Babol University of Medical Sciences
International Journal of Molecular and Cellular Medicine (IJMCM)
2251-9637
2251-9645
4
4
2015
10
1
Adipose- Derived Stem Cells as a Tool for Dental Implant Osseointegration: an Experimental Study in the Dog
197
208
EN
Eriberto
Bressan
Department of Neurosciences, University of Padua, Padua, Italy.
guazoz@unipd.it
N
Daniele
Botticelli
Faculty of Dentistry, University of Medical Science, La Habana, Cuba.
sbricoli@unipd.it
N
Stefano
Sivolella
Department of Neurosciences, University of Padua, Padua, Italy.
Riccisa@unipd.it
N
Franco
Bengazi
Faculty of Dentistry, University of Medical Science, La Habana, Cuba.
Sicolella@unipd
N
Riccardo
Guazzo
Department of Neurosciences, University of Padua, Padua, Italy.
Botticelli@unipd.it
N
Luca
Sbricoli
Department of Neurosciences, University of Padua, Padua, Italy.
ferroni@unipd.it
N
Sara
Ricci
Department of Neurosciences, University of Padua, Padua, Italy.
gardin@unipd.it
N
Letizia
Ferroni
Department of Biomedical Sciences, University of Padua, Via Bassi 58, 35100 Padua, Italy.
barbara.zavan@unipd.it
N
Chiara
Gardin
Department of Biomedical Sciences, University of Padua, Via Bassi 58, 35100 Padua, Italy.
bressan@unipd.it
N
Joaquin
Urbizo Velez
Faculty of Dentistry, University of Medical Science, La Habana, Cuba.
N
Barbara
Zavan
Department of Biomedical Sciences, University of Padua, Via Bassi 58, 35100 Padua, Italy.
Y
The biological interaction between the jaw bones and dental implant is fundamental for the long-term success of dental implant placement. Nevertheless, the insufficient bone volume remains a major clinical problem, especially in case of immediate dental implant. Using a canine model, the present study proves the regenerative potential of adipose- derived stem cells (ADSCs) to repair peri-implant bone defects occurring in immediate dental implant placement. In six labradors, all mandibular premolars and the first molars were extracted bilaterally and three months later dental implants were installed with a marginal gap. The marginal defects were filled with hydroxyapatite (HA)-based scaffolds previously seeded with ADSCs. After one month of healing, specimens were prepared for histological and histomorphometric evaluations. Histological analyses of ground sections show that ADSCs significantly increase bone regeneration. Several new vessels, osteoblasts and new bone matrix were detected. By contrast, no inflammatory cells have been revealed. ADSCs could be used to accelerate bone healing in peri- implant defects in case of immediate dental implant placement.
Adipose- derived stem cells, alveolar bone loss, peri- implant bone defect, immediate dental implant, bone regeneration, hydroxyapatite
http://ijmcmed.org/article-1-358-en.html
http://ijmcmed.org/article-1-358-en.pdf
Babol University of Medical Sciences
International Journal of Molecular and Cellular Medicine (IJMCM)
2251-9637
2251-9645
4
4
2015
10
1
Expression Pattern of Neuronal Markers in PB-MSCs Treated by Growth Factors Noggin, bFGF and EGF
209
217
EN
Zahra
Fazeli
Department of Medical Genetics, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
zahrafazeli6@gmail.com
N
Masoumeh
Rajabibazl
Department of Medical Genetics, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
masoumeh_rajabibazl@yahoo.com
N
Siamak
Salami
Department of Clinical Biochemistry, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
salami.si@gmail.com
N
Nader
Vazifeh Shiran
Department of Clinical Biochemistry, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
nadershirani@yahoo.com
N
Sayyed Mohammad Hossein
Ghaderian
Department of Hematology, School of Allied Medical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
s.ghaderian@yahoo.co.uk
N
Mir Davood
Omrani
Department of Medical Genetics, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
davood_omrani@yahoo.co.uk
Y
Mesenchymal stem cells (MSCs) have the ability to differentiate into neuronal like cells under appropriate culture condition. In this study, we investigated whether MSCs derived from human peripheral blood (PB-MSCs) can differentiate into neuronal like cells by synergic effect of the growth factors EGF, bFGF and Noggin. For this purpose, the expression of five neuronal markers (Nestin, ;beta III tubulin, NFM, MAP2 and NSE) were evaluated in treated PB-MSCs by SYBR Green Real time PCR. The expression analysis showed a higher expression of ;beta- tubulin and NFM in treated BP-MSCs compared with untreated PB-MSCs as a control group. The expression of Nestin was also diminished in PB-MSCs treated with Noggin. This study suggested that the treatment of PB- MSCs with Noggin alongside with bFGF and EGF might differentiate these cells into neuronal lineage cells. The obtained results could be further developed for useful applications in regenerative medicine.
