en
jalali
1398
8
1
gregorian
2019
11
1
8
4
online
1
fulltext
en
Diagnostic Value of Plasma Long Non-coding RNA HOTTIP as a Non-invasive Biomarker for Colorectal Cancer ( A Case- Control Study)
Long non-coding RNAs (lncRNAs) associated with various cancers, including colorectal cancer (CRC), could be collected from body fluids easily. Our aims were to determine the expression level of HOTTIP lncRNA in plasma samples of healthy individuals and CRC patients as well as their relationship with clinico-pathological characteristics of patients. First, total RNA was extracted from the plasma samples of 100 subjects including 50 patients and 50 age and sex matched healthy persons. Then, gene expression was measured using real-time PCR technique. The sensitivity and specificity of HOTTIP dysregulation in CRC and healthy individual’s plasma was measured by receiver operating characteristic (ROC) analysis. As compared with healthy controls, HOTTIP lncRNA was over expressed in a statistically significant manner in plasma samples of patients (P = 0.001). Significant relationship between HOTTIP expression and positive family history of CRC was observed, too (P = 0.04). The ROC curve analysis showed an AUC value of 0.775, a specificity of 82%, a sensitivity of 76%, with a cut off value equal to 2.40 (P = 0.001). HOTTIP transcript can be proposed as a new biomarker for early diagnosis due to the increased expression in plasma samples of patients with CRC and the relatively high sensitivity and specificity.
Biomarker, colorectal neoplasms, long non-coding RNAs, lncRNA HOTTIP
240
246
http://ijmcmed.org/browse.php?a_code=A-10-314-5&slc_lang=en&sid=1
2019/08/7
1398/5/16
2020/01/12
1398/10/22
Soheila
Ali akbar- Esfahani
Department of Molecular Medicine & Genetics, Hamadan University of Medical Sciences, Hamadan, Iran.
soheilaaa.esfahany@gmail.com
00319475328460016627
00319475328460016627
No
Morteza
Karimipoor
Molecular Medicine Department, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran.
mortezakarimi@yahoo.com
00319475328460016628
00319475328460016628
No
Fatemeh
Bahreini
Department of Molecular Medicine & Genetics, Hamadan University of Medical Sciences, Hamadan, Iran.
f.bahreini@yahoo.com
00319475328460016629
00319475328460016629
No
Ali Reza
Soltanian
Modelling of Non communicable Diseases Research Center, School of Public Health, Hamadan University of Medical Sciences, Hamadan, Iran.
arsoltanian@yahoo.com
00319475328460016630
00319475328460016630
No
Najme
Aletaha
Department of Gastroenterology, Imam Khomeini Hospital, Tehran University of Medical Science, Tehran, Iran.
dr.aletaha@gmail.com
00319475328460016631
00319475328460016631
No
Ali
Mahdavinezhad
Research center for Molecular Medicine, Department of Molecular Medicine and Genetics, Hamadan University of Medical Sciences, Hamadan, Iran.
alimahdavin@gmail.com
00319475328460016632
00319475328460016632
Yes
en
Association of MicroRNA-155 rs767649 polymorphism with Susceptibility to Preeclampsia
Preeclampsia (PE) is a multifactorial disorder. Several studies showed that micro RNAs may play a critical role in PE pathogenesis. We aimed to investigate for the first time the association of mir-155rs767649 polymorphism with PE. Eighty patients with preeclampsia and 80 normal subjects were enrolled in the study. Serum expression levels of mature mir-155were evaluated using real-time PCR, and mir-155 rs767649 (T/A) polymorphism was genotyped using TaqMan SNP genotyping. There was a significant difference between the expression level of mir-155 in cases (5.86 ± 3.11) in comparison with controls (0.58 ± 0.30) (P<0.0001). Also,the minor allele of rs767649 was significantly associated with increased risk of PE [Recessive model: adjusted Odds ratio (OR) = 5.240, 95% confidence interval (CI) = (1.999-13.733),P= 0.001]. There was a significant difference between different genotypes according to expression levels of mir-155 in PE (P<0.0001) with high expression levels in TA genotype (7.10 ± 3.11 ). Mir-155 may play a critical role in PE pathogenesis. The obtained data suggest that a minor allele of rs767649 might be a predisposing factor for PE.
