OTHERS_CITABLE
Cellular response to ionizing radiation: A microRNA story
MicroRNAs (miRNAs) represent a class of small non-coding RNA molecules that regulate gene expression at the post-transcriptional level. They play a crucial role in diverse cellular pathways. Ionizing radiation (IR) is one of the most important treatment protocols for patients that suffer from cancer and affects directly or indirectly cellular integration. Recently it has been discovered that microRNA-mediated gene regulation interferes with radio-related pathways in ionizing radiation. Here, we review the recent discoveries about miRNAs in cellular response to IR. Thoroughly understanding the mechanism of miRNAs in radiation response, it will be possible to design new strategies for improving radiotherapy efficiency and ultimately cancer treatment.
http://ijmcmed.org/article-1-67-en.pdf
2013-07-03
178
184
Cellular response
ionizing radiation
microRNA
Mohammad
Halimi
1
Department of Biology, Faculty of Sciences, University of Guilan, Rasht, Iran.
AUTHOR
Mohsen
Asghari
2
Department of Biology, Faculty of Sciences, University of Guilan, Rasht, Iran.
AUTHOR
Reyhaneh
Sariri
3
Department of Biology, Faculty of Sciences, University of Guilan, Rasht, Iran.
AUTHOR
Dariush
Moslemi
4
Department of Radiation oncology, Babol University of Medical Sciences, Babol, Iran.
AUTHOR
Hadi
Parsian
5
Cellular and Molecular Biology Research Center, Babol University of Medical Sciences, Babol, Iran.
AUTHOR
ORIGINAL_ARTICLE
Rab11 is required for maintenance of cell shape via βPS integrin mediated cell adhesion in Drosophila
In eukaryotes, vesicle trafficking is regulated by the small monomeric GTPases of the Rab protein family. Rab11, (a subfamily of the Ypt/Rab gene family) an evolutionarily conserved, ubiquitously expressed subfamily of small monomeric Rab GTPases, has been implicated in regulating vesicular trafficking through the recycling of endosomal compartment. In an earlier communication, we have shown that Rab11 is required for cell adhesion, maintenance of cell shape and actin-cytoskeleton organization during Drosophila wing development. Here, we report that Rab11 is required for the maintenance of cell shape via βPS integrin mediated cell adhesion. Cuticle preparations of the embryos, when Rab11 is over-expressed or activity of Rab11 is reduced via a double-stranded RNAi line, show dorsal open phenotypes. Immuno-fluorescence and immuno-histochemical analyses on embryos in the same genetic backgrounds also affect the localization of βPS integrins from the adhesion site of leading edge and amnioserosa cells during the dorsal closure stages of embryogenesis as well as the cellular morphology (cell shape) of the lateral epidermal cells.
http://ijmcmed.org/article-1-60-en.pdf
2013-03-18
185
190
Amnioserosa
cellular morphology
cuticle
dorsal closure
Drosophila
Rab11
Tanmay
Bhuin
1
Cytogenetics Laboratory, Department of Zoology, Banaras Hindu University, Varanasi 221 005, India.
AUTHOR
Jagat
Kumar Roy
jkroy@bhu.ac.in
2
Cytogenetics Laboratory, Department of Zoology, Banaras Hindu University, Varanasi 221 005, India.
AUTHOR
ORIGINAL_ARTICLE
Central effects of camphor on GnRH and sexual hormones in male rat
In Persian traditional medicine is believed that camphor (a crystalline ketone obtained from cinnamomum camphora) is a suppressor of sexual behaviors. This study examined the central effects of camphor on sexual hormones (LH, FSH and testosterone) and GnRH plasma levels in male rat.
Male Wistar rats weighing 250-260gr were selected and divided into control (no treatment), sham (ICV injection of EtOH 10%) and treatment (ICV injection of camphor in three doses 4, 20, 40 µg/ 10µl in alcohol) groups. The serum samples were used for assaying of GnRH, LH, FSH and testosterone.
There were no significant differences in the levels of hormones between the groups of study.