Mesenchymal Stem Cells, Differentiation, Neuronal markers, Noggin
http://ijmcmed.org/article-1-384-en.html
http://ijmcmed.org/article-1-384-en.pdf
Babol University of Medical Sciences
International Journal of Molecular and Cellular Medicine (IJMCM)
2251-9637
2251-9645
4
4
2015
10
1
An HIV-1 Mini Vaccine Induced Long-lived Cellular and Humoral Immune Responses
218
226
EN
Mehdi
Mahdavi
Department of Immunology, Pasteur Institute of Iran, Tehran, Iran.
mahdavivac@gmail.com
Y
Massoumeh
Ebtekar
Department of Immunology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
ebtekarm@modares.ac.ir
N
Zuhair Mohammad
Hassan
Department of Immunology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
hassan_zm@yahoo.co.uk
N
Sobhan
Faezi
Department of Mycobacteriology and Pulmonary Research, Pasteur Institute of Iran, Tehran, Iran.
sobhan.bcg@gmail.com
N
Hamidreza
Khorram Khorshid
Genetics Research Centre, University of Social Welfare and Rehabilitation Sciences, Tehran, Iran.
hrkhkh@yahoo.com
N
Morteza
Taghizadeh
Department of Medical Virology, Razi Vaccine and Serum Research Institute, Karaj, Iran.
taghizadeh.morteza@gmail.com
N
Keyhan
Azadmanesh
Department of Virology, Pasteur Institute of Iran, Tehran, Iran.
Azadmanesh@pasteur.ac.ir
N
Memory formation is the most important aspect of a vaccine which can guarantee long-lasting immunity and protection. The main aim of the present study was to evaluate the memory immune responses after immunization with a mini vaccine. Mice were immunized with human immunodeficiency virus-1 P24-Nef fusion peptide and then cellular and humoral immune responses were evaluated. In order to determine long-lived memory, immune responses were monitored for 20 weeks after final immunization. The results showed that the candidate vaccine induced proliferation and cytotoxic T lymphocyte responses and shifted cytokine patterns to T helper-1 profile. Evaluation of humoral immune responses also showed an increase in total peptide specific-IgG titer and a shift to IgG2a humoral response. Monitoring of immune responses at weeks 4, 12 and 20 after last immunization showed that immunologic parameters have been sustained for 20 weeks. Our findings support the notion that long-lived memory responses were achieved using a mini vaccine immunization.
BALB/c mice, HIV-1, long-lived immune responses, P24-Nef fusion peptide
http://ijmcmed.org/article-1-390-en.html
http://ijmcmed.org/article-1-390-en.pdf
Babol University of Medical Sciences
International Journal of Molecular and Cellular Medicine (IJMCM)
2251-9637
2251-9645
4
4
2015
10
1
Association between Helicobacter pylori cagA, babA2 Virulence Factors and Gastric Mucosal Interleukin-33 mRNA Expression and Clinical Outcomes in Dyspeptic Patients
227
234
EN
Heshmat
Shahi
Cellular and Molecular Research Center, Shahrekord University of Medical Sciences, Shahrekord, Iran.
heshmat.shahi@gmail.com
N
Somayeh
Reiisi
Department of Genetic, Faculty of basic science, Shahrekoed University, Shahrekord, Iran
s.reiisi@yahoo.com
N
Rasol
Bahreini
Department of Internal Medicine, Shahrekord University of Medical Sciences, Shahrekord, Iran
abr_abr021@yahoo.com
N
Nader
Bagheri
Cellular and Molecular Research Center, Shahrekord University of Medical Sciences, Shahrekord, Iran.
naderbagheri@gmail.com
N
Loghman
Salimzadeh
Cellular and Molecular Research Center, Shahrekord University of Medical Sciences, Shahrekord, Iran.
abrabr021@yahoo.com
N
Hedayatollah
Shirzad
Cellular and Molecular Research Center, Shahrekord University of Medical Sciences, Shahrekord, Iran.