MicroRNA, mir-155, single nucleotidepolymorphism, preeclampsia
247
257
http://ijmcmed.org/browse.php?a_code=A-10-2041-1&slc_lang=en&sid=1
2019/08/72019/09/30
1398/7/8
2020/01/122020/04/9
1399/1/21
Shymaa
E. Ayoub
Department of Biochemistry and Molecular Biology, Fayoum University, Al Fayoum, Egypt.
ssa05@fayoum.edu.eg
00319475328460016633
00319475328460016633
Yes
Olfat
G. Shaker
Department of Medical Biochemistry and Molecular Biology, Faculty of Medicine, Cairo University, Giza, Egypt.
Olfatshaker@yahoo.com
00319475328460016634
00319475328460016634
No
Mostafa
Y. Abdelwahed
Department of Physiology, Faculty of Medicine, Zagazig University, ElZagazig, Egypt.
mya01@fayoum.edu.eg
00319475328460016635
00319475328460016635
No
Naglaa
A. Ahmed
Department of Physiology, Faculty of Medicine, Zagazig University, ElZagazig, Egypt.
naahmed@nu.edu.sa
00319475328460016636
00319475328460016636
No
Hazem
G. Abdelhameed
Department of Gynecology and Obstetrics, Faculty of Medicine, Fayoum University, Al Fayoum, Egypt.
Hazem.galal@rocketmail.com
00319475328460016637
00319475328460016637
No
Almandouh
H. Bosilah
Department of Gynecology and Obstetrics, Faculty of Medicine, Fayoum University, Al Fayoum, Egypt.
Msobhy7@yahoo.com
00319475328460016638
00319475328460016638
No
Sheren
R. Mohammed
Department of Biochemistry and Molecular Biology, Fayoum University, Al Fayoum, Egypt.
sherenKaddafy @yahoo.com
00319475328460016639
00319475328460016639
No
en
Utilization of Whole Exome Sequencing in Lethal Form of Multiple Pterygium Syndrome: Identification of Mutations in Embryonal Subunit of Acetylcholine Receptor
The acetylcholine receptor (AChR) is a member of the superfamily of transmitter-gated ion channels having a critical role in controlling electrical signals between nerves and muscle cells. Disruptive mutations in genes encoding different subunits of AChR result in multiple pterygium syndrome (MPS), which can be associated with a severe prenatally lethal presentation. This study aimed to investigate the etiology of lethal MPS (LMPS) in two consanguineous families with a history of miscarriages. DNA was extracted from a tissue sample of two aborted fetuses (probands) from two different families with a history of spontaneous miscarriages. Parental peripheral blood samples were collected for confirmatory analysis and follow-up testing. Whole-exome sequencing (WES) was performed on DNA from the probands. The results were confirmed and segregated by Sanger sequencing. Moreover, protein structure evaluations were accomplished. We identified a homozygous frameshift mutation of c.753_754delCT (p.V253fs*44) and a homozygous missense mutation of c.715C>T (p.Arg239Cys) in the CHRNG gene. Both aborted fetuses had pterygium, severe arthrogryposis, and fetal hydrops with cystic hygroma, being compatible with LMPS. The heterozygous state was confirmed in parents for both CHRNG variants. Likewise, CHRNG mutation was predicted to display the damaging effects by lowering the number of helixes and modifying the surface electrostatic potential. The present study identified rare sequence variants in the CHRNG gene in aborted fetuses from consanguineous couples with recurrent miscarriage history. WES is a comprehensive and cost-effective approach to study heterogeneous diseases including MPS. Such findings can improve our knowledge of MPS databases, particularly for genetic counseling of high-risk families and preimplantation genetic diagnosis.