Despite the central administration of camphor in hypothalamus - pituitary - gonad (HPG) axis, no significant differences were seen in sex hormone`s levels compared to the control. With this finding, it can be concluded that camphor may not effectively handle the axis via central pathway. These data recommend further studies of camphor on the HPG axis.
http://ijmcmed.org/article-1-71-en.pdf
2013-05-11
191
196
Camphor
GnRH
LH
FSH
Testosterone
Hypothalamus - Pituitary - Gonad (H-P-G) Axis
sima
shahabi
sima.shahabi@ymail.com
1
Cellular and Molecular Biology Research Center (CMBRC), Babol University of Medical Sciences, Babol, Iran.
AUTHOR
Seyed Gholam Ali
Jorsaraei
alijorsara@yahoo.com
2
Fatemeh Zahra Fertility and Infertility Reproductive Health Research Center, Babol University of Medical Sciences, Babol, Iran.
AUTHOR
Ali Akbar
Moghadamnia
moghadamnia@yahoo.com
3
Department of Physiology and Pharmacology, Babol University of Medical Sciences, Babol, Iran.
AUTHOR
Ebrahim
Zabihi
E_zabihi@yahoo.com
4
Cellular and Molecular Biology Research Center (CMBRC), Babol University of Medical Sciences, Babol, Iran.
AUTHOR
Seyed Mohsen
Aghajanpour Mir
mohsenaghajanpour@gmail.com
5
Cellular and Molecular Biology Research Center (CMBRC), Babol University of Medical Sciences, Babol, Iran.
AUTHOR
Seyedeh Narges
Mousavi Kani
6
Cellular and Molecular Biology Research Center (CMBRC), Babol University of Medical Sciences, Babol, Iran.
AUTHOR
Roghiyeh
Pourbagher
Pourbagher_17@yahoo.com
7
Cellular and Molecular Biology Research Center (CMBRC), Babol University of Medical Sciences, Babol, Iran.
AUTHOR
Seyed Ahmad
Hosseini
8
Student Research Committee, Babol University of Medical Sciences, Babol, Iran.
AUTHOR
Mohsen
Esmaili
drmesmaili
9
Student Research Committee, Babol University of Medical Sciences, Babol, Iran.
AUTHOR
Ali Asghar
Yoonesi
yoonesi325@yahoo.com
10
Student Research Committee, Babol University of Medical Sciences, Babol, Iran.
AUTHOR
Amin
Zarghami
11
Student Research Committee, Babol University of Medical Sciences, Babol, Iran.
AUTHOR
Farid
Alinezhad
12
Student Research Committee, Babol University of Medical Sciences, Babol, Iran.
AUTHOR
ORIGINAL_ARTICLE
A comparison between cytotoxicity induced by two resin based sealers (2Seal and AH Plus) in Saos-2 and MG-63 cell lines
The aim of this study was to evaluate and compare the cytotoxicity induced by two resin-based sealers, 2Seal and AH Plus, in two osteoblast-like cell lines, MG-63 and Saos-2. Using sterile discs of both sealers in complete media, 24- and 72-h extracts were prepared. The extracts were exchanged with Saos-2 or MG-63 cell culture media at 75% confluence, and after 24 h incubation, cell viability tests were performed for each extract and cell line using MTT and trypan blue dye exclusion assays. Corresponding incubated media were used as negative control groups. For both extracts and sealers, cytotoxicity was observed in both cell lines. For Saos-2, there was no statistical difference in toxicity between the sealers for either extract (p > 0.05). For MG-63, the 2Seal 24-h extract and the AH Plus 72-h extract had greater cytotoxicity than the other extracts (p < 0.05(. Both AH Plus and 2Seal demonstrated significant cytotoxicity in these two cell lines. In contrast to 2Seal, the cytotoxicity of AH Plus in the MG-63 cell line increased with extraction time from 24 to 72 h. The AH Plus and 2Seal 24-h extracts showed different levels of cytotoxicity in the MG-63 cell line, while in the Saos-2 cell line there were no detectable differences. This may reflect higher sensitivity of the MG-63 cell line compared to Saos-2 toward cytotoxicity induced by these two sealers, or different kinetics of toxicant release from the sealers.
http://ijmcmed.org/article-1-63-en.pdf
2013-04-09
197
202
Cytotoxicity
AH Plus
2Seal
Osteosarcoma
Saos-2
MG-63
Maryam
Ehsani
m.ehsani@mubabol.ac.ir
1
Dental Materials Research Center, School of Dentistry, Babol University of Medical Sciences, Babol, Iran.