shirzadeh@yahoo.com
Y
Helicobacter pylori (H. pylori) infection has been reported in more than half of the world human population. It is associated with gastric inflammation and noticeable infiltration of the immune cells to the stomach mucosa by several cytokines secretion. IL-1;beta, IL-18 have been shown to contribute to H. pylori induced gastritis, but the details of inflammation and association of virulence factors remain unclear. IL-1 cytokine family has a new additional cytokine, Interleukin-33 (IL-33), which is contemplated to have an important role for host defense against microorganisms. H. pylori virulence factors important in gastritis risk are the cag pathogenicity island (cag-PAI) and babA. This study evaluated IL-33 mucosal mRNA expression levels in infected and uninfected patients and its relationship with bacterial virulence factors cagA, babA2 and type of gastritis. Total RNA was extracted from gastric biopsies of 79 H. pylori-infected patients and 51 H. pylori-negative patients. Mucosal IL-33 mRNA expression levels in gastric biopsies were assessed using real-time PCR. Existence of virulence factors were detected by PCR. IL-33 mRNA expression was significantly higher in biopsies of H. pylori-infected patients compared to H. pylori-uninfected patients (P<0.0001). Also there was a direct relationship between virulence factor bab-A2 and enhancement in IL-33 mRNA expression. Furthermore, IL-33 mRNA expression level was significantly lower in chronic gastritis patients compared with patients with active gastritis (P<0.001). IL-33 may play a crucial role in the inflammatory response and induction of the chronic gastritis and severity of inflammatory changes in the gastric mucosa.
Helicobacter pylori, gastritis, interleukin-33, virulence factor
http://ijmcmed.org/article-1-423-en.html
http://ijmcmed.org/article-1-423-en.pdf
Babol University of Medical Sciences
International Journal of Molecular and Cellular Medicine (IJMCM)
2251-9637
2251-9645
4
4
2015
10
1
Molecular Characterization of Cryptosporidium Species in Children with Diarrhea in North West of Iran
235
239
EN
Behroz
Mahdavi poor
Department of Laboratory Science, Faculty of Paramedicine, Tabriz University of Medical Sciences, Tabriz, Iran.
Behroz.mahdavi@gmail.com
Y
Mohammad
Asgharzadeh
Biotechnology Research Center and Faculty of Paramedicine, Tabriz University of Medical Sciences, Tabriz, Iran.
Asgharzadehmo@yahoo.com
N
Esmaeel
Fallah
Department of Parasitology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran.
Fallahe@tbzmed.ac.ir
N
Kareem
Hatam-Nahavandi
Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
Karim.hatam@gmail.com
N
Jalil
Rashedi
Faculty of Paramedicine, Tabriz University of Medical Sciences, Tabriz, Iran.
Rashedijalil@gmail.com
N
Abdolhossein
Dalimi
Department of Medical Parasitology, School of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
dalimi_a@modares.ac.ir
N
Cryptosporidium is one of the most common causes of childhood diarrhea in developing countries. The aim of this randomized pilot study was to detect and characterize infective species and determine the genotypes of Cryptosporidium parasites in pediatric patients suffering from diarrhea in North West of Iran. A total of 113 fecal samples were collected from diarrheic children hospitalized in Tabriz Pediatric Hospital. The amplification of small subunit ribosomal RNA gene was performed using a nested polymerase chain reaction protocol and its products were digested using two restriction enzymes for Cryptosporidium species and genotype differentiation. Cryptosporidium oocysts were found in 2 (1.76%) children with diarrhea and restriction pattern revealed the presence of C.parvum bovine genotype in both positive fecal samples. The findings indicate that Cryptosporidium parvum is responsible for cryptosporidiosis in children in the study region and probably zoonotic transmission is the predominant route of parasite transmission.