Multiple pterygium syndromes, whole-exome sequencing, CHRNG,recurrent abortion
258
270
http://ijmcmed.org/browse.php?a_code=A-10-2138-1&slc_lang=en&sid=1
2019/08/72019/09/302019/11/5
1398/8/14
2020/01/122020/04/92020/02/22
1398/12/3
Tahere
Nazari
Department of Medical Genetics, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
Tahereh_nazary@yahoo.com
00319475328460016640
00319475328460016640
No
Ali
Rashidi-Nezhad
Maternal, Fetal and Neonatal Research Center, Tehran University of Medical Sciences, Tehran, Iran.
ali_rashidinejad@yahoo.com
00319475328460016641
00319475328460016641
No
Maziar
Ganji
Department of Medical Genetics, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran,Iran.
maziar.ganji92@gmail.com
00319475328460016642
00319475328460016642
No
Zahra
Rezaei
Department of Medical Genetics, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
Rezaei.genetic@gmail.com
00319475328460016643
00319475328460016643
No
Saeed
Talebi
Department of Medical Genetics, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.
talebisaid@yahoo.com
00319475328460016644
00319475328460016644
No
Nasrin
Ghasemi
Abortion Research Centre, Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
n479g@yahoo.co.uk
00319475328460016645
00319475328460016645
No
Javad
Tavakkoly Bazzaz
Department of Medical Genetics, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
tavakkolybazzazj@tums.ac.ir
00319475328460016646
00319475328460016646
Yes
en
A Cardiac Troponin T Biosensor Based on Aptamer Self-assembling on Gold
In this study, a sensitive and accurate aptasensor was designed for early detection of myocardial infarction through the determination of troponin T (TnT). The successful immobilization of a specific aptamer sequence on the surface of gold that had a high affinity toward TnT was accomplished. TnT was electrochemically quantified. The results indicated that the aptasensor detected TnT in a range of 0.05-5 ng mL and with a detection limit of 0.01 ng/mL. The performance of the aptasensor was investigated by analyzing 99 human serum samples. Both diagnostic specificity and sensitivity of the aptasensor were found to be 95%. The use of the designed aptamer-based biosensor could be an essential achievement in health policy, preventing deaths caused by myocardial infarction, and reducing patients with heart failure. The extensive use of this aptamer-based biosensor can also reduce costs, enhance speed, and improve accuracy in the diagnosis of TnT as an important myocardial infarction biomarker.
Myocardial infarction, aptasensing, biomarker, bioelectrochemical detection
271
282
http://ijmcmed.org/browse.php?a_code=A-10-2256-1&slc_lang=en&sid=1
2019/08/72019/09/302019/11/52019/12/28
1398/10/7
2020/01/122020/04/92020/02/222020/04/13
1399/1/25
Masoud
Negahdary
Nanomedicine and Nanobiology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran; Yazd Cardiovascular Research Center, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
masoudnegahdary@yahoo.com
00319475328460016647
00319475328460016647
No
Mostafa
Behjati-Ardakani
Yazd Cardiovascular Research Center, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
dr_behjati@yahoo.com
00319475328460016648
00319475328460016648
No
Hossein
Heli
Nanomedicine and Nanobiology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
hheli7@yahoo.com
00319475328460016649
00319475328460016649
No
Naghmeh
Sattarahmady
Nanomedicine and Nanobiology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran; Department of Medical Physics, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
nsattar@sums.ac.ir
00319475328460016650
00319475328460016650
Yes
en
Proliferation, Characterization and Differentiation Potency of Adipose Tissue-Derived Mesenchymal Stem Cells (AT-MSCs) Cultured in Fresh Frozen and non-Fresh Frozen Plasma
Mesenchymal stem cells (MSCs) have unique properties, including high proliferation rates, self-renewal, and multilineage differentiation ability. Their characteristics are affected by increasing age and microenvironment. This research aimed to determine the proliferation, characteristics and differentiation capacity of adipose tissue-derived (AT)-MSCs at many passages with different media. The cell proliferation capacity was assayed using trypan blue. MSCs characterization (CD90, CD44, CD105, CD73, CD11b, CD19, CD34, CD45, and HLA-DR) was performed by flow cytometry, and cell differentiation was determined by specific stainings. Population doubling time (PDT) of AT-MSCs treated with fresh frozen plasma (FFP) and non-FFP increased in the late passage (P) (P15 FFP was 22.67 ± 7.01 days and non-FFP was 19.65 ± 2.27 days). Cumulative cell number was significantly different between FFP and non-FFP at P5, 10, 15. AT-MSCs at P4-15 were positive for CD90, CD44, CD105, and CD73, and negative for CD11b, CD19, CD34, CD45, and HLA-DR surface markers. AT-MSCs at P5, 10, 15 had potential toward adipogenic, chondrogenic, and osteogenic differentiation. Therefore, PDT was affected by increased age but no difference was observed in morphology, surface markers and differentiation capacity among passages. Cumulative cell number in FFP was higher in comparison with non-FFP in P5, 10, 15. Our data suggest that FFP may replace FBS for culturing MSCs.