AUTHOR
Ebrahim
Zabihi
e.zabihi@mubabolac.ir
2
Cellular and Molecular Biology Research Center, Babol University of Medical Sciences, Babol, Iran.
AUTHOR
Hamed
Gharouee
3
Department of Endodontics, School of Dentistry, Babol University of Medical Sciences, Babol, Iran.
AUTHOR
ORIGINAL_ARTICLE
Evaluation of PKM2 and MAPK8IP2 polymorphism in Ameloblastic Carcinoma: A retrospective quantitative study
Ameloblastic carcinoma (AC) is a rare malignant epithelial odontogenic tumor that histologically retains the features of ameloblastic differentiation and exhibits cytological features of malignancy in the primary or recurrent tumor. It may develop within a preexisting ameloblastoma or arise de novo or from an odontogenic cyst. Epidemiological evidence shows that human cancer is generally caused by genotoxic factors, genes involved in the susceptibility of cancer, including those involved in metabolism or detoxification of genotoxic environment and those controlling DNA replication. Nowadays, gene polymorphism has an important role in development of malignant tumor. We report a case series study of ameloblastic carcinoma and ameloblastoma to show the role of PKM2 and MAPK8IP2 polymorphisms in these tumors. The DNA was extracted separately from specimens in paraffin sections of the tumor. Polymorphism of these genes was determined by PCR-RFLP (Polymerase Chain Reaction-Restriction fragment length polymorphism) method. The allele distributions of all samples were in Hardy-Weinberg equilibrium. The genotype and allele distribution in these genes were not statistically different between patients and controls.
http://ijmcmed.org/article-1-65-en.pdf
2013-03-17
203
208
Ameloblastic carcinoma
PKM2
MAPK8IP2
polymorphism
Abbas
Khodayari
1
Department of Oral and Maxillofacial Surgery, Dental Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
AUTHOR
Sayyed Mohammad Hossein
Ghaderian
sghaderian@yahoo.co.uk
2
Department of Medical Genetics, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
AUTHOR
Mohammad
Jafarian
3
Department of Oral and Maxillofacial Surgery, Dental Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
AUTHOR
Alireza
Jahangirnia
alireza_omfs@yahoo.com
4
Department of Oral and Maxillofacial Surgery,Shahid Beheshti University of Medical Sciences, Tehran, Iran.
AUTHOR
Alireza
Nayebi
5
Department of Oral and Maxillofacial Surgery, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
AUTHOR
Fahimeh
Akhlaghi
6
Department of Oral and Maxillofacial Surgery, Dental Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
AUTHOR
Nasim
Taghavi
7
Department of oral and maxillofacial pathology, dental faculty,Shahid beheshty university of medical science,Tehran, Iran.
AUTHOR
Reza
Akbarzadeh Najar
rz_akbarzade@yahoo.com
8
Department of Genetics, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
AUTHOR
Sanaz
Tabarestani
sanaztf@gmail.com
9
Department of Genetics, Shahid Beheshti university of Medical Sciences, Tehran,Iran.