Child, Cryptosporidium, genotype, PCR, Iran
http://ijmcmed.org/article-1-382-en.html
http://ijmcmed.org/article-1-382-en.pdf
Babol University of Medical Sciences
International Journal of Molecular and Cellular Medicine (IJMCM)
2251-9637
2251-9645
4
4
2015
10
1
Clonal Diversity in Multi Drug Resistant (MDR) Enterococci Isolated from Fecal Normal Flora
240
244
EN
Meysam
Hasannejad Bibalan
Department of Microbiology, Faculty of Medicine, Iran University of Medical Sciences, Tehran, IR Iran.
meysam_hasannejad@yahoo.com
N
Morteza
Eshaghi
Department of Microbiology, Faculty of Medicine, Iran University of Medical Sciences, Tehran, IR Iran.
meshaghi@yahoo.com
N
Javad
Sadeghi
Department of Microbiology, School of Medicine, Tehran University of Medical Sciences, Tehran, IR Iran.
j_sadeghi@yahoo.com
N
Mahla
Asadian
Department of Microbiology, Faculty of Medicine, Iran University of Medical Sciences, Tehran, IR Iran.
m_asadian@yahoo.com
N
Tahmineh
Narimani
Department of Microbiology, Faculty of Medicine, Iran University of Medical Sciences, Tehran, IR Iran.
t_narimani@yahoo.com
N
Malihe
Talebi
Department of Microbiology, Faculty of Medicine, Iran University of Medical Sciences, Tehran, IR Iran.
talebi.m@iums.ac.ir
Y
Enterococci are Gram positive and catalase- negative cocci that are found in the gastrointestinal tract of mammals and birds, and are readily isolated from soil, surface and waters. The aim of this study was to discriminate between Enterococcus isolates based on repetitive element sequence based –PCR (Rep-PCR) with the BOXA2R primer and their antibiotics profile. Enterococci isolates were obtained from 180 fecal samples. The isolates were identified by biochemical reaction and specific identification was confirmed by PCR with species specific primers. All isolates were subjected to Rep typing and antimicrobial susceptibility tests. Rep-PCR analysis of 180 isolates revealed 93 REP types with forty-five single types (ST1 to ST45) and forty-eight common types (CT1 to 48). Antibiotic susceptibility tests exhibited that 53 (29.4%), 43 (23.8%), 11 (6.1%) and 9 (5%) were resistant to erythromycin, tetracycline, gentamicin and ciprofloxacin respectively but among the isolates, sixteen were multi drug resistant (MDR). These MDR isolates showed 11 Rep types with seven single types and four common types. In addition, 81.2% of MDR isolates were from male subjects and the average age of these persons was more than fifty years. This study showed that 56.2% of MDR isolates were homogeneous with 95 % similarity, and high rate of resistance to tetracycline and erythromycin (81.2%) were observed in these isolates. The concern about these normal flora isolates are the pathogenic potential of these bacteria through the horizontal transfer of antibiotic resistance and virulence genes.
Enterococcus, Rep-PCR, antibiotic profile, normal flora
http://ijmcmed.org/article-1-370-en.html
http://ijmcmed.org/article-1-370-en.pdf
Babol University of Medical Sciences
International Journal of Molecular and Cellular Medicine (IJMCM)
2251-9637
2251-9645
4
4
2015
10
1
Autosomal Recessive Nonsyndromic Hearing Loss: A Case Report with a Mutation in TRIOBP Gene
245
247
EN
Majid
Fardaei
Department of Medical Genetics, Shiraz University of Medical Sciences, Shiraz, Iran.
mfardaei@sums.ac.ir
N
Shaghayegh
Sarrafzadeh
Department of Medical Genetics, Shahid Beheshti University of Medical sciences, Tehran, Iran.
soudehgh@yahoo.co.uk
N
Soudeh
Ghafouri-Fard
Department of Medical Genetics, Shahid Beheshti University of Medical sciences, Tehran, Iran.
s.ghafourifard@sbmu.ac.ir
Y
Mohammad
Miryounesi
Genomic Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
miryounesi@sbmu.ac.ir
N
Hearing loss (HL) is the most common sensory defect. Various genetic as well as environmental factors have been shown to contribute in it. More than 100 loci have been recognized to cause autosomal recessive nonsyndromic hearing loss (ARNSHL). Here, we report a 6-year old female patient with bilateral pre-lingual HL in whom a mutation has been identified in TRIOBP gene (c.6362C>T, S2121L). In silico analysis has shown that this variant is possibly pathogenic. Although several mutations have been detected in this gene in various populations, this is the first report identifying TRIOBP mutation in Iranian population. Consequently, the results of the present study may be of importance in genetic counseling.
Hearing loss, TRIOBP, mutation
http://ijmcmed.org/article-1-398-en.html
http://ijmcmed.org/article-1-398-en.pdf