AT-MSCs, multilineage differentiation, PDT, proliferation, surface marker
283
293
http://ijmcmed.org/browse.php?a_code=A-10-2224-1&slc_lang=en&sid=1
2019/08/72019/09/302019/11/52019/12/282019/12/13
1398/9/22
2020/01/122020/04/92020/02/222020/04/132020/04/2
1399/1/14
Wahyu
Widowati
Medical Research Center, Faculty of Medicine, Maranatha Christian University, Bandung, West Java, Indonesia
wahyu_w60@yahoo.com
00319475328460016651
00319475328460016651
Yes
Rachmawati
Noverina
1. Animal and Stem Cells Laboratory, PT Bio Farma (Persero), Bandung 40161, West Java, Indonesia; 2. Doctoral Program, Faculty of Medicine, Universitas Padjadjaran, Bandung, West Java, Indonesia
ayoex.wireni@yahoo.com
00319475328460016652
00319475328460016652
No
Wireni
Ayuningtyas
1. Animal and Stem Cells Laboratory, PT Bio Farma (Persero), Bandung 40161, West Java, Indonesia; 2. Magister Program, Faculty of Medicine, Universitas Padjadjaran, Bandung, West Java, Indonesia
ayoex.wireni@yahoo.com
00319475328460016653
00319475328460016653
No
Dedy
Kurniawan
Animal and Stem Cells Laboratory, PT Bio Farma (Persero), Bandung 40161, West Java, Indonesia
rudededy@gmail.com
00319475328460016654
00319475328460016654
No
Hanna
Sari Widya Kusuma
Biomolecular and Biomedical Research Center, Aretha Medika Utama, Bandung, West Java, Indonesia
hannasariw@amubbrc.co.id
00319475328460016655
00319475328460016655
No
Seila
Arumwardana
Biomolecular and Biomedical Research Center, Aretha Medika Utama, Bandung, West Java, Indonesia
seila.wardana91@gmail.com
00319475328460016656
00319475328460016656
No
Dwi
Surya Artie
Biomolecular and Biomedical Research Center, Aretha Medika Utama, Bandung, West Java, Indonesia
artie.dwisurya@gmail.com
00319475328460016657
00319475328460016657
No
Ika
Adhani Sholihah
Biomolecular and Biomedical Research Center, Aretha Medika Utama, Bandung, West Java, Indonesia
ikaadhani18@gmail.com
00319475328460016658
00319475328460016658
No
Rr. Anisa
Siwianti Handayani
Biomolecular and Biomedical Research Center, Aretha Medika Utama, Bandung, West Java, Indonesia
anisa.siwi@gmail.com
00319475328460016659
00319475328460016659
No
Dian
Ratih Laksmitawati
Faculty of Pharmacy, Pancasila University, Jakarta, Indonesia
dianratih.ffup@gmail.com
00319475328460016660
00319475328460016660
No
Ratih
Rinendyaputri
National Institute of Health Research and Development, Ministry of Health, Jakarta, Indonesia
ratihr79@yahoo.com
00319475328460016661
00319475328460016661
No
Rilianawati
Rilianawati
Agency for the Assesment and Application of Technology, Ministry of Research and Technology, Serpong, Indonesia
liliaabbas@gmail.com
00319475328460016662
00319475328460016662
No
Ahmad
Faried
Department of Neurosurgery and Stem Cell Working Group, Faculty of Medicine, Universitas Padjadjaran-Dr. Hasan Sadikin Hospital, Bandung, West Java, Indonesia
faried.fkup@gmail.com
00319475328460016663
00319475328460016663
No
en
AMELX Gene Association with Dental Caries in Iranian Adults
Dental decay is a disease that is greatly affected by environmental components, but recently there have been an increasing number of documents supporting a genetic factor in the development of caries. The purpose of this study was to examine the association between dental caries and single-nucleotide polymorphisms in the AMELX gene. This research was carried out on 360 individuals of both sexes, who were referred to the dental school at the Shiraz University of Medical Sciences. In this research, individuals aged from 20–65 years were divided into two groups: controls (decayed, missed, or filled teeth (DMFT) ≤ 5; n = 180) and cases (DMFT ≥ 14; n = 180). The tetra-primer ARMS-PCR technique was performed for genotyping the DNA extracted from blood cells. Analysis of the AMELX rs946252 polymorphism showed that the T allele of rs946252 was a significant protective factor against dental caries in Iranian adults (T vs. C: OR = 0.70, 95% CI: 0.49–0.98, P = 0.04). We demonstrated the significant differences in the genotype frequencies under two genetic models: overdominant (TC vs. TT + CC: OR 0.35, 95% CI 0.19–0.64, P = 0.0006) and recessive (CC vs. TC + TT: OR 2.57, 95% CI 1.39–4.76, P = 0.002). Our results show that the SNPs of the AMELX gene may be related with susceptibility to dental caries in Iranian adults.
