression Levels in Bladder Cancer
87
95
EN
Sajjad
Mohammad Ganji
Research Center for Molecular Medicine, Department of Genetics and Molecular Medicine, Hamadan University of Medical Sciences, Hamadan, Iran.
sina.m.ganji@gmail.com
N
Massoud
Saidijam
Research Center for Molecular Medicine, Department of Genetics and Molecular Medicine, Hamadan University of Medical Sciences, Hamadan, Iran.
sjam110@yahoo.com
N
Razeyeh
Amini
Research Center for Molecular Medicine, Department of Genetics and Molecular Medicine, Hamadan University of Medical Sciences, Hamadan, Iran.
aminra14@gmail.com
N
Seyed Habibollah
Mousavi-Bahar
Urology and Nephrology Research Center, Shahid Beheshti Hospital, Hamadan University of Medical Sciences, Hamadan, Iran.
shmbahar@yahoo.com
N
Nooshin
Shabab
Research Center for Molecular Medicine, Department of Genetics and Molecular Medicine, Hamadan University of Medical Sciences, Hamadan, Iran.
nnshabab2@gmail.com
N
Saman
Seyedabadi
Research Center for Molecular Medicine, Department of Genetics and Molecular Medicine, Hamadan University of Medical Sciences, Hamadan, Iran.
seyedabadi1987@gmail.com
N
Ali
Mahdavinezhad
Research Center for Molecular Medicine, Department of Genetics and Molecular Medicine, Hamadan University of Medical Sciences, Hamadan, Iran.
alimahdavin@gmail.com
Y
10.22088/acadpub.BUMS.6.2.3
Bladder cancer is the second most common cancer in the genitourinary tract, showing often recurrence and progresse into invasive states. Epigenetic changes, such as microRNA alteration are involved in bladder cancer tumorigenesis through a variety of signaling pathways. The epigenetic state depends on geographic and lifestyle conditions. The aim of this study was to investigate the expression level of microRNA-99a and microRNA-205 in bladder cancer in Iranian populations and to determine the relationship between their expressions with clinicophatological features. 36 patients with bladder cancer were included in the study. The control group was the healthy adjacent tissue of the same patients. Total RNA was extracted from approximately 50 mg tissue using TRIzol reagent. cDNA was synthesized and Real-Time PCR was carried out using specific primers. The Unisp6 rRNA was used as a reference gene. A significant decrease was found in the expression level of miR-99a in tumor samples, compared to healthy adjacent tissues (P<0.001). The increased expression level of miR-99a was significantly associated with muscle invasion (P=0.02). The receiver operating characteristic (ROC) analysis for miR-99a showed AUC value equal to 0.944, with specificity of 97%, sensitivity of 91%, and cut off value of 8.31 (P<0.001). A significant association was found between smoking and miR-99a (P=0.04) and miR-205 (P= 0.01) expression levels. Dramatic down-regulation of miR-99a in bladder cancer tissues confirmed the tumor suppressor role of miR-99a in bladder cancer. A higher amount of miR-99a expression was associated with invasive bladder cancer. According to ROC analysis, miR-99a could be considered as a valuable diagnostic biomarker.
microRNAs, neoplasms, Urinary bladder
http://ijmcmed.org/article-1-652-en.html
http://ijmcmed.org/article-1-652-en.pdf
Babol University of Medical Sciences
International Journal of Molecular and Cellular Medicine (IJMCM)
2251-9637
2251-9645
6
2
2017
6
1
Development of a Novel Anti-Adhesive Vaccine Against Pseudomonas aeruginosa Targeting the C-terminal Disulfide Loop of the Pilin Protein
96
108
EN
Sobhan
Faezi
Departments of Mycobacteriology and Pulmonary Research, Pasteur Institute of Iran, Tehran, Iran.
Sobhan.faezi@gmail.com
N
Ahmad Reza
Bahrmand
Departments of Mycobacteriology and Pulmonary Research, Pasteur Institute of Iran, Tehran, Iran.