AUTHOR
Arash
Khojasteh
10
Department of Oral and Maxillofacial Surgery, Dental Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
AUTHOR
Sarah
Aghabozorg Afjeh
s.afjeh@yahoo.com
11
Department of Genetics, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
AUTHOR
ORIGINAL_ARTICLE
Molecular detection of Integron genes and pattern of antibiotic resistance in Pseudomonas aeruginosa strains isolated from intensive care unit, Shahid Beheshti Hospital, North of Iran
Pseudomonas aeruginosa is one of the most important pathogens that causes nosocomial infections and shows high level of antibiotic resistance. Integrons are one of the transposable elements in bacteria and their role in antibiotic resistance has been well demonstrated. The aim of this study was a molecular characterization of the integron genes and the determination of the resistance or sensitivity pattern to ceftizoxime, ceptazidime. cephotaxim, amikacin, ofloxacin, imipenem, cefepime, ticarcillin, gentamicin, ciprofloxacin, cefazolin and ceftriaxone antibiotics in P. aeruginosa strains isolated from Intensive Care Units (ICU), Shahid Beheshti Hospital, North of Iran. This cross-sectional study was performed from 2011 to 2012. Totally, fifty four P. aeruginosa strains were isolated from ICU at Shahid-Beheshti hospital, Babol, North of Iran. The bacteria were diagnosed based on mobility, pigment production, growth in 420 C, oxidase and catalase tests. PCR analysis was carried out to detect integron genes using hep 35 and hep 36 primers. Also, disk diffusion method was performed to evaluate antibiotic susceptibility of the bacteria using ceftizoxime, ceftazidime, cephotaxime, amikacin, ofloxacin, imipenem, cefepime, ticarcillin, gentamicin, ciprofloxacin, cefazolin and ceftriaxone antibacterial reagents. This study revealed that 20 (37%) P. aeruginosa isolates had integron genes. The antibiotic susceptibility test showed that 52 (96.3%) of the isolates were multidrug-resistant. 12 out of 54 isolated bacteria were resistant to all antibiotics tested. All bacteria were resistant to cefepime (100%) and the highest resistance rate was seen to ceftazidime 92.6% fallowed by cefazolin 96.3%. The lowest resistance rate was observed to ciprofloxacin 38.9%, ofloxacin 44.4%, amikacin 46.3% and ticarcillin 48.1%. According to this study, P. aeruginosa isolates showed high level of antibiotic resistance and the presence of integrons in these strains can explain the influence of these genes in resistance creation. There was a significant association between resistance to cefotaxime, amikacin, ofloxacin, imipenem, ticarcillin, gentamicin and the presence of integrons.
http://ijmcmed.org/article-1-56-en.pdf
2013-05-27
209
216
Pseudomonas aeruginosa
integrons
drug resistance
Fatemeh
Moradian
m.moradiyan20@yahoo.com
1
Department of Microbiology Science and Research Branch Islamic Azad University, Guilan, Iran.
AUTHOR
Elaheh
Ferdosi Shahandashti
elaheh.ferdosi@yahoo.com
2
Department of Microbiology and Immunology, Babol University of Medical Sciences, Babol Iran.
AUTHOR
Zahra
Moulana
zmoulana@yahoo.com
3
Infectious Diseases and Tropical Medicine Research Center, Babol University of Medical Sciences, Babol, Iran.
AUTHOR
Massomeh
Moradian kouchaksaraei
moradiyan20@yahoo.com
4
Department of Microbiology Science and Research Branch Islamic Azad University, Fars, Iran.
AUTHOR
Fariba
Asgharpour
5
Faculty of Para-Medicine; Babol University of Medical Sciences, Babol, Iran.
AUTHOR
Ali
Mojtahedi
alimojtahedi@yahoo.com
6
Department of Microbiology, Faculty of Medicine, Guilan University Campus, Rasht, Iran.
AUTHOR
Ramazan
Rajabnia
Ramazan69@yahoo.com
7
Infectious Diseases and Tropical Medicine Research Center, Babol University of Medical Sciences, Babol, Iran.
AUTHOR
ORIGINAL_ARTICLE
Relationship of Podoplanin and Glutathione S-transferases T1 Expression with Laryngeal Cancer
The aim of this study is to determine whether there is a role of podoplanin and glutathione S-transferases T1 (GST-T1) expression in laryngeal squamous cell carcinoma.
The study was completed with 33 patients and gene expression analysis was performed by qRT–PCR. The podoplanin and GST-T1 expression patterns were analyzed to determine their correlation with clinicopathologic parameters of laryngeal cancer.