Dental caries, DMFT, AMELX, rs946252, Tetra-primer ARMS-PCR
294
299
http://ijmcmed.org/browse.php?a_code=A-10-1900-1&slc_lang=en&sid=1
2019/08/72019/09/302019/11/52019/12/282019/12/132019/06/29
1398/4/8
2020/01/122020/04/92020/02/222020/04/132020/04/22020/01/22
1398/11/2
Fatemeh
Koohpeima
Department of Operative Dentistry, Biomaterial Research Center, School of Dentistry, Shiraz University of Medical Sciences, Shiraz, Iran.
koohpeima.f@gmail.com
00319475328460016664
00319475328460016664
No
Maryam
Derakhshan
Department of Operative Dentistry, Biomaterial Research Center, School of Dentistry, Shiraz University of Medical Sciences, Shiraz, Iran.
allahyari.mahmood@yahoo.com
00319475328460016665
00319475328460016665
No
Mohammad Javad
Mokhtari
Young Researchers and Elite Club, Department of Biology, Zarghan Branch, Islamic Azad University, Zarghan, Iran.
mj.mokhtari@gmail.com
00319475328460016666
00319475328460016666
Yes
en
Post-Mortem Diagnosis of Heme Oxygenase-1 Deficiency by Whole Exome Sequencing in an Iranian Child
Heme oxygenase-1 (HO-1) is an inducible enzyme involved in the catalysis of heme conversion into biliverdin. We describe a patient with a novel stop-gain mutation in the HMOX1 coding sequence resulting in HO-1 deficiency.
Hemeoxygenase-1 deficiency, post-mortem diagnosis, HO-1 gene, Iranian child
300
306
http://ijmcmed.org/browse.php?a_code=A-10-2025-1&slc_lang=en&sid=1
2019/08/72019/09/302019/11/52019/12/282019/12/132019/06/292019/09/21
1398/6/30
2020/01/122020/04/92020/02/222020/04/132020/04/22020/01/222020/04/10
1399/1/22
Fatemeh
Tahghighi
Children's Medical Center, Pediatrics Center of Excellence, Tehran, Iran; Department of Pediatrics, Tehran University of Medical Sciences,Tehran, Iran.
f-tahghighi@sina.tums.ac.ir
00319475328460016667
00319475328460016667
No
Nima
Parvaneh
Division of Allergy and Clinical immunology, Department of Pediatrics, Tehran University of Medical Sciences,Tehran, Iran; Research Center for Immunodeficiencies, Tehran University of Medical Sciences, Tehran, Iran.
nparvaneh@sina.tums.ac.ir
00319475328460016668
00319475328460016668
No
Vahid
Ziaee
Department of Pediatrics, Tehran University of Medical Sciences,Tehran, Iran; Pediatric Rheumatology Research Group, Rheumatology Research Center, Tehran University of Medical Sciences, Tehran, Iran.
Ziaee@tums.ac.ir
00319475328460016669
00319475328460016669
Yes