Padideh79@yahoo.com
Y
Mehdi
Mahdavi
Departments of Immunology, Pasteur Institute of Iran, Tehran, Iran.
mahdavivac@gmail.com
N
Seyed Davar
Siadat
Departments of Mycobacteriology and Pulmonary Research, Pasteur Institute of Iran, Tehran, Iran.
siadat@pasteur.ac.ir
N
Iraj
Nikokar
Laboratory of Microbiology and Immunology of Infectious Diseases, Para Medicine Faculty, Guilan University of Medical Sciences, Rasht, Iran.
nikokariraj@yahoo.com
N
Soroush
Sardari
Biotechnology Research Center, Drug Design and Bioinformatics Group, Pasteur Institute of Iran, Tehran, Iran.
ssardari@hotmail.com
N
10.22088/acadpub.BUMS.6.2.4
The type IV pili (T4P) is a major virulence factor of Pseudomonas aeruginosa (P. aeruginosa) that is associated with primary adhesion, biofilm formation and twitching motility. This study focuses on the introduction of a novel biologically active subunit vaccine derived from the disulfide loop (DSL) of P. aeruginosa pilin. We investigated the expression of the novel PilA in-frame with pET26a vector, which contains three domains, that each domain contains three tandem repeats. The flexible (GGGGS) and (GGGGS)3 linkers were linked between the three tandem repeats and each pilA domain, respectively. The recombinant construct (pET26b/pilA) was transformed and expressed in Escherichia coli BL21 (DE3). The reactivity of specific antiserum against PilA was assessed by ELISA method. The biological activities of this candidate vaccine were evaluated by western blotting, opsonophagocytosis and twitching inhibition assays. The pET26b/pilA plasmid was confirmed by enzymatic digestion. The purified PilA protein was confirmed by immunoblot analysis. The checkerboard titration showed that the optimal dilution of the antibody to react with antigen was 1:8. The results of opsonophagocytosis assay revealed that the antibodies raised against PilA promoted phagocytosis of the PAO1 and 6266E strains, to some extent (17.5% and 16.3%, respectively), so that the twitching inhibition test confirmed this result. Taken together, these are preliminary results based on a first chimerical structure failure in order to induce antibodies that promote the opsonization and eradication of the pathogen. Therefore, the biological activity of the PilA protein showed that it should be introduced with other proteins or target antigens against P. aeruginosa in the future studies.
Pseudomonas aeruginosa, type IV pili, disulfide loop, vaccine, pET26b
http://ijmcmed.org/article-1-640-en.html
http://ijmcmed.org/article-1-640-en.pdf
Babol University of Medical Sciences
International Journal of Molecular and Cellular Medicine (IJMCM)
2251-9637
2251-9645
6
2
2017
6
1
Isolation and Characterisation of Actinobacteria from Algerian Sahara Soils with Antimicrobial Activities
109
120
EN
Harir
Mohamed
Biology of Microorganisms and Biotechnology Laboratory, University of Oran 1 Ahmed Ben Bella, Oran, Algeria.
Mohamedharir57@yahoo.fr
N
Bellahcene
Miloud
Faculty of Sciences, Natural and Life Sciences Department, Mohamed Boudiaf University, M’sila, Algeria.
bellahcene_miloud@yahoo.fr
N
Fortas
Zohra
Biology of Microorganisms and Biotechnology Laboratory, University of Oran 1 Ahmed Ben Bella, Oran, Algeria.
torfez2000@yahoo.fr
N
José María
José María García-Arenzana
Microbiología, Hospital Universitario Donostia, Donostia-San Sebastián, Spain.
arenzana@chdo.osakidetza.net
N
Antonio
Veloso
POLYMAT, University of the Basque Country UPV/EHU, Donostia-San Sebastian, Spain.
avelosofernandez@gmail.com
N
Susana
Rodriguez-Couto
Ceit-IK4, Water & Health Division, Donostia-San Sebastian, Spain.
srodriguez@ceiit.es
Y
10.22088/acadpub.BUMS.6.2.5
Extreme ecosystems can be a source of untapped micro-organisms able to produce novel bioactive compounds of industrial interest. Consequently, in this work, 32 Actinomycetes were isolated from 6 soil samples collected from Algerian Sahara in searching for untapped producers of novel antimicrobial compounds. All the isolates were further subjected to antimicrobial screening against pathogenic bacteria, yeast and fungi. The obtained results indicated that three of the isolates (named C, MS1 and 10) showed antimicrobial activities against most of the tested pathogenic micro-organisms. Therefore, these three promising isolates, previously identified as Streptomyces by morphological, biochemical and physiological methods, were selected for their subsequent identification by the whole cell matrix assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS) analysis. Thus, the isolates C, MS1 and 10 were identified as Streptomyces violaceoruber B263 UFL, Streptomyces albus B262 UFL, and Streptomyces badius B192 UFL, respectively. These results pointed out Actinomycetes from Sahara soils as potential sources of novel antimicrobial compounds. Also, MALDI-TOF MS showed to be a robust technique for bacteria identification.