Of all patients, 20 had supraglottic, and the remaining 13 had glottic laryngeal cancer. Increased expression of podoplanin was found in 14 tumor tissues, but GST-T1 expression was not detected.
Podoplanin expression did not show any prediction for regional metastasis, thyroid cartilage invasion, lymphatic vessel invasion or tumor differentiation for laryngeal cancer, also there were no significant differences in podoplanin expression between glottic and supraglottic regions, but extracapsullar extension is almost statistically significant (p=0,05).
http://ijmcmed.org/article-1-53-en.pdf
2013-03-17
217
224
Podoplanin
GST-T1
laryngeal carcinoma
biomarker
squamous cell carcinoma
Deniz
Kanliada
ecoskun@istanbul.edu.tr
1
Department of ORL and Head&Neck Surgery, Istanbul Faculty of Medicine, Istanbul, Turkey.
AUTHOR
Ender
Coskunpinar
ecoskunpinar@gmail.com
2
Department of Molecular Medicine, Institute of Experimental Medicine, Istanbul University, Istanbul, Turkey.
AUTHOR
Kadir Serkan
Orhan
ksorhan@yahoo.com
3
Department of ORL and Head&Neck Surgery, Istanbul Faculty of Medicine, Istanbul, Turkey.
AUTHOR
Yasemin
Musteri Oltulu
yaseminmusteri@yahoo.com
4
Department of Molecular Medicine, Institute of Experimental Medicine, Istanbul University, Istanbul, Turkey.
AUTHOR
Mehmet
Celik
drdeniz.ent@gmail.com
5
Department of ORL and Head&Neck Surgery, Istanbul Faculty of Medicine, Istanbul, Turkey.
AUTHOR
Ayse
Eren
ayseren_84@hotmail.com
6
Department of Molecular Medicine, Institute of Experimental Medicine, Istanbul University, Istanbul, Turkey.
AUTHOR
Ilhan
Yaylim
ilhanyaylim@gmail.com
7
Department of Molecular Medicine, Institute of Experimental Medicine, Istanbul University, Istanbul, Turkey.
AUTHOR
Kemal
Değer
kdeger@e-kolay.net
8
Department of ORL and Head&Neck Surgery, Istanbul Faculty of Medicine, Istanbul, Turkey.
AUTHOR
CASE_STUDY
Balanced Chromosomal Rearrangement in Recurrent Spontaneous Abortions: A Case Report
One of the major causes of spontaneous abortion before the fourth month of pregnancy is chromosomal abnormalities. We report an unusual case of a familial balanced chromosomal translocation in a consanguineous couple who experienced 4 spontaneous abortions. Chromosomal studies were performed on the basis of G-banding technique at high resolution and revealed 46, XX, t (16 6) (p12 q26) and 46, XY, t (16 6) (p12 q26) in both partners, which induced such pregnancy complications.
Chromosomal balanced translocation is one of the most common causes of recurrent spontaneous abortions (RSA). In such cases prenatal diagnosis (PND) during the 16th week of gestation is strongly recommended.
http://ijmcmed.org/article-1-54-en.pdf
2013-03-17
225
228
Chromosomal abnormality
Spontaneous abortion
Chromosomal translocation
Recurrent miscarriage
Case report
Ahmadreza
Zarifian
zarifianar891@mums.ac.ir
1
Student Research Assembly, Mashhad University of Medical Sciences, Iran.
AUTHOR
Zeinab
Farhoodi
farhoodiz891@mums.ac.ir
2
Student Research Assembly, Mashhad University of Medical Sciences, Iran.
AUTHOR
Roya
Amel
amelr891@mums.ac.ir
3
Student Research Assembly, Mashhad University of Medical Sciences, Iran.
AUTHOR
Salmahe
Mirzaee
mirzaees1@mums.ac.ir
4
Department of Medical Genetics, Faculty of Medicine, Mashhad University of Medical Sciences, Iran.
AUTHOR
Mohammad
Hassanzadeh-Nazarabad
nazarabadim@mums.ac.ir
5
Department of Medical Genetics, Faculty of Medicine, Mashhad University of Medical Sciences, Iran.
AUTHOR