Actinobacteria, antimicrobial activities, MALDI-TOF MS, Sahara soils, strain identification
http://ijmcmed.org/article-1-676-en.html
http://ijmcmed.org/article-1-676-en.pdf
Babol University of Medical Sciences
International Journal of Molecular and Cellular Medicine (IJMCM)
2251-9637
2251-9645
6
2
2017
6
1
Association between ACE (rs4646994), FABP2 (rs1799883), MTHFR (rs1801133), FTO (rs9939609) Gene Polymorphism and Type 2 Diabetes with Dyslipidemia
121
130
EN
Syed Tasleem
Raza
Department of Biochemistry, Era’s Lucknow Medical College and Hospital, Lucknow, India.
tasleem24@gmail.com
Y
Shania
Abbas
Department of Biochemistry, Era’s Lucknow Medical College and Hospital, Lucknow, India.
shania.abbas@gmail.com
N
Zeba
Siddiqi
Department of Medicine Era’s Lucknow Medical College and Hospital, Lucknow, India.
zsiddiqi9@gmail.com
N
Farzana
Mahdi
Department of Biochemistry, Era’s Lucknow Medical College and Hospital, Lucknow, India.
farzana.mahdi@gmail.com
N
10.22088/acadpub.BUMS.6.2.6
Diabetic dyslipidemia is one of the leading causes of coronary artery disease (CAD) death. Genetic and environmental factors play an important role in the development of type 2 diabetes mellitus (T2DM) and dyslipidemia. The present study was aimed to investigate the association of ACE (rs4646994), FABP2 (rs1799883), MTHFR (rs1801133) and FTO (rs9939609) genetic polymorphism in T2DM with dyslipidemia. Totally, 559 subjects including 221 T2DM cases with dyslipidemia, 158 T2DM without dyslipidemia and 180 controls were enrolled. ACE genetic polymorphism was evaluated by polymerase chain reaction (PCR), while MTHFR, FABP2, FTO genetic polymorphisms were evaluated by PCR and restriction fragment length polymorphism (RFLP). Significant association of ACE and MTHFR genetic polymorphisms were found in both group of cases [T2DM with dyslipidemia (P<0.001, and P=0.008, respectively) and T2DM without dyslipidemia (P=0.003, and P=0.010, respectively)] while FABP2 and FTO genetic polymorphisms were significantly associated with T2DM without dyslipidemia (P=0.038, and P= 0.019, respectively). This study concludes that ACE, FABP2, FTO and MTHFR genes are associated with T2DM. Additionally, it also seems that ACE and MTHFR genes might be further associated with the development of dyslipidemia in T2DM cases.
Genetic polymorphisms, type 2 diabetes mellitus (T2DM), dyslipidemia
http://ijmcmed.org/article-1-658-en.html
http://ijmcmed.org/article-1-658-en.pdf
Babol University of Medical Sciences
International Journal of Molecular and Cellular Medicine (IJMCM)
2251-9637
2251-9645
6
2
2017
6
1
A Novel Missense Mutation in the ALDH13 Gene Causes Anophthalmia in Two Unrelated Iranian Consanguineous Families
131
134
EN
Mohammadreza
Dehghani
Medical Genetics Research Center, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
dehghani.dr@gmail.com
N
Masoud
Dehghan Tezerjani
Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
masoud.msdco@gmail.com
N
Zahra
Metanat
Provincial Clinical Genetic Counseling Center, Zahedan University of Medical Sciences Zahedan, Iran.
dr_zahrametanat@yahoo.com
N
Mohammad Yahya
Vahidi Mehrjardi
Medical Genetics Research Center, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
mmvahidi@gmail.com
Y
10.22088/acadpub.BUMS.6.2.7
Anophthalmia or microphthalmia (A/M) is a rare group of congenital/developmental ocular malformations, characterized by absent or small eye within the orbit affecting one or both eyes. It has complex etiology with chromosomal, monogenic with high heterogeneity, and environmental causes. We performed genome SNP-array analysis followed by autozygosity mapping and sequencing in the members of two families in which three individuals are suffering from severe bilateral anophthalmia. The genetic analysis revealed a novel missense c.709G>A mutation in exon 7 of ALDH1A3 (aldehyde dehydrogenase 1 family member A3), causing a substi tution of glycine (Gly) to arginine (Arg) at residue 237. This study consolidates the importance of ALDH1A3 gene screening in autosomal recessive anophthalmi a. This variation may also be suggestive of a founder effect in the southeastern area of Iran.
Anophthalmia, ALDH1A3, consanguinity, autosomal recessive, SNP array
http://ijmcmed.org/article-1-663-en.html
http://ijmcmed.org/article-1-663-en.